水暴露下氟在西伯利亚鲟稚鱼硬骨和软骨中的积累和消除

采用静态水暴露试验方法,设置0.26(对照)、3.1、7.8、18.7、51.8mg·L-1氟离子(F-)5个试验组暴露西伯利亚鲟稚鱼,暴露90d后转移至清水中培养30d,检测了这2个阶段鱼体硬骨和软骨中氟离子含量,探讨了氟离子在西伯利亚鲟稚鱼硬骨和软骨中的积累与消除规律,以及氟离子对鱼体生长的影响.结果表明,随着氟离子浓度的增加和暴露时间的延长,氟在西伯利亚鲟稚鱼硬骨和软骨中的含量显著增加,暴露90d时各暴露组硬骨和软骨中氟含量均显著高于对照组;西伯利亚鲟稚鱼暴露在较高浓度氟离子(7.8、18.7和51.8mg·L-1)中90d,生长速度(体重)显著下降;经过30d清水培养后,较高浓度暴露组(18.7和51.8mg·L-1)西伯利亚鲟稚鱼硬骨的氟含量显著下降.由此结论:氟在西伯利亚鲟稚鱼硬骨和软骨组织中具有较高的生物蓄积作用;转移至清水中饲养后,蓄积的高浓度的氟可从硬骨中排出.

The Bioaccumulation and Elimination of Fluoride in Bone and Cartilage of Juvenile Siberian Sturgeon (Acipenser baerii) after Waterborne Fluoride Exposure under Environmental Related Concentration

A 120-day growth trial was conducted to determine the accumulation and elimination of fluoride ions (F-) in juvenile Siberian sturgeon (Acipenser baerii). Fish were first exposed to each measured F-concentration of 3.1, 7.8, 18.7 and 51.8mg·L-(1added as NaF), along with a control group(0.26mg·L-1)for 90 days, followed by a 30-day fresh water exposure period. Fish growth was determined and F-contents in bone and cartilage were analyzed in different tissues every 30 days. Results showed that fish growth was inhibited significantly for groups exposed to 7.8, 18.7 and 51.8mg·L -1 compared with the control group. F-concentrations in bone and cartilage showed dose -response and time-effect. After 90-day exposure, significant increases in F-content were observed in bone and cartilage of Siberian sturgeon exposed to concentrations above 3.1mg·L-1. F-content in bone significantly decreased in the 18.7 and 51.8mg·L-1 exposure groups after 30 days reared in fresh water. It is summarized that F-can be accumulated in bone and cartilage in Siberian sturgeon from water and F-can be eliminated from bone after reared in fresh water.