| 海河口沉积物nirS型反硝化微生物多样性研究 |
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| 引用本文： | 王春艳,关道明,关翔宇. 海河口沉积物nirS型反硝化微生物多样性研究[J]. 海洋环境科学, 2020, 39(3): 374-378. DOI: 10.12111/j.mes20200308 |
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| 作者姓名： | 王春艳 关道明 关翔宇 |
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| 作者单位： | 1.南阳理工学院 生物与化学工程学院, 河南 南阳 473004 |
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| 基金项目： | 国家自然科学基金重点项目（NSFC 41731282） |
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| 摘 要： | 以海河口典型淡水、淡海水以及海水环境的沉积物样品为研究对象，采用克隆文库及典范对应分析等方法研究了nirS型反硝化微生物群落结构的多样性。3个站位共获得154条有效序列，97%序列相似性水平划分为66个OTUs，海水环境的沉积物站位nirS型反硝化微生物多样性最高。系统进化分析显示，全部OTUs序列被划分为7个簇，最相近序列的微生物主要来源于河口、富营养化海湾、人工湿地及海水养殖沉积物等环境。海河口具有独特的反硝化微生物群落分布格局，典范对应分析结果表明该区域的盐度、有机碳以及氮相关营养盐水平是影响其群落特征的重要因素。
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| 关 键 词： | 海河口 沉积物 nirS基因 反硝化微生物 多样性 |
| 收稿时间： | 2018-09-10 |
Diversity of nirS-type denitrifying bacteria in the sediments from Hai river estuary |
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| Chun-yan WANG,Dao-ming GUAN,Xiang-yu GUAN. Diversity of nirS-type denitrifying bacteria in the sediments from Hai river estuary[J]. Marine Environmental Science, 2020, 39(3): 374-378. DOI: 10.12111/j.mes20200308 |
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| Authors: | Chun-yan WANG Dao-ming GUAN Xiang-yu GUAN |
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| Affiliation: | 1.School of Civil Engineering, Nanyang Institute of Technology, Nanyang 473004, China |
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| Abstract: | In order to study the diversity of denitrifying bacteria in Hai river estuary, three sediment samples were collected from the freshwater, mixed-water and seawater environments of Hai River estuary and analyzed using clone library and canonical correspondence analysis (CCA). A total of 154 valid nirS sequences were grouped into 66 OTUs based on 97% sequence similarity. The diversity indices of those libraries showed the highest diversity of denitrifying community in the seawater sample. The phylogenetic analysis showed that the nirS sequences were divided into seven Clusters, which were highly similar to those of denitrifying bacteria from sediments of estuary, eutrophied bay, wetland and aquaculture wastewater. CCA indicated that salinity, organic carbon and nitrogen nutrient were the major contributing parameters for the diversity and spatial distribution of denitrifying bacteria of Hai river estuary. |
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