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超高效液相色谱串联质谱法检测鱼体中的全氟化合物
引用本文:孔德洋,王懿,王娜,单正军,谭立超.超高效液相色谱串联质谱法检测鱼体中的全氟化合物[J].环境化学,2012,31(5):743-747.
作者姓名:孔德洋  王懿  王娜  单正军  谭立超
作者单位:环境保护部南京环境科学研究所,南京,210042
基金项目:环保公益性行业科研专项
摘    要:采用超高效液相色谱-串联质谱联用法(UPLC-ESI-MS/MS),建立了检测1种贝类和2种鱼类的肌肉组织中11种全氟化合物(PFCs)的分析方法.采用碱液消解做为样品前处理法,选Carbon/NH2双层SPE小柱做为净化小柱,并以ACQUITY UPLC BEH C18为分析柱,甲醇和2 mmol.L-15%甲醇乙酸铵溶液为梯度淋洗液.所选定的11种全氟化合物在6 min内就可以达到良好分离,外标法定量.平均回收率在72.1%—93.6%之间,相对标准偏差在0.6%—9.5%之间,实际检出限在3.4—26.7 pg.g-1.

关 键 词:超高效液相色谱串联质谱法  全氟化合物  鱼类  肌肉  固相萃取

Ultra performance liquid chromatography-tandem mass spectrometry for determination of perfluorinated compounds in biological samples of fish
KONG Deyang,WANG YiWANG Na,SHAN Zhengjun,TAN Lichao.Ultra performance liquid chromatography-tandem mass spectrometry for determination of perfluorinated compounds in biological samples of fish[J].Environmental Chemistry,2012,31(5):743-747.
Authors:KONG Deyang  WANG YiWANG Na  SHAN Zhengjun  TAN Lichao
Institution:(Nanjing Institute of Environmental Science,Ministry of Environmental Protection of the People′s Republic of China,Nanjing,210042,China)
Abstract:A method for the determination of 11 perfluorinated compounds(PFCs) in biological muscle samples of two kinds of fish and one kind of shellfish by ultra performance liquid chromatography-tandem mass spectrometry(UPLC-ESI-MS/MS)was developed.The perfluorinated compounds have been analyzed by the method which recommended using alkaline digestion as a sample preparation method,Carbon/NH2 double SPE cartridges as the purification cartridge,the column of ACQUITY UPLC BEH C18 as the analyzing column,and methanol and 2 mmol·L-1 5% methanol ammonium acetate solvent as the binary gradient eluent.The 11 PFCs could be seperated in 6 min,and quantified by the external standard method.The average recoveries of 11 PFCs were determined to be were in the range of 72.1%—93.6%,with the relative standard deviations(RSD) of 0.6%—9.5%.The limits of detection(LODs) were in the range of 3.4—26.7 pg·g-1.
Keywords:ultra performance liquid chromatography tandem mass spectrometry  perfluorinated compounds  fish  muacle  SPE  
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