硝基化合物的高效液相色谱(HPLC)分析

硝基化合物是炸药废水的主要成分,大部分硝基化合物具有较高的毒性.本文对奥克托今、黑索今、1,3,5-三硝基苯、1,3-二硝基苯、硝基苯、2,4,6-三硝基甲苯、2-氨基-4,6-二硝基甲苯、2,4-二硝基甲苯共8种硝基化合物的紫外光谱和液相色谱分离条件进行了研究,其最佳检测波长分别为228 nm、227 nm、227 nm、237 nm、272 nm、230 nm、226 nm、244 nm.本文建立了8种硝基化合物的高效液相色谱测定方法,色谱条件为:色谱柱为ZORBAX SB-C18(3.0 mm×250 mm,5μm),检测器为紫外检测器,流动相为甲醇-水(50∶50),流速为0.5 mL.min-1.水中8种硝基化合物可以在13 min内得到较好的分离,检出限均≤0.8 ng,回收率大于95%.

Studies on the HPLC analysis conditions of nitryl compounds

Nitro compounds are the main components of explosive wastewater.Most nitro compounds are poisonous.This paper studied the ultraviolet spectra and HPLC separation conditions of eight nitro compounds,including HMX,RDX,1,3,5-trinitrobenze,1,3-dinitrobenzeze,Nitrobenzene,TNT,2-Amino-4,6-dinitrotoluene and 2,4-dinitrotoluene.Their optimal determination wavelengths are 228 nm,227 nm,227 nm,237 nm,272 nm,230 nm,226 nm,244 nm respectively.The HPLC method is employed with ZORBAX SB-C18 column(3.0 mm×250 mm,5 μm) and a UV detector.The mobile phase was methanol mingled with water(Vmethanol∶Vwater=50∶50) at a flow rate of 0.5 mL·min-1.The eight nitro compounds can be separated well in 13 min,the detection limits are less than 0.8 ng and the recoveries are more than 95%.