葡多酚对口服乙醇小鼠肝细胞PCNA和Bcl-2表达的影响

为观察葡多酚(GPC)对小鼠乙醇性肝损伤的防护作用,将每天经口灌胃给予4 g·(kg·bw)-1乙醇的小鼠同时分别给予不同剂量的GPC,30 d后处死小鼠;取肝组织用MTT法检测各组小鼠肝细胞的增殖活性,用免疫组化法和图像分析方法检测PCNA和Bcl-2表达水平.结果显示:GPC高剂量组16 h的肝细胞增殖活性为0.4...

Effect of Grape Procyanidins on Expression of PCNA and Bcl\|2 Protein of Hepatic Cells in Mice Damaged by Ethanol

To study the protective effect of grape procyanidins(GPC) on liver injury induced by ethanol in mice,the mice were orally given 4 g·(kg·bw)-1 ethanol with different doses of grape procyanidins once a day.After 30 days,the mice were killed.The proliferation activity of the hepatic cells was determined by MTT assay;the levels of PCNA and Bcl-2 protein expression in the cell were measured using immunohistochemistry method and image analysis method.Results show that the proliferation activity of the high-dose GPC group was 0.423±0.125,significantly higher than that of the ethanol group(p< 0.05).The proliferation index of PCNA protein and the expression rates of Bcl-2 protein of the high-dose GPC group were 39.26% and 33.78%,respectively,and those of the ethanol group were 9.5% and 9.22%,respectively.The OD value of PCNA and Bcl-2 protein of the high-dose GPC group were 0.449±0.041 and 0.376±0.048,respectively,those of the ethanol group were 0.342±0.040 and 0.281±0.036,respectively.There were significant differences between these groups(p< 0.05).It indicated that GPC had inhibitory effect on the decrease of proliferation activity and Bcl-2 protein expression in mice induced by ethanol.It had protective effect on liver injury induced by ethanol in mice.