| 基于间接竞争抑制ELISA的2,2',4,4'-四溴联苯醚检测方法 |
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| 引用本文： | 刘婧婷, 谯华, 张彦峰, 孙红文, 刘洪涛. 基于间接竞争抑制ELISA的2,2',4,4'-四溴联苯醚检测方法[J]. 环境工程学报, 2017, 11(7): 4429-4436. doi: 10.12030/j.cjee.201605142 |
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| 作者姓名： | 刘婧婷 谯华 张彦峰 孙红文 刘洪涛 |
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| 作者单位： | 1.后勤工程学院国防建筑规划与环境工程系, 重庆 401311; 2.南开大学环境科学与工程学院, 天津 300071; 3.后勤工程学院军事油料应用与管理工程系, 重庆 401311 |
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| 基金项目： | 国家高技术研究发展计划（863计划）项目（2014AA7013037） 天津科技兴海项目（KJXH2012-22） 解放军后勤工程学院院青年科研基金资助项目（YQ15-410307） |
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| 摘 要： | 针对现有的检测多溴联苯醚（PBDEs）方法中存在的样品处理繁复、样品检测耗时以及费用昂贵等不足，开展了针对此类物质中代表性化合物2，2'，4，4'-四溴联苯醚（BDE-47）的间接竞争抑制酶联免疫吸附法（ELISA）的检测分析研究，并与气相色谱-质谱检测法、酶联免疫吸附分析试剂盒检测法进行对比分析。研究表明：间接竞争抑制ELISA法测定BDE-47的IC50值为0.11 mg·L-1，定量限为0.04 mg·L-1，检出范围为0.01~0.50 mg·L-1。该方法在满足复杂基质中微量目标物检测灵敏度及重现性要求下，具有检测更快捷、更经济实用的特点，宜适用于大批量此类环境样品的初期筛查。
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| 关 键 词： | 多溴联苯醚 酶联免疫吸附分析 间接竞争抑制 气相色谱-质谱 |
| 收稿时间： | 2016-08-14 |
Resarch of 2, 2', 4, 4'-Tetrabromodiphenyl Ether detection method based on indirect competitive inhibition enzyme-linked immunosorbent assay |
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| LIU Jingting, QIAO Hua, ZHANG Yanfeng, SUN Hongwen, LIU Hongtao. Resarch of 2, 2', 4, 4'-Tetrabromodiphenyl Ether detection method based on indirect competitive inhibition enzyme-linked immunosorbent assay[J]. Chinese Journal of Environmental Engineering, 2017, 11(7): 4429-4436. doi: 10.12030/j.cjee.201605142 |
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| Authors: | LIU Jingting QIAO Hua ZHANG Yanfeng SUN Hongwen LIU Hongtao |
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| Affiliation: | 1.Department of National Defense Building Architecture Planning & Environmental Engineering, Logistic Engineering University of People's Liberation Army, Chongqing 401311, China; 2.College of Environmental Science and Engineering, Nankai University, Tianjin 300071, China; 3.Department of Military oil Application and Management Engineering, Logistic Engineering University of People's Liberation Army, Chongqing 401311, China |
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| Abstract: | The existing methods for the detection of polybrominated diphenyl ethers (PBDEs) in complex samples face challenges like high costs and long sample testing times. This study assessed the indirect competitive inhibition enzyme-linked immunosorbent assay (ELISA) of 2, 2', 4, 4'-tetrabromodiphenyl ether (BDE-47) as a representative PBDE, comparing performance with those of gas chromatography-mass spectrometry (GC-MS) detection methods and ELISA kits. For the indirect competitive inhibition ELISA method, the value of IC50 was 0.11 mg·L-1, the limit of quantification was 0.04 mg·L-1, and the detection range was 0.01-0.50 mg·L-1. Meeting the requirements of sensitivity and reproducibility of detecting trace targets in a complicated matrix, ELISA is a more efficient, economic, and practical method suitable for early screening of large quantities of environmental samples. |
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| Keywords: | polybrominated diphenyl ethers enzyme-linked immunosorbent assay indirect competitive inhibition gas chromatography-mass |
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