| 白腐菌对十溴联苯醚的酶促降解研究 |
| |
| 引用本文： | 熊士昌,尹华,何宝燕,彭辉,龙焰,叶锦韶,张娜,彭素芬.白腐菌对十溴联苯醚的酶促降解研究[J].环境化学,2012,31(5):615-619. |
| |
| 作者姓名： | 熊士昌 尹华 何宝燕 彭辉 龙焰 叶锦韶 张娜 彭素芬 |
| |
| 作者单位： | 暨南大学环境工程系,广东省高校水土环境毒害性污染物防治与生物修复重点实验室,广州510632 |
| |
| 基金项目： | 国家自然科学基金委-广东省联合基金重点项目，国家自然科学基金项目，中央高校基本科研业务费专项资金资助项目 |
| |
| 摘 要： | 考察了白腐菌菌体、胞外酶、胞内酶对十溴联苯醚(BDE-209)的降解性能,结果表明,白腐菌菌体、胞外酶、胞内酶对浓度为1 mg.L-1的BDE-209的降解率分别为60.17%、54.14%、22.32%,白腐菌对BDE-209的降解主要由胞外酶完成.进一步考察了温度、pH、BDE-209浓度对白腐菌胞外酶降解BDE-209的影响,结果显示,胞外酶降解BDE-209的最适条件为温度30℃、pH 4—5、BDE-209浓度1 mg.L-1.白腐菌胞外酶降解BDE-209前后红外光谱测定结果证实,BDE-209降解过程与Br—C、CH2—O—CH2等基团有关.降解后体系GC-MS谱图显示,BDE-209降解过程中存在脱Br作用.
|
| 关 键 词： | BDE-209 胞外酶 降解 |
Biodegradation of BDE-209 by enzyme obtained from phanerochaete chrysosporium |
| |
| XIONG Shichang,YIN Hua,HE Baoyan,PENG Hui,LONG Yan,YE Jinshao,ZHANG Na,PENG Sufen.Biodegradation of BDE-209 by enzyme obtained from phanerochaete chrysosporium[J].Environmental Chemistry,2012,31(5):615-619. |
| |
| Authors: | XIONG Shichang YIN Hua HE Baoyan PENG Hui LONG Yan YE Jinshao ZHANG Na PENG Sufen |
| |
| Institution: | (Department of Environmental Engineering,Jinan University,Key Laboratory of Water/soil Toxic Pollutants Control and Bioremediation of Guangdong Higher Education Institutes,Jinan University,Guangzhou,510632,China) |
| |
| Abstract: | Degradation of BDE-209 by the cell,extracellular and intracellular enzymes of P.chrysosporium was investigated,and after 7 days 60.17%,54.14% and 22.32% BDE-209 was degraded respectively at an initial BDE-209 concentration of 1 mg·L-1,which demonstrated that BDE-209 was mainly degraded by extracellular enzymes.The effect of temperature,pH,and BDE-209 concentration on the degradation of BDE-209 was further studied.The results indicated that the optimal conditions of BDE-209 degradation were as follow: temperature 30 ℃,pH 4—5,BDE-209 concentration 1 mg·L-1.Infrared spectroscopy analysis showed that the degradation of BDE-209 was related to Br—C、CH2—O—CH2.The GC-MS chromatogram revealed that deprivation of Br occured during the degradation of BDE-209. |
| |
| Keywords: | BDE-209 extracellular enzymes degradation |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
