2，4’-DDT对斑马鱼生长、繁殖力及卵黄蛋白原的影响

以斑马鱼体内卵黄蛋白原（Vtg）作为雌激素污染物的生物标志物，比较研究了不同浓度2，4。DDT对成年斑马鱼生长、发育、繁殖以及体内Vtg含量的影响。结果表明，当水环境中p（2，4。DDT）为2和10μg·L-1时，斑马鱼产卵量和受精率与空白和溶剂对照组相比均显著下降（P〈0．05）。当水环境中P（2，4。DDT）为0．2、2和10μg·L-1时，斑马鱼肝脏指数与空白和溶剂对照组相比显著升高（P〈0．05）；10μg·L。2，4。DDT处理组雄性斑马鱼体内Vtg含量显著高于空白和溶剂对照组（P〈0．05），而0．2、2和10μg·L～2，4。DDT处理组雌性斑马鱼体内Vtg含量均显著高于空白和溶剂对照组（P〈0．05），且随暴露浓度的增加而增大。SDS—PAGE检测及免疫印迹分析均显示雄鱼血液内出现Vtg特异条带，表明2，4。DDT对斑马鱼具有雌激素效应。

Impacts of 2,4＇- DDT on Growth, Reproduction and Vitellogenin （Vtg） of Zebrafish.

Using zebrafish＇s vitellogenin as a biomarker of estrogenic pollutants, a comparative study was carried out to determine impacts of 2,4＇-DDT, varying in concentration, on growth, development, reproduction and vitellogenin in vi- vo. Results show that in water containing 2 and 10μg L-l of 2,4＇- DDT, the egg yield and egg fertilization rate of fish decreased significantly, as compared with the blank group （P 〈 0. 05 ）. In water containing 0. 2, 2, and 10 μg . L-1 of 2,4＇- DDT, the fish increased significantly in its hepaticosomatic index （P 〈 0. 05 ） ; A significant increase was observed in vitellogenin level of male fish treated with 10 μg. L- 1 2,4＇-DDT （P 〈 O. 05 ）, and of female fish treated with 0. 2, 2, and 10 μg . L-1 2,4＇- DDT （P 〈 0.05 ）. And the increase got more significant with the exposure increasing in concentra- tion. SDS - PAGE and Western blotting analysis revealed that a Vtg fragment appeared in the blood of male fish, which in-dicates that 2,4＇-DDT has estrogenic effects on zebrafish.