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| 重金属暴露铜锈环棱螺全组织均一化cDNA文库构建及表达序列标签特征分析 | |
| 引用本文： | 安立会,郑丙辉,王静波,张雷,李子成,付青,王春燕,赵兴茹,陈浩,王丽靖,尚晶晶.重金属暴露铜锈环棱螺全组织均一化cDNA文库构建及表达序列标签特征分析[J].生态毒理学报,2011,6(3):281-288. |
| 作者姓名： | 安立会 郑丙辉 王静波 张雷 李子成 付青 王春燕 赵兴茹 陈浩 王丽靖 尚晶晶 |
| 作者单位： | 1. 中国环境科学研究院环境基准与风险评估国家重点实验室,北京100012;中国环境科学研究院国家环境保护河口与海岸带重点实验室,北京100012 2. 北京市水产技术推广站,北京,100021 3. 中国环境科学研究院国家环境保护河口与海岸带重点实验室,北京,100012 4. 中国环境科学研究院环境基准与风险评估国家重点实验室,北京,100012 |
| 基金项目： | 国家水体污染控制与治理科技重大专项(2009ZX07528-03); 中国新型环境问题(2009467109) |
| 摘 要： | 构建cDNA文库是寻找生物新功能基因的有效手段.采用SM ART技术构建了重金属铜和镉暴露铜锈环棱螺全组织均一化cDNA文库,文库库容为1.78×106克隆,重组率大于99%.从初始文库中随机挑取6000个克隆进行测序,获得5 473个高质量表达序列标签(ESTs)序列.经聚类分析得到3 961个Unigene(平均长... |
| 关 键 词： | 铜锈环棱螺 重金属 均一化cDNA文库 表达序列标签 |
| 收稿时间： | 2010/11/10 0:00:00 |
| 修稿时间： | 2011/4/28 0:00:00 |
Construction of Normalized cDNA Library and Analysis of Expressed Sequenced Tags Properties of Bellamya Aeruginosa |
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| An Lihui,Zheng Binghui,Wang Jingbo,Zhang Lei,Li Zicheng,Fu Qing,Wang Chunyan,Zhao Xingru,Chen Hao,Wang Lijing,Shang Jingjing.Construction of Normalized cDNA Library and Analysis of Expressed Sequenced Tags Properties of Bellamya Aeruginosa[J].Asian Journal of Ecotoxicology,2011,6(3):281-288. | |
| Authors: | An Lihui Zheng Binghui Wang Jingbo Zhang Lei Li Zicheng Fu Qing Wang Chunyan Zhao Xingru Chen Hao Wang Lijing Shang Jingjing |
| Institution: | 1,21.State key Laborary of Environmental Criteria and Risk Assment,Chinese Research Academy for Environment Sciences,Beijing,100012,China2.State Environmental Protection Key Laboratory of Estuarine and Coastal Research,Chinese Research Academy for Environment Sciences,Beijing,100012,China3.Beijing Aquiculture Technology Extension Station,Beijing,100021,China |
| Abstract: | Construction of normalized cDNA library is an available tool for identifying novel genes of organisms. In the present study, a cDNA library of Bellamya Aeruginosa exposed to heavy metals through SMART technique was constructed. The tier of cDNA library was 1.78×106 plaques, with a recombinant rate of more than 99%. 6 000 clones selected randomly and 5 473 expressed sequenced tags (ESTs) were obtained. After processing, a total of 3 961 unigenes comprising 897 contigs and 3 064 singlets were obtained, and the redundancy was 27.63%. Analysis of EST using COG, GO and KEGG showed that a large group of genes related to growing development, signal transduction mechanisms of cells, defense mechanisms were obtained in the present cDNA library. The normalized cDNA library developed in the present study provides a valuable resource for further research on functional genes and ecotoxicology to Bellamya Aeruginosa . |
| Keywords: | Bellamya Aeruginosa heavy metal normalized cDNA library expressed sequenced tags (ESTs) |
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