Spatial and seasonal variations in bacterial communities of the Yellow Sea by T-RFLP analysis |
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| Authors: | Hongyuan Wang Xiaolu Jiang Ya He Huashi Guan |
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| Affiliation: | 1. School of Medicine and Pharmacy, Ocean University of China, Qingdao 266003, China; 2. School of Medicine and Pharmacy, Ocean University of China, Qingdao 266003, China; 3. School of Environment, Beijing Normal University, Beijing 100875, China |
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| Abstract: | Four typical coastal sites (rocky shore, sandy shore, mud flat shore, and artificial harbor) at the Yellow Sea were chosen to investigate the spatial and seasonal variations in bacterial communities. This was accomplished by using terminal restriction fragment length polymorphism (T-RFLP) analysis of PCR amplified 16S rDNA fragments. Two kinds of tetrameric restriction enzymes, HhaI and MspI, were used in the experiment to depict the bacterial community diversity in different marine environments. It was found that the community compositions digested by the two enzymes separately were different. However, the results of bacterial community diversity derived from them were similar. The MDA analysis results of T-RFLP profiles coming from HhaI and MspI both exhibited a significant seasonal community shift for bacteria and a relatively low spatial variation among the four locations. With HhaI as the sample, the pair wise T-tests also revealed that variations were minor between each pair of marine environments, with R ranging from 0.198 to 0.349. However, the bacterial community structure in the mud flat site depicted a larger difference than each of the other three sites (R ranging from 0.282 to 0.349). |
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| Keywords: | terminal restriction fragment length polymorphism (T-RFLP) bacterial community structure marine microbial diversity HhaI MspI |
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