Effects of in vitro exposure to GSM900 electromagnetic field on human erythrocytes

High frequency electromagnetic fields (EMF) are widely used for transmitting of radio and TV signals, in wireless communications, etc. A huge number of people are exposed so the possible risk to human health from telecommunication technologies could be significant even if biological effects are slight. The study of the biological effects of RF EM radiation could contribute to better understanding of the possible health hazards. The levels of released hemoglobin serve as an indicator of hemolysis, caused by increased membrane fragility. This study was designed to investigate the alterations in hemoglobin release after in vitro exposure of human erythrocytes to GSM900 electromagnetic field. Erythrocyte suspensions with two different cell concentrations (hematocrit 20% and 40%) were exposed to EM radiation from GSM mobile phone (carrier frequency 902 MHz, 2 W output power in pulse) for 20 min in two different positions in relation to telephone antenna: Position 1 is in the centre of the major lobe of the azimuth antenna pattern and Position 2 is between major and back lobes. Alterations in hemolysis were registered 0, 10, 20, 30, 40, 50 and 60 min after the treatment. Hemolysis was determined by measuring the absorbance of hemoglobin at 413 nm in the supernatant obtained by centrifuging the suspensions. Hemolysis was expressed as hemoglobin concentration. Our data indicated decrease in the hemoglobin level in irradiated suspensions. The GSM900 EMF exposure probably stabilized erythrocyte membrane and caused reduction in the hemolysis depending on the EMF parameters, on the suspension water content (hematocrit) and on the time elapsed after irradiation.