First in vitro use of the phenylphosphate carboxylase enzyme in supercritical CO2 for the selective carboxylation of phenol to 4-hydroxybenzoic acid |
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Authors: | Angela Dibenedetto Rosa Lo Noce Carlo Pastore Michele Aresta Carlo Fragale |
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Institution: | (1) Department of Chemistry, Campus Universitario, 70126 Bari, Italy |
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Abstract: | A new biotechnological synthetic approach to 4-hydroxybenzoic acid is reported. Thauera aromatica cells produce the phenylphosphate carboxylase enzyme that selectively carboxylates phenylphosphate in the para position, without any formation of the ortho isomer. Here we show that the enzyme also works in supercritical carbon dioxide, opening a new technological solution to the recovery of the products that is a key problem when aqueous media are used. The enzyme shows an activity comparable with that shown under CO2 pressure in aqueous media and does not require the presence of NaHCO3. |
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Keywords: | Carbon dioxide Supercritical fluids Thauera aromatica Phenylphosphate carboxylase 4-Hydroxybenzoic acid |
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