| 16S rRNA基因技术在环境科学领域中的应用 |
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| 引用本文： | 乐毅全,顾国维.16S rRNA基因技术在环境科学领域中的应用[J].四川环境,2003,22(6):1-5. |
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| 作者姓名： | 乐毅全 顾国维 |
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| 作者单位： | 同济大学污染控制与资源化国家重点实验室,上海,200092 |
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| 摘 要： | 随着分子生物学的发展，16S rRNA基因技术被逐渐应用到环境科学领域中。目前在环境保护和治理中，该技术主要被用于鉴定污染物的生物降解菌和分析环境样品中的微生物群落多样性，由于它不必将微生物培养分离出来，也就避免了在培养过程中可能出现的微生物去失的情况。本文对16S rRNA基因技术及其在环境科学领域中的应用现状和发展作了一简要介绍，并对16S rRNA基因技术存在的不足进行了讨论。
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| 关 键 词： | 16S rRNA基因序列 DNA扩增 多样性分析 |
| 文章编号： | 1001-3644(2003)06-0001-04 |
| 修稿时间： | 2003年4月10日 |
The Application of 16S rRNA Gene Technology on Environmental Sciences |
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| LE Yi-quan,GU Guo-weiTongji University,Shanghai ,China.The Application of 16S rRNA Gene Technology on Environmental Sciences[J].Sichuan Environment,2003,22(6):1-5. |
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| Authors: | LE Yi-quan GU Guo-weiTongji University Shanghai China |
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| Institution: | LE Yi-quan,GU Guo-weiTongji University,Shanghai 200092,China) |
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| Abstract: | With the development of molecular biology, the 16S rRNA gene technique has been gradually used in environmental sciences. Now, this technique has been mainly applied to identify the pollutant-biodegradation bacteria and to analyse the diversity of microorganism community in environmental samples. Because it is unnecessary to culture the microorganisms by traditional methods, it can avoid the situation of losing the microorganisms during the culture. In this paper, it briefly introduces that the development and the use of 16S rRNA gene technique on environmental sciences. The disadvantage of 16S rRNA gene technique is also discussed. |
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| Keywords: | 16S rRNA gene sequence DNA amplification diversity analysis |
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