| 应用紫外及荧光光谱法研究三种醛类污染物对牛腺DNA的损伤作用 |
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| 引用本文： | 袭著革,杨丹凤.应用紫外及荧光光谱法研究三种醛类污染物对牛腺DNA的损伤作用[J].环境化学,1999,18(5):453-457. |
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| 作者姓名： | 袭著革 杨丹凤 |
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| 作者单位： | [1]南开大学环境科学系 [2]军事医学科学院卫生学环境医学研究所 |
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| 基金项目： | 国家教李博士点专项基金(No.BBA24A) |
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| 摘 要： | 应用紫外光谱移动法和溴乙锭荧光法对三种醛类化合物与牛腺ＤＮＡ的加合以及它们引起的ＤＮＡ－ＤＮＡ交联进行了检测。结果表明，甲醛和乙醛能够与ＤＮＡ形成共价加合物，与四种脱氧核苷加合顺序分别为ｄＧ〉ｄＣ〉ｄＡ〉ｄＴ和ｄＡ〉ｄＧ〉ｄＣ〉ｄＴ，丙烯醛与ＤＮＡ的加合不能用紫外光谱移动法测定。甲醛能够显著地引起ＤＮＡ－ＤＮＡ的交联；乙醛也有这种作用，但不显著；丙烯醛的交联作用未测出，可能是由于羰基碳正电性较弱，
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| 关 键 词： | 醛类污染物 DNA加合 DNA交联 损伤作用 |
STUDIES ON THE DAMAGE OF CALF THYMUS DNA INDUCED BY 3 KINDS OF ALDEHYDE POLLUTANTS USING ULTRAVIOLET AND FLUORESCENCE SPECTRUM |
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| Xi Zhuge Dai Shugui Zhang Lin.STUDIES ON THE DAMAGE OF CALF THYMUS DNA INDUCED BY 3 KINDS OF ALDEHYDE POLLUTANTS USING ULTRAVIOLET AND FLUORESCENCE SPECTRUM[J].Environmental Chemistry,1999,18(5):453-457. |
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| Authors: | Xi Zhuge Dai Shugui Zhang Lin |
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| Abstract: | Two kinds of DNA damage-DNA adducts and DNA-DNA strand crosslink caused by fonnaldehyde , acetaldehyde and acrolein were determined by ultraviolet and fluorescence spectrum. The results showed that fonnaldehyde and acetaldehyde could react with DNA and deoxynucleosides to form covalent adducts. The reaction sequence is dG > dC > dA > dT and dA > dG > dC > dT respectively . The reaction of acrolein with DNA could not determined by this method because of its UV adsorption peak covering that of DNA. Fonnaldehyde and acetaldehyde can cause DNA-DNA strand crosslink. The cross-linked effect of acrolein was not detected. |
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| Keywords: | aldehyde pollutants DNA adducts DNA-DNA strand crossling |
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