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PFOS青春期暴露对成年期雄性大鼠的生殖毒性
引用本文:李洪志,刘洁婷,张春雷,初彦辉,赵冰海.PFOS青春期暴露对成年期雄性大鼠的生殖毒性[J].生态毒理学报,2012,7(4):434-438.
作者姓名:李洪志  刘洁婷  张春雷  初彦辉  赵冰海
作者单位:牡丹江医学院黑龙江省抗纤维化生物治疗重点实验室,牡丹江,157011
基金项目:国家自然科学基金(81102149H2607);黑龙江省普通高等学校青年学术骨干支持计划(1252G065);牡丹江医学院科学技术研究项目(2011-15)
摘    要:为探究青春期的PFOS暴露对成年后的SD大鼠的生殖毒性,对出生后第21天(PND21)的SD大鼠经口灌胃不同剂量的PFOS(5、10和20mg·kg~(-1)),连续染毒7d,在出生后第56天(PND56)时,对各染毒组SD大鼠的体质量、精子数量、血清中的睾酮浓度,以及睾丸间质细胞睾酮合成的相关基因mRNA水平进行了检测。结果显示,10mg·kg~(-1)剂量组大鼠体质量较对照组明显下降(P<0.01);精子数量在10mg·kg~(-1)和20mg·kg~(-1)剂量组明显降低(P<0.05);血清中睾酮浓度随着PFOS剂量的加大有明显下降的趋势,20mg·kg~(-1)剂量组显著低于对照组(P<0.05);类/胆固醇相关基因star和cyp11α1的mRNA表达水平明显下调。研究表明,青春期的PFOS暴露会导致睾酮合成途径中相关因子的功能缺失,破坏成熟睾丸间质细胞的功能,致使睾酮水平降低,并抑制精子生成,从而破坏生殖系统的功能。

关 键 词:全氟辛烷磺酸(PFOS)  SD大鼠  青春期暴露  生殖毒性  睾丸间质细胞  睾酮
收稿时间:2012/2/23 0:00:00
修稿时间:2012/5/17 0:00:00

Reproductive Toxicity of PFOS Adolescent Exposure to Adult Male Rats
Li Hongzhi,Liu Jieting,Zhang Chunlei,Chu Yanhui and Zhao Binghai.Reproductive Toxicity of PFOS Adolescent Exposure to Adult Male Rats[J].Asian Journal of Ecotoxicology,2012,7(4):434-438.
Authors:Li Hongzhi  Liu Jieting  Zhang Chunlei  Chu Yanhui and Zhao Binghai
Institution:Key Laboratory of Anti\|Fibrosis Biotherapy of Heilongjiang Province, Mudanjiang Medical University, Mudanjiang 157011, China;Key Laboratory of Anti\|Fibrosis Biotherapy of Heilongjiang Province, Mudanjiang Medical University, Mudanjiang 157011, China;Key Laboratory of Anti\|Fibrosis Biotherapy of Heilongjiang Province, Mudanjiang Medical University, Mudanjiang 157011, China;Key Laboratory of Anti\|Fibrosis Biotherapy of Heilongjiang Province, Mudanjiang Medical University, Mudanjiang 157011, China;Key Laboratory of Anti\|Fibrosis Biotherapy of Heilongjiang Province, Mudanjiang Medical University, Mudanjiang 157011, China
Abstract:To explore the reproductive toxic effect of adolescent exposure to perfluorooctane sulfonate(PFOS) on adult male rats,the adolescent SD rats on postnatal day 21(PND21) were exposed to different doses of PFOS(5,10 and 20 mg·kg~(-1)) by gavage for 7 days.Several indexes of SD rats on PND56 in each exposure group were measured,including body mass,sperm count,serum testosterone concentration,and mature Leydig cell mRNA levels associated with testosterone synthesis.Results indicated that compared with the control,the body mass of rats significantly decreased in 10 mg·kg~(-1) exposure group(P< 0.01);sperm counts were significantly lower in 10 mg·kg~(-1) and 20 mg·kg~(-1) exposure groups(P< 0.05);serum testosterone concentration exhibited a clear decreasing pattern with increasing PFOS dose,and the concentration was significantly lower in 20 mg·kg~(-1) exposure group(P< 0.05);the mRNA levels of steroid/cholesterol-related genes,star and cyp11α 1,significantly decreased.It is demonstrated that PFOS adolescent exposure would cause the loss of function related to testosterone synthesis,thus resulting in destroying mature Leydig cell function,reducing the testosterone concentration in SD rats and inhibiting sperm production,and finally destroy the function of reproductive system.
Keywords:perfluorooctane sulfonate  Sprague Dawley rats  adolescent exposure  reproductive toxicology  Leydig cell  testosterone
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