| 农产品中黄曲霉素B1的酶联免疫吸附分析法 |
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| 引用本文： | 李治祥,王勇.农产品中黄曲霉素B1的酶联免疫吸附分析法[J].环境化学,1993,12(4):293-298. |
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| 作者姓名： | 李治祥 王勇 |
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| 作者单位： | 农业部环境保护科学研究所，农业部环境保护科学研究所，农业部环境保护科学研究所，农业部环境保护科学研究所 天津 300191，天津 300191，天津 300191 天津市农科院，天津 300191 |
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| 基金项目： | 联合国粮农组织(FAO/UN)资助项目 |
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| 摘 要： | 使用牛血清白蛋白(BSA)与黄曲霉素B_1(AFB_1)的结合物免疫兔子获得了高特异性、高效价的抗AFB_1的抗体,用此抗体建立了测定AFB_1的酶联免疫吸附分析法(ELISA),最低检出限为1.0pg/m。应用于花生、花生酱、玉米和大豆等农产品中AFB_1分析,其回收率在81—116％范围,变异系数在1.51—10.65％之间。此法具有简单、快速和适于大批量样品分析等优点。
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| 关 键 词： | 农产品 黄曲霉素B1 ELISA |
ENZYME-LINKED IMMUNOSORBENT ASSAY FOR AFLATOXIN-B_1 IN AGRICULTURAL COMMODITIES |
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| Li Zhixiang Wang Yong Zhu Haiying Zhang Junting.ENZYME-LINKED IMMUNOSORBENT ASSAY FOR AFLATOXIN-B_1 IN AGRICULTURAL COMMODITIES[J].Environmental Chemistry,1993,12(4):293-298. |
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| Authors: | Li Zhixiang Wang Yong Zhu Haiying Zhang Junting |
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| Abstract: | High specificity, high titre antiserum to aflatoxin Bi(AFBi) has been produced by immunisation of rabbits with a bovine serum albumin-aflato-xin B1 conjugate. The antiserum has been used to develop an enzyme-linked immunosorbent assay (ELISA) with a limit of 3.0 pg/ml. The assay has been validated for application to peanut, peanut butter, bean, and corn, Analytical recoveries were between 81-116%,the coefficients of variation(CV) were between 1.51% and 10.65%.This method has many advantages over conventional methods including its technical simplicity, its analytical rapidity and its potential high sample through-put. |
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| Keywords: | agricutural commodities aflatoxin B1 ELISA |
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