铬暴露导致的秀丽线虫多重毒性的世代间比较

利用模式生物秀丽线虫(Caenorhabditis elegans)对铬(Cr)暴露导致的多重毒性及其在世代间的可传递性进行了研究.发育、生殖、运动行为、行为可塑性与寿命等方面的参数被用于多重毒性的分析.结果表明,Cr能够导致线虫出现多种表型和行为缺陷.低浓度(2.5μmol·L-1)Cr暴露即可导致发育、生殖与寿命上的严重缺陷,而高浓度(75和200μmol·L-1)Cr暴露还可以导致运动行为与行为可塑性上的严重缺陷.而且,大部分高浓度Cr暴露导致的缺陷可以从暴露当代传递到后代,使后代仍然呈现出严重的表型与行为缺陷.在这些后代中,各表型与行为缺陷只能得到有限的恢复,或没有明显的恢复.因此,就秀丽线虫而言,Cr暴露导致的各表型与行为缺陷很大程度上可以从暴露当代传递到后代个体中.

Comparison of Multi-Biological Toxicities between Chromium Exposed Caenorhabditis elegans and Their Progeny

Multi-biological toxicities in chromium exposed animals and their progeny were analyzed using model organism Caenorhabditis elegans. Endpoints of development, reproduction, locomotion behavior, behavioral plasticity and lifespan were selected for the assay of multiple toxicities from chromium exposure. Our data suggested that the chromium exposure could result in multi-biological defects of phenotypes and behaviors. Low-concentration(2.5μmol·L-1) chromium exposure caused severe defects of development, reproduction and lifespan. High-concentration(75 and 200μmol·L-1)chromium exposure could further result in severe defects of locomotion behaviors and behavioral plasticity. Moreover, most of these toxicities caused by high-concentration chromium exposure could be transferred from exposed animals to their progeny. In progeny animals, the phenotypic and behavioral defects could be only partially or very slightly rescued. Therefore, the multi-biological defects caused by chromium exposure could be largely transferred from exposed animals to their progeny in C. elegans.