| 乌蕨孢子繁殖技术的研究 |
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| 引用本文： | 张国珍,李策宏,谷海燕,余道平.乌蕨孢子繁殖技术的研究[J].资源开发与市场,2010,26(12). |
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| 作者姓名： | 张国珍 李策宏 谷海燕 余道平 |
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| 作者单位： | 四川省自然资源科学研究院;峨眉山生物资源实验站; |
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| 基金项目： | 四川省财政厅基本科研业务费，四川省科技条件平台项目--四川省植物资源共享平台建设项目共同资助 |
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| 摘 要： | 研究了乌蕨(Stenoloma chusanum(L.)Ching)孢子无菌繁殖和常规繁殖方法。结果表明,乌蕨孢子在1/4MS培养基培养时的萌发率最高,达85.3%;在1/2MS培养基中的配子体成苗率最高,达85.9%;1/2MS培养基是培养优质孢子体苗的最佳培养基;河沙与草炭土体积比为1∶1的混合基质是播种孢子的理想基质,孢子萌发率和配子体成苗率分别达到82.6%和73.4%;无菌繁殖和常规繁殖所得的幼孢子体苗移栽成活率均达90%以上。
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| 关 键 词： | 乌蕨 孢子 无菌培养 常规繁殖 |
Study on Spore Propagation of Stenoloma chusanum (L.) Ching |
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| ZHANG Guo-zhen,LI Ce-hong,GU Hai-yan,YU Dao-ping.Study on Spore Propagation of Stenoloma chusanum (L.) Ching[J].Resource Development & Market,2010,26(12). |
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| Authors: | ZHANG Guo-zhen LI Ce-hong GU Hai-yan YU Dao-ping |
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| Institution: | ZHANG Guo-zhen1,2,LI Ce-hong1,GU Hai-yan1,YU Dao-ping1,2(1.Sichuan Academy of Natural Resource Sciences,Chengdu 610015,China,2.Mountain Emei Biotic Resource Experimental Station,Emei 614201,China) |
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| Abstract: | The spore sterile culture and routine propagation of Stenoloma chusanum(L.) Ching were studied.The results showed that the maximum germination rate was obtained in 1/4MS medium,which reached 85.3%;the highest rate of gametophyte to sporophyte was achieved in 1/2MS medium,which reached 85.9%;1/2MS medium was the best for cultivating high quality sporophyte.The mixed soil which the sand and the turf blend in the volume ratio of 1 to 1 was the optimal growing medium for spore routine propagation,in which the r... |
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| Keywords: | Stenoloma chusanum(L )Ching spore sterile culture routine propagation |
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