| 以壳聚糖微球为载体的固定化乙酰胆碱酯酶的基本性质 |
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| 引用本文： | 张爱静,孟范平,杨菲菲.以壳聚糖微球为载体的固定化乙酰胆碱酯酶的基本性质[J].环境化学,2011,30(6):1068-1074. |
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| 作者姓名： | 张爱静 孟范平 杨菲菲 |
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| 作者单位： | 中国海洋大学海洋环境与生态教育部重点实验室,青岛,266100 |
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| 基金项目： | 国家高技术研究发展计划项目(2001AA635130)资助 |
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| 摘 要： | 为探讨以壳聚糖微球为载体制备的固定化乙酰胆碱酯酶（AChE）用于监测海水有机磷农药的可行性,研究了固定化酶在海水中的基本性质,如环境适应性、动力学性质、稳定性、对农药响应规律等.结果表明,与溶液酶相比,固定化酶对海水温度、盐度、pH值等因素的适应能力提高;固定化酶分子构象的改变增加了酶与底物之间的空间障碍,米氏常数增加...
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| 关 键 词： | 壳聚糖 固定化 乙酰胆碱酯酶 性质 海水 |
CHARACTERIZATION OF ACETYLCHOLINESTERASE IMMOBILIZED ON CHITOSAN MICROSPHERES |
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| ZHANG Aijing MENG Fanping YANG Feifei.CHARACTERIZATION OF ACETYLCHOLINESTERASE IMMOBILIZED ON CHITOSAN MICROSPHERES[J].Environmental Chemistry,2011,30(6):1068-1074. |
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| Authors: | ZHANG Aijing MENG Fanping YANG Feifei |
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| Institution: | ZHANG Aijing MENG Fanping YANG Feifei(Key Laboratory of Marine Environment and Ecology,Ministry of Education,Ocean University of China,Qingdao,266100,China) |
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| Abstract: | In order to evaluate the feasibility of using immobilized acetylcholinesterase(AChE) on chitosan microspheres to monitor organophosphorus pesticides(OPs) in seawater,basic properties of the immobilized enzyme were investigated,such as environmental adaptability,dynamic property,stability,response characteristics to OPs.The results indicated that the immobilized enzyme was more adaptive to the temperature,salinity,pH of seawater compared with the solution enzyme.The Michaelis constant of the immobilized AChE... |
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| Keywords: | chitosan immobilization acetylcholinesterase(AChE) property seawater |
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