| 溴阻燃剂对肝细胞的毒性 |
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| 引用本文： | 那广水,孙丽娟,张雯雯,王惠国.溴阻燃剂对肝细胞的毒性[J].生态毒理学报,2012,7(5):570-573. |
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| 作者姓名： | 那广水 孙丽娟 张雯雯 王惠国 |
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| 作者单位： | 1. 国家海洋环境监测中心国家海洋局近岸海域生态环境重点实验室,大连,116023
2. 大连大学生命科学与技术学院,大连,116622 |
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| 基金项目： | 海洋公益性科研专项(201105013) |
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| 摘 要： | 为评价四溴联苯醚(2,2,4,4-tetrabromodipheny lether,PBDE-47)、五溴联苯醚(2,2',4,4',5-pentabromodiphenylether,PBDE-99)、九溴联苯醚(polybrominated dipheny lethers206,PBDE-206)、六溴环十二烷(hexabromocyclododecane,HBCD)、四溴双酚A(tetrabromobisphenol A,TBBPA)对人肝细胞(LO2)的体外细胞毒性。将5种溴阻燃剂与人肝细胞LO2共同培养,采用四甲基偶氮噻唑蓝比色法(MTT法)检测5种溴阻燃剂对LO2细胞的相对增殖率,并按照GB/T16886.5-2003/ISO10993-5:1999标准评价5种溴阻燃剂对LO2的细胞毒性。体外实验表明,5种溴阻燃剂对LO2细胞均具有抑制作用,其中PBDE-47、PBDE-99和HBCD呈现剂量依赖关系。
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| 关 键 词： | 四溴联苯醚 五溴联苯醚 九溴联苯醚 六溴环十二烷 四溴双酚A LO_2细胞 |
| 收稿时间： | 2012/7/21 0:00:00 |
| 修稿时间： | 2012/9/29 0:00:00 |
Hepatotoxicity of Brominated Flame Retardants |
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| Na Guangshui,Sun Lijuan,Zhang Wenwen and Wang Huiguo.Hepatotoxicity of Brominated Flame Retardants[J].Asian Journal of Ecotoxicology,2012,7(5):570-573. |
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| Authors: | Na Guangshui Sun Lijuan Zhang Wenwen and Wang Huiguo |
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| Institution: | Marine Chemistry Department, National Marine Environmental Monitoring Center, Dalian 116622, China;College of Life Sciences and Biotechnology, Dalian University, Dalian 116622, China;College of Life Sciences and Biotechnology, Dalian University, Dalian 116622, China;College of Life Sciences and Biotechnology, Dalian University, Dalian 116622, China |
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| Abstract: | To test the toxic effects of 2, 2, 4, 4-tetrabromodiphenyl ether (PBDE-47), 2,2',4,4',5-pentabromodiphenyl ether (PBDE-99), polybrominated diphenyl ethers 206 (PBDE-206), hexabromocyclododecane (HBCD) and tetrabromobisphenol A (TBBPA) on normal human hepatocytes (LO2) in vitro, the human liver cell line LO2 was co-cultured with these five brominated flame retardants respectively. The inhibition rate of LO2 cells was detected by MTT (3-(4,5-Dimethylthia zol-2-yl)-2,5-diphenyltetrazolium bromide) assay, and the cytotoxicity was evaluated in accordance with the criterion of GB/T16886.5-2003/ISO10993-5: 1999. As a result, these five brominated flame retardants showed inhibiting effect on the LO2 cells in vitro experiments. PBDE-47, PBDE-99 and HBCD showed dose-response relation. |
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| Keywords: | 2 2 4 4-tetrabromodiphenyl ether (PBDE-47) 2 2' 4 4' 5-pentabromodiphenyl ether (PBDE-99) polybrominated diphenyl ethers 206 (PBDE-206) hexabromocyclododecane (HBCD) tetrabromobisphenol A (TBBPA) liver cell (LO2) |
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