Detection of potential harmful algal bloom-causing microalgae from freshwater prawn farms in Central Luzon,Philippines, for bloom monitoring and prediction

Prawn aquaculture industry is one of the developing economic activities in the Philippines. Generally, a wide range of microalgae typically grow and proliferate in many aquaculture ponds. However, certain species of microalgae have the ability to form harmful algal blooms (HABs) which often bring damaging consequences to the aquaculture industry, food safety, and the environment. The study aims to identify and characterize the composition of microalgae (particularly Cyanophyta) that are capable of forming HABs in selected freshwater prawn culture ponds in Central Luzon, Philippines, using morphological, ultrastructural, and molecular characterizations. From water samples collected in ten selected ponds across Central Luzon (Bulacan, Nueva Ecija, Pampanga, Tarlac, and Zambales), potential HAB formers such as Oscillatoria agardhii, O. princeps, Microcystis aeruginosa, and M. wesenbergii were observed to have occurred mostly, while Anabaena circinalis was only observed in one site. Both morphological and ultrastructural methods brought upon some challenges and limitations while molecular methods using 16S rRNA gene and phylogenetic analysis which were optimized in this study offered better and more efficient ways of identification and were helpful in resolving genus-level and species-level relationships. The influence of physicochemical properties of pond water, especially nutrient levels (nitrate, nitrite, and orthophosphate) on the occurrence of these cyanobacteria was also analyzed. As a pioneering study on freshwater HABs in aquaculture ponds in the country, results of the study can provide information to improve the knowledge in bloom occurrence and prediction, and to develop freshwater HAB prevention and control methods.