西昌高原鳅人工繁殖技术研究

对西昌高原鳅(Triplophysa xichangensis)人工繁殖中的催产药物及其剂量、受精方式、孵化方法和仔鱼水花培育方法等一系列关键技术进行了详细研究。结果表明,对性腺发育至Ⅳ+~Ⅳ++期的雌鱼分二次注射LRH-A2 0.1μg/g(鱼体重)和HCG 20 IU/g(鱼体重),雄鱼剂量减半,注射量0.1 mL,能很好地促进雌雄鱼发情并产卵排精,在水温19±0.5℃时的效应时间为30.5~31 h;在一个特制的塑料盆中完成了亲鱼培育、打针催产、发情、自然产卵受精、孵化以及仔鱼培育等全部人工繁殖过程,受精率、孵化率和仔鱼水花培育20 d的成活率平均分别为97.2%、85%和85%。本研究成果可应用于西昌高原鳅规模化繁育,对长江上游鱼类生物多样性保护有着积极意义。

STUDIES ON ARTIFICIAL PROPAGATION TECHNIQUES OF TRIPLOPHYSA XICHANGENSIS

In this study we carried out a series of experiments on every key technologies of the artificial propagation of Triplophysa xichangensis, including oxytocin drugs and dosage, the insemination method, and the larval hatch and culture method. The results showed that, when the gonad of the female fish developed to the Ⅳ+-Ⅳ++ period, twice injection was given with 0.1 ml hormone containing LRH-A at a dose of 0.1 μg/g fish and HCG at a dose of 20 IU/g fish, while the doses were halved for male fish, it could preferably promote brood stock estrus and spawn. The response and effective time of the hormone was 30.5-31h at a water temperature of 19±0.5℃. All the artificial propagation processes of the fish, including the intensive cultivation of brood stock, hormone injection and natural spawning, hatching of fertilized eggs, cultivation of larvae fish, were carried out in a special plastic basin. The fertilization rate and hatching rate was 97.2% and 85%, respectively, and the survival rate of the 20d larvae fish was 85%. This research can be applied in large-scale breeding of Triplophysa xichangensis, and has positive significance for the protection of fish biodiversity in the upper Yangtze River.