| 批次培养条件下三角褐指藻对不同磷胁迫压力及恢复的响应 |
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| 引用本文： | 李文娇,冯剑丰,朱琳,乔银环. 批次培养条件下三角褐指藻对不同磷胁迫压力及恢复的响应[J]. 海洋环境科学, 2016, 35(6): 894-900. DOI: 10.13634/j.cnki.mes.2016.06.015 |
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| 作者姓名： | 李文娇 冯剑丰 朱琳 乔银环 |
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| 作者单位： | 南开大学 环境科学与工程学院, 污染过程与环境基准教育部重点实验室, 天津市城市生态环境修复与污染防治重点实验室, 天津 300071 |
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| 基金项目： | 天津市自然科学基金资助项目（15JCYBJC22800） |
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| 摘 要： | 采用批次培养，设置5个PO4-P浓度（0 μmol/L，0.566 μmol/L，2.26 μmol/L，9.05 μmol/L，36.2 μmol/L）进行实验，并从第9 d开始进行2 d恢复实验，研究三角褐指藻在不同磷胁迫压力及恢复下的响应。测定参数包括PS Ⅱ的最大光能转化效率（Fv/Fm）、PS Ⅱ的实际光能转化效率Y（Ⅱ）、光合电子传递效率（ETR）及藻密度。结果发现:磷胁迫对藻密度、Fv/Fm、Y（Ⅱ）和ETR有显著影响。恢复实验中，Fv/Fm、Y（Ⅱ）、ETR等恢复速率与起始所受磷胁迫程度以及再添加的磷浓度呈正相关。与藻密度相比，Fv/Fm、Y（Ⅱ）、ETR等荧光参数可以在12 h内产生更显著的变化。本实验表明，在严重的磷胁迫条件下，磷的输入可以造成藻类的补偿生长；在小时尺度上叶绿素荧光参数随磷胁迫程度可以迅速做出响应。
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| 关 键 词： | 磷胁迫 叶绿素荧光参数 小时间尺度 |
| 收稿时间： | 2015-12-30 |
Response of Phaeodactylum tricornutum to different phosphorus stress and recovery in batch culture |
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| Wen-jiao LI,Jian-feng FENG,Lin ZHU,Yin-huan QIAO. Response of Phaeodactylum tricornutum to different phosphorus stress and recovery in batch culture[J]. Marine Environmental Science, 2016, 35(6): 894-900. DOI: 10.13634/j.cnki.mes.2016.06.015 |
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| Authors: | Wen-jiao LI Jian-feng FENG Lin ZHU Yin-huan QIAO |
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| Affiliation: | Key Laboratory of Pollution Processes and Environmental Criteria, Ministry of Education, Tianjin Key Laboratory of Environmental Remediation and Pollution Control, College of Environmental Science and Engineering, Nankai University, Tianjin 300071, China |
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| Abstract: | Five concentration of PO4-P (0, 0.566 μmol/L, 2.26 μmol/L, 9.05 μmol/L, 36.2 μmol/L) were set up to study the response to different phosphorus stress and recovery in batch culture.Fv/Fm, Y (Ⅱ), ETR and biomass was measured.Phosphorus was not added until the ninth day during ten-day experiments.Results showed that the concentration of phosphorus has a significant influence on biomass, Fv/Fm, Y (Ⅱ) and ETR.The recovery rates of Fv/Fm, Y (Ⅱ) and ETR were positively correlated with the initial and the added phosphorus concentrations.Overall, the input of phosphorus can cause compensatory growth in the severe phosphorus stress, and the chlorophyll fluorescence parameters can respond quickly within 12 h. |
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