| N-乙烯基吡咯烷酮降解菌的筛选、降解特性及其竹炭固定化 |
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| 引用本文： | 郑菲菲, 胡江, 辛华东, 陈立伟, 蔡天明. N-乙烯基吡咯烷酮降解菌的筛选、降解特性及其竹炭固定化[J]. 环境工程学报, 2016, 10(10): 5593-5599. doi: 10.12030/j.cjee.201505199 |
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| 作者姓名： | 郑菲菲 胡江 辛华东 陈立伟 蔡天明 |
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| 作者单位： | 1.南京农业大学资源与环境科学学院环境工程系, 南京 210095 |
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| 基金项目： | 江苏省太湖水环境综合治理科研课题（TH2014210，TH2013210） |
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| 摘 要： | 从活性污泥中分离筛选得到1株N-乙烯基吡咯烷酮（NVP）高效降解菌株ZF1，根据菌株ZF1的形态特征、生理生化特性和16S rRNA基因序列同源性分析，将其初步鉴定为产脲节杆菌（Arthrobacter ureafaciens）。菌株ZF1能以NVP为唯一碳、氮源进行生长，并在60 h内完全降解200 mg·L-1的NVP。菌株ZF1降解NVP的最适环境条件为温度30℃，初始pH 7.0，NaCl浓度7 g·L-1。为了更好地实现其在实际废水中的应用，将菌株ZF1固定到竹炭上，扫描电镜观察表明ZF1能很好的附着在竹炭表面，且固定菌对NVP的降解效率明显高于游离菌。游离菌和固定菌对NVP的降解均符合一级动力学模型。重复利用5次后，固定菌对NVP的降解率仍能达到98%。
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| 关 键 词： | N-乙烯基吡咯烷酮 分离鉴定 Arthrobacter ureafaciens 降解特性 竹炭 固定化 |
| 收稿时间： | 2015-06-19 |
Isolation and degradation characteristics of a N-vinylpyrrolidone-degrading strain ZF1 and its immobilization on bamboo-based carbon |
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| ZHENG Feifei, HU Jiang, XIN Huadong, CHEN Liwei, CAI Tianming. Isolation and degradation characteristics of a N-vinylpyrrolidone-degrading strain ZF1 and its immobilization on bamboo-based carbon[J]. Chinese Journal of Environmental Engineering, 2016, 10(10): 5593-5599. doi: 10.12030/j.cjee.201505199 |
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| Authors: | ZHENG Feifei HU Jiang XIN Huadong CHEN Liwei CAI Tianming |
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| Affiliation: | 1.Department of Environmental Engineering, College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing 210095, China |
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| Abstract: | N-vinylpyrrolidone (NVP) is a common raw material widely used in many industries and belongs to the nitrogen heterocyclic compounds (NHCs).Biological degradation was demonstrated as the most cost effective and environmentally friendly way to remove nitrogen heterocyclic pollutants from the environment.In the current study,a highly effective NVP-degrading bacteria,designated ZF1,was isolated from activated sludge.Based on its morphological,physiological,and biochemical properties and 16S rRNA gene sequence,the strain was identified as Arthrobacter ureafaciens.Strain ZF1 could use NVP as its sole carbon and nitrogen sources,degrading 200 mg·L-1 NVP to a nondetectable level within 60 h.The optimal environmental conditions for the degradation of NVP were 30℃,initial pH of 7.0,and NaCl of 7 g·L-1.For its application in real wastewater treatment,strain ZF1 was immobilized onto bamboo-based carbon to enhance the NVP degradation efficiency.SEM observation indicated that strain ZF1 cells were attached well on the surface and pores of the bamboo-based carbon.The degradation kinetics of NVP by free and immobilized cells followed the first-order model.After it was reused 5 times,NVP degradation efficiency was maintained at 98%. |
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| Keywords: | N-vinylpyrrolidone isolation and identification Arthrobacter ureafaciens degradation characteristics bamboo-based carbon immobilization |
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