| 鄱阳湖南矶山自然保护区鲤、鲫的随机扩增多态DNA分析 |
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| 引用本文： | 胡茂林,吴志强,常剑波.鄱阳湖南矶山自然保护区鲤、鲫的随机扩增多态DNA分析[J].长江流域资源与环境,2007,16(3):314-317. |
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| 作者姓名： | 胡茂林 吴志强 常剑波 |
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| 作者单位： | 南昌大学教育部鄱阳湖湖泊生态与生物资源利用实验室,江西,南昌,330047;中国科学院水生生物研究所,湖北,武汉,330047 |
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| 基金项目： | 江西省科技厅与中国科学院科技合作项目，江西省科技厅重大科技招标项目(九江观音塘)，南昌市科技局成果推广项目 |
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| 摘 要： | 利用RAPD(随机扩增多态)标记分析湖内鲤、鲫群体遗传多样性,从40个随机引物中各筛选出8个引物适合鲤、鲫群体RAPD扩增.在鲤群体中,共检测出60条带,其中多态性带42条,多态位点比率为70.00%;而在鲫群体中,共检测出61条带,其中多态性带40条,多态位点比率为65.57%.用POPGENE软件分析实验数据,结果显示:湖内鲤群体的遗传多样性水平(He=0.230 1,H0=0.391 0)和鲫群体的遗传多样性水平(He=0.218 6,H0=0.375 8)都较高,都有较大的遗传变异.而在鲤、鲫群体内,雌性群体与雄性群体相比较,其各自的遗传多样性很接近.
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| 关 键 词： | 鄱阳湖 南矶山自然保护区 鲤 鲫 RAPD |
| 文章编号： | 1004-8227（2006）03-0314-04 |
| 收稿时间： | 2006-03-07 |
| 修稿时间： | 2006-06-16 |
RAPD ANALYSIS OF GENETIC DIVERSITY OF CYPRINUS CARPIO AND CARASSIUS AURATUS IN NANJISHAN NATURE RESERVE IN POYANGHU LAKE |
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| HU Mao-lin,WU Zhi-qiang,CHANG Jian-bo.RAPD ANALYSIS OF GENETIC DIVERSITY OF CYPRINUS CARPIO AND CARASSIUS AURATUS IN NANJISHAN NATURE RESERVE IN POYANGHU LAKE[J].Resources and Environment in the Yangtza Basin,2007,16(3):314-317. |
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| Authors: | HU Mao-lin WU Zhi-qiang CHANG Jian-bo |
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| Institution: | 1. Key Laboratory of Poyang Lake Ecology and Bio-resource Utilization of Ministry of Education Nanchang University, Nanchang 330047, China; 2. Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China |
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| Abstract: | The colonial genetic variation in Cyprinus carpio and Carassius auratus was analyzed with RAPD technique.Eight primers were screened out from 40 random primers on the genomic DNA in Cyprinus carpio.A total of 60 bands were generated,of which polymorphic bands were 42,with the percentage of polymorphism being 70.00%.Using the 8 primers for genomic DNA of Carassius auratus,61 bands were generated,of which 40 were polymorphic,with the percentage of polymorphism being 65.57%.As analyzed by software POPGENE,the colonial genetic diversity was both high in Cyprinus carpio(He=0.230 1,H0=0.391 0) and Carassius auratus(He=0.218 6,H0=0.375 8).However,in the population of Cyprinus carpio and Carassius auratus,the colonial genetic diversity was very similar in females and males. |
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| Keywords: | RAPD |
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