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纳米硫硒化镉对小鼠肾脏和脑组织SOD活力和MDA含量的影响
引用本文:项晓玲,李岩,田熙科,杨超,吴曲,常青,袭著革,杨旭.纳米硫硒化镉对小鼠肾脏和脑组织SOD活力和MDA含量的影响[J].生态毒理学报,2008,3(2):168-173.
作者姓名:项晓玲  李岩  田熙科  杨超  吴曲  常青  袭著革  杨旭
作者单位:1. 华中师范大学生命科学学院环境科学实验室,武汉,430079
2. 中国地质大学材料科学与化学工程学院,武汉,430079
3. 军事医学科学院卫生学环境医学研究所,天津,300050
基金项目:国家高技术研究发展计划(863计划)
摘    要:为了探讨纳米硫硒化镉(CdSeS)对小鼠肾脏和脑组织的急性氧化损伤作用,将20只雄性昆明小鼠随机分成4组,采用尾部静脉注射进行一次性染毒,3个染毒组分别注入0.1、0.2、0.4mg·mL-1的纳米CdSeS粉末(20~30nm)悬液1mL,对照组注入等体积生理盐水.染毒3d后对肾脏和脑组织匀浆中超氧化物歧化酶(SOD)活力和丙二醛(MDA)含量分别进行测定,从而检测纳米CdSeS对肾脏和脑组织的急性氧化损伤作用.结果显示,随着纳米CdSeS染毒浓度的升高,小鼠肾脏和脑组织中SOD活力呈逐渐降低趋势,而MDA含量呈逐渐升高趋势,均显示出一定的剂量-效应关系;较高浓度组(肾:ρCdSeS≥0.2mg·mL-1;脑:ρCdSeS≥0.4mg·mL-1)SOD活力、MDA含量与对照具有显著性差异(p<0.05,p<0.01),而较低浓度组则没有显著性差异(p>0.05).以上结果提示纳米CdSeS能够对小鼠肾脏和脑组织造成氧化损伤,并且能穿过血脑屏障作用于脑部。

关 键 词:纳米硫硒化镉  超氧化物歧化酶  丙二醛  脂质过氧化反应  氧化损伤  肾脏    小鼠
文章编号:1673-5897(2008)2-168-06
收稿时间:2007/11/24 0:00:00
修稿时间:2007年11月24

Effects of CdSeS Nanocrystal on SOD Activities and MDA Contents in Mouse Kidney and Brain Tissue
XIANG Xiao-ling,LI Yan,TIAN Xi-ke,YANG Chao,WU Qu,CHANG Qing,YANG Xu,XI Zhu-ge.Effects of CdSeS Nanocrystal on SOD Activities and MDA Contents in Mouse Kidney and Brain Tissue[J].Asian Journal of Ecotoxicology,2008,3(2):168-173.
Authors:XIANG Xiao-ling  LI Yan  TIAN Xi-ke  YANG Chao  WU Qu  CHANG Qing  YANG Xu  XI Zhu-ge
Abstract:The present study was conducted in order to study the effects of CdSeS nanocrystal(20~30nm)on the acute oxidative damage in mouse kidney and brain tissue.20 Kunming mice were divided randomly into 4 treatment groups(5 mice in each group)and were exposed to different concentrations of CdSeS(0,0.1,0.2,0.4mg·mL-1)by a single tail intravenous injection.After 3d exposure,the superoxide dismutase(SOD)activities and malondialdehyde(MDA)contents in kidney and brain tissue were measured as indicators of oxidative damage.Results showed that the SOD activities in mouse kidney and brain tissue were decreased gradually and MDA contents were increased gradually with the increase of CdSeS concentrations.Dose-effect relationships were found between CdSeS concentrations and SOD activities,MDA contents in the mouse kidney and brain tissue.There were significant differences(p<0.05,p<0.01)in the SOD activities and MDA contents between the high concentration groups(kidney:ρCdSeS≥0.2mg·mL-1;brain:ρCdSeS≥0.4mg·mL-1)and the control group,while no significant differences were found between the low concentration groups and the control group.These results suggested that CdSeS nanocrystal could induce the acute oxidative damage in mouse kidney and brain tissue,and it could cross the blood brain barrier.
Keywords:CdSeS nanocrystal  superoxide dismutase(SOD)  malondialdehyde(MDA)  lipid peroxidation  oxidative damage  kidney  brain  mouse
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