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胞外聚合物物理法提取过程的优化
引用本文:葛利云,王红武,马鲁铭,李晓岩.胞外聚合物物理法提取过程的优化[J].环境化学,2006,25(6):722-725.
作者姓名:葛利云  王红武  马鲁铭  李晓岩
作者单位:1. 同济大学环境科学与工程学院,城市污染控制国家工程研究中心,上海,200092
2. 香港大学土木工程系,香港
摘    要:对物理法提取活性污泥中的胞外聚合物(EPS)进行了优化.分别考察了超声时间、振摇时间、离心速度和时间以及加热时间对EPS的松散附着(LB)和紧密粘附(TB)提取的影响,得出污泥EPS物理法提取的优化方案.

关 键 词:活性污泥  胞外聚合物  提取  DNA
收稿时间:2005-11-16
修稿时间:2005-11-16

OPTIMIZATION OF EXTRACTION PROCESS OF EXTRACELLULAR POLYMERIC SUBSTANCES BY PHYSICS METHOD
GE Li-yun,WANG Hong-wu,MA Lu-ming,LI Xiao-yan.OPTIMIZATION OF EXTRACTION PROCESS OF EXTRACELLULAR POLYMERIC SUBSTANCES BY PHYSICS METHOD[J].Environmental Chemistry,2006,25(6):722-725.
Authors:GE Li-yun  WANG Hong-wu  MA Lu-ming  LI Xiao-yan
Institution:1 The State Engineering Research Center for Municipal Pollution Control, College of Environmental Science and Engineering Tongji University, Shanghai, 200092, China; 2 Department of Civil Engineering, Hongkong University, Hongkong, China
Abstract:The extraction of extracellular polymeric substances(EPS) is one key of quantitative analysis of EPS in activated sludge.Optimization of EPS extraction by physics method in activated sludge was investigated for a good extraction process.Extracellular polymeric substances consist of loosely bound EPS(LB) and tightly bound EPS(TB).The influence of ultrasonic time,centrifuging speed and time,shaking time and heating time on LB and TB extraction was studied here,respectively.At last,an optimization scheme of EPS extraction by physics method was generated.
Keywords:activated sludge  extracellular polymeric substances  extraction  DNA  
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