多壁碳纳米管存在环境下Pb、Zn对斑马鱼毒性的变化(The Change of the Toxicity of Pb and Zn on Zebrafish in the Case of the Existence of Multi-Walled Carbon Nanotubes)

研究了重金属铅、锌对斑马鱼的急性毒性效应及在人工碳纳米材料——多壁碳纳米管存在环境下,铅、锌对斑马鱼毒性的变化.结果显示,随着铅(Pb(NO3)2)、锌(ZnSO4)浓度的增加以及染毒时间的延长,斑马鱼死亡率逐渐增加;单一多壁碳纳米管悬液(10mg·L-1)对斑马鱼无明显毒性效应.Pb和Zn对斑马鱼24h、48h和96h的LC50分别为5.38mg·L-1、3.99mg·L-1、3.83mg·L-1和26.37mg·L-1、21.39mg·L-1、20.62mg·L-1;在多壁碳纳米管存在条件下,二者对斑马鱼24h、48h和96h的LC50分别为2.74mg·L-1、2.26mg·L-1、2.15mg·L-1和21.85mg·L-1、17.17mg·L-1、16.77mg·L-1.多壁碳纳米管存在条件下铅、锌对斑马鱼的LC50显著降低,提示碳纳米材料可能会增加重金属对水生生物的毒性.

The Change of the Toxicity of Pb and Zn on Zebrafish in the Case of the Existence of Multi-Walled Carbon Nanotubes

The paper mainly studied that the toxicity effect of heavy metals lead, zinc on zebrafish, and the impact of their lethal effect on zebrafish in the case of the existence of manufactured nanomaterials —multi-walled carbon nanotubes (MWCNTs). Results showed that the LC50 values in 24h, 48h and 96h of the lead solution(Pb(NO3)2)and zinc solution (ZnSO4)were 5.38mg·L-1,3.99mg·L-1,3.83mg·L-1 and 26.37mg·L-1,21.39mg·L-1,20.62mg·L-1,respectively; Under the conditions of existence of nano-materials (10mg·L-1), the LC50 values in the same time of the two toxic substances were 2.74mg·L-1, 2.26mg·L-1,2.15mg·L-1 and 21.85mg·L-1,17.17mg·L-1,16.77mg·L-1,respectively. With the increase of heavy metal concentrations and exposure time, the mortality of the zebrafish increased ;the suspension of MWCNTs had no obvious toxic effect on the zebrafish;but under the conditions of existence of nano-materials, the LC50 values of Pb, Zn on the zebrafish decreased significantly . Nano-materials could increase the toxicity of heavy metals to aquatic organisms.