蛋白组学分析揭示出阿特拉津对非洲爪蟾的生殖毒性源于其诱导生殖细胞凋亡并干扰紧密连接蛋白和代谢相关信号通路

阿特拉津可对雄性脊椎动物生殖系统造成不良影响，但其中分子机制尚不明了。本文作者将非洲爪蟾(Xenopus laevis)暴露于100 ppb的阿特拉津120 d，利用同位素标记相对和绝对定量(iTRAQ)技术检测了非洲爪蟾睾丸和卵巢中蛋白图谱的变化，结果显示100 ppb阿特拉津可影响爪蟾发育，延迟和阻碍雄性细精管的形成。组学分析显示，睾丸中143种以及卵巢中121种蛋白均表达异常，这些蛋白与细胞凋亡、细胞间紧密连接以及代谢途径相关。
精选自Xiuping Chen, Jiamei Wang, Haojun Zhu, Jiatong Ding and Yufa Peng. Proteomics analysis of Xenopus laevis gonad tissue following chronic exposure to atrazine. Environmental Toxicology and Chemistry: Volume 34, Issue 8, pages 1744–1750, August 2015. DOI: 10.1002/etc.2980
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Proteomics analysis of Xenopus laevis gonad tissue following chronic exposure to atrazine

Atrazine is the most commonly detected pesticide contaminant in ground and surface water. Previous studies have shown that atrazine is an endocrine disruptor owing to its adverse effects on the male reproductive system in several vertebrates, but very few molecular mechanisms for these effects have been revealed. In the present study, Xenopus laevis were exposed to 100 ppb of atrazine for 120 d, and then the isobaric tags for relative and absolute quantitation (iTRAQ) technique was used to detect global changes in protein profiles of the testes and ovaries. The results showed that 100 ppb of atrazine exposure adversely affected the growth of X. laevis and did not induce hermaphroditism but delayed or prevented the development of male seminiferous tubules. Proteomic analysis showed that atrazine altered expression of 143 and 121 proteins in the testes and ovaries, respectively, and most of them are involved in cellular and metabolic processes and biological regulation based on their biological processes. In addition, apoptosis, tight junctions, and metabolic pathways were significantly altered in the atrazine-treated gonads. Based on the above results, it is postulated that the reproductive toxicity of atrazine may be the result of disruption of tight junctions and metabolic signaling pathways and/or induction of apoptosis in germ cells.
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