| 2-硝基芴DNA加合物的研究 |
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| 引用本文： | 刘淑芬,蒋湘宁,徐晓白.2-硝基芴DNA加合物的研究[J].环境化学,1996,15(3):240-247. |
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| 作者姓名： | 刘淑芬 蒋湘宁 徐晓白 |
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| 作者单位： | 中国科学院生态环境研究中心!北京100085 |
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| 摘 要： | 2-硝基芴直接与小牛胸腺DNA反应(in vitro),用~(32)P后标记方法能够测定出有多种DNA加合物生成.将定量的2-硝基芴一次注射入大鼠(SD)和经过Aroclor诱导的大鼠腹腔内,24h后取其肝、肾、肺、脾、血等诸器官,用P_1加强灵敏度的~(32)P后标记方法测定各组织中的DNA加合物.其结果是:在诱导和非诱导的大鼠体内各器官中均发现有DNA加合物的存在,Aroclor诱导对DNA加合物的生成有明显的促进作用.在非诱导的大鼠体内肝脏和血中,分别测出与体外反应相对应的多个DNA加合物,证明2-硝基芴有直接损伤生物体内DNA,形成DNA加合物的能力.
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| 关 键 词： | DNA加合物 ~(32)P后标记 硝基多环芳烃 2-硝基芴 |
INVESTIGATION OF DNA-2-NF ADDUCT BY NUCLEASE P_1 MEDIATED ~(32)P-POSTLABELING IN VITRO AND IN VIVO |
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| Liu Shufen Jiang Xiangning Xu Xiaobai.INVESTIGATION OF DNA-2-NF ADDUCT BY NUCLEASE P_1 MEDIATED ~(32)P-POSTLABELING IN VITRO AND IN VIVO[J].Environmental Chemistry,1996,15(3):240-247. |
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| Authors: | Liu Shufen Jiang Xiangning Xu Xiaobai |
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| Abstract: | DNA-2-NF adducts were analyzed in biosamples reacted calf thymus DNA with 2-NF (in vitro) and in organs of rat treated with 2-NF or Aroclor and 2-NF by P,-32P-post-labelling. Results showed that some DNA-2-NF adducts were found in vitro and vivo, Rf of some DNA-2-NF adduct in vitro in liver and blood of rat treated with 2-NF were same, RAL of DNA adduct in various organs was extended with 2-NF and Aroclor, Extend of DNA adducts formation in various organs of rat treated with 2-NF was liver > kidney >lung>spleen. |
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| Keywords: | DNA adduct 32P-postlabelling N-PAH 2-NF |
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