双酚A对小鼠肝脏和肾脏细胞的氧化损伤

为探究双酚A(BPA)的氧化毒性,分别以剂量为20、40和80mg·kg~(-1)·d~(-1)的BPA对雄性昆明小鼠灌胃处理1周,并测定了小鼠体内活性氧自由基(ROS)水平、还原型谷胱甘肽(GSH)含量、丙二醛(MDA)含量和DNA-蛋白质交联系数(DPC)。与对照组相比,各BPA暴露组小鼠肝脏和肾脏细胞中的ROS生成量、MDA含量和DPC系数均升高,而GSH含量下降(P<0.05或P<0.01)。ROS生成量、GSH含量和DPC系数均显示出剂量-效应关系。研究表明,BPA可扰乱小鼠肝脏和肾脏细胞的氧化应激平衡,诱导细胞氧化损伤。

Oxidative Damage of Bisphenol A to Mouse Hepatic and Nephric Cells in Vivo

To investigate the oxidative toxicity of bisphenol A (BPA), Kunming male mice were fed daily with BPA at the doses of 20, 40 and 80
mg·kg^-1·d^-1 for one week. The reactive oxygen species (ROS) level, glutathione (GSH) content, malondialdehyde (MDA) content and DNA-protein cross-linking (DPC) coefficient of hepatocytes and nephrocytes were detected to estimate the oxidative stress in the liver and kidney of male mice. Compared with the control, ROS generation, MDA content and DPC coefficient increased while GSH content decreased in BPA exposure groups (P<0.05 or P<0.01). ROS level, GSH content and DPC coefficient all showed dose-effect relationships. It is demonstrated that BPA could induce oxidative damage by disturbing the balance between ROS and antioxidant defense system in liver and kidney of mice.