Comparison of different pre-treatment methods for enriching hydrogen-producing bacteria from intertidal sludge |
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| Authors: | Jinling Cai Guangce Wang |
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| Institution: | 1. College of Chemical Engineering and Materials Science, Tianjin University of Science &2. Technology, Tianjin Key Laboratory of Marine Resources and Chemistry, Tianjin, China;3. Tianjin International, Joint Academy of Biomedicine, Tianjin, China;4. College of Biotechnology, Tianjin University of Science &5. Technology, Tianjin, China;6. Institute of Oceanology, Chinese Academy of Sciences, Qingdao, Shandong, China |
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| Abstract: | Batch tests of cultivations were conducted to analyze influences of pre-treatment methods (heat–shock, acid, and alkaline) on hydrogen production. The hydrogen yields of the pre-treated samples were significantly higher than those without pre-treatment. Heat–shock pre-treatment yielded maximum hydrogen (75.5 ± 4.0 mL/g sucroseremoved), and had the highest sucrose removal efficiency (81.20 ± 1.23%). Heat–shock pre-treatment produced more ethanol and acetate (80.44 ± 0.55% of the total soluble metabolites), which belonged to ethanol-type fermentation. Denaturing gradient gel electrophoresis (DGGE) profiles showed that pre-treatment methods affected the composition of microbial communities. The results showed that pre-treatment methods play a key role in the hydrogen production process of marine hydrogen-producing microflora. |
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| Keywords: | Biological hydrogen production fermentation PCR-DGGE pre-treatment seawater |
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