农药对秀丽隐杆线虫毒性效应及其机制的研究进展

随着大量农药被广泛的使用并最终汇聚到环境介质中,对生态环境和人体健康产生潜在影响。秀丽隐杆线虫(Caenorhabditis elegans)是土壤中最丰富的后生动物,在土壤生态系统中具有重要地位,并作为一种重要的模式生物广泛应用于环境毒理学研究。本文从秀丽隐杆线虫常用的毒理学研究方法入手,以致死率、生长发育、运动行为、繁殖、活性氧自由基(ROS)水平、细胞凋亡水平、相关基因表达量和蛋白水平等作为测试指标,归纳总结农药对秀丽隐杆线虫的衰老性、神经及生殖系统的毒性效应,分析探索其毒性机理,并展望了未来的研究重点。

Review on Toxicology of Pesticides in the Nematode Caenorhabditis elegans

A large number of pesticides were widely used and eventually accumulated in environmental matrices, which caused the adverse impacts in the ecosystems and humans health. Caenorhabditis elegans (C. elegans), a free-living nematode, was found abundantly in the terrestrial ecosystem. The C. elegans was an excellent model animal in environmental toxicology research, and had been successfully used in the environmental and toxicological studies. In the present review, we discussed that pesticide caused ageing, neurotoxicity, reproductive toxicity with endpoints of development, locomotion behavior, reproduction, reactive oxygen species (ROS) levels, cell apoptosis, gene expression, and protein level in C. elegans, and further introduced the toxicological mechanisms of pesticides to C. elegans. Finally, we proposed three research perspectives with aspects of toxicology assessment, multigenerational toxicity, and molecular mechanisms.