The metabolic pathway of 4?Hz mechanical vibration-induced effect on snail heart muscle contractility |
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Authors: | Erna Dadasyan Gayane Ayrapetyan Naira Baghdasaryan Yerazik Mikayelyan Sinerik Ayrapetyan |
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Institution: | 1. UNESCO Chair-Life Science International Postgraduate Educational Center, 31 Acharyan St., 0040, Yerevan, Armenia
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Abstract: | It was shown that the 4 Hz 30 dB mechanical vibration (MV) of physiological solution (PS) had modulation effect on snail heart
contractility. However, the nature of metabolic pathway of MV-treated PS-induced effect is not clear yet. It was suggested
that the MV-induced modulation of water molecules dissociation leads to the variation of reactive oxygen species’ level in
cell bathing medium, which could serve as a messenger for switching on the metabolic pathway(s) responsible for modulation
of muscle contractility. The aim of present work was to check this hypothesis and to elucidate the metabolic pathway through
which the effect of MV-treated PS on heart contractility was realized. For this purpose, the effect of MV on heat fusion periods
(HFP) and H2O2 content in PS, as well as on heart contractility, 45Ca2+ efflux, intracellular levels of cGMP and cAMP, muscle hydration, and ouabain binding were studied. It was shown that MV treatment
of PS increased the HFP-(21.33 ± 4%) and decreased the H2O2 content-(5 ± 0.9%). The intracardiac perfusion by MV-treated PS increased the amplitudes of heart contractility, which was
accompanied by the increasing of 45Ca2+ efflux (252.4 ± 16%), elevation of cGMP’s level (42.05 ± 7%), decreasing of cAMP’s level (82.67 ± 7%), increasing of the
tissue hydration (18.64 ± 3%), and increasing of the number of ouabain binding sides (25 ± 4%). It was suggested that MV-induced
increasing of heart muscle contraction amplitudes is due to the decreases of H2O2 content in the medium, which leads to the elevation of heart muscle contractility in result of activation of cGMP-dependent
Na+/Ca2+ exchange in forward regime. |
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