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| 耐盐复合菌剂强化生物工艺处理高盐废水的快速启动 | |
| 引用本文： | 姜超, 隋倩雯, 陈梅雪, 柴玉峰, 张岚, 柳蒙蒙, 张兆昌, 杨金, 魏源送. 耐盐复合菌剂强化生物工艺处理高盐废水的快速启动[J]. 环境工程学报, 2017, 11(7): 3929-3935. doi: 10.12030/j.cjee.201604122 |
| 作者姓名： | 姜超 隋倩雯 陈梅雪 柴玉峰 张岚 柳蒙蒙 张兆昌 杨金 魏源送 |
| 作者单位： | 1. 中国科学院生态环境研究中心, 北京 100085; 2. 中国科学院大学, 北京 100049; 3. 中国矿业大学(北京)化学与环境工程学院, 北京 100083; 4. 北京维奥思环境工程有限公司, 北京 100097 |
| 基金项目： | 国家自然科学基金资助项目（21577161，41501513） |
| 摘 要： | 从城市污水处理厂的活性污泥中驯化分离出2株耐盐高效菌：地衣芽孢杆菌（Bacillus licheniformis ）O1和枯草芽孢杆菌（Bacillus subtilis）Y5制备复合菌剂，用于高盐生活污水生物处理工艺快速启动研究。研究表明，在SBR系统中连续投加复合菌剂（制备的配比为1∶1），在30 d完成快速启动（TOC去除率>85%），并且在整个启动过程中，TOC的去除率都能够稳定保持在80%左右，而负载复合菌剂填料的投入可获得更稳定的出水水质。通过高通量测序与OTU分类，高盐废水的配入使得活性污泥微生物群落结构发生显著改变，并且在工艺启动后，所投加的耐盐高效菌O1和Y5在活性污泥微生物总量中所占比例由1.31%升高至6.13%，说明O1和Y5能够在小试SBR中长期存留，并逐渐成为优势种属之一。 |
| 关 键 词： | 高盐废水 耐盐复合菌剂 快速启动 高通量测序 微生物群落结构 |
| 收稿时间： | 2016-05-30 |
Quick start of high saline wastewater biological treatment technology strengthened by composite salt-tolerant microbe |
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| JIANG Chao, SUI Qianwen, CHEN Meixue, CHAI Yufeng, ZHANG Lan, LIU Mengmeng, ZHANG Zhaochang, YANG Jin, WEI Yuansong. Quick start of high saline wastewater biological treatment technology strengthened by composite salt-tolerant microbe[J]. Chinese Journal of Environmental Engineering, 2017, 11(7): 3929-3935. doi: 10.12030/j.cjee.201604122 | |
| Authors: | JIANG Chao SUI Qianwen CHEN Meixue CHAI Yufeng ZHANG Lan LIU Mengmeng ZHANG Zhaochang YANG Jin WEI Yuansong |
| Affiliation: | 1. Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China; 2. University of Chinese Academy of Sciences, Beijing 100049, China; 3. China University of Mining & Technology(Beijing) School of Chemical & Environmental Engineering, Beijing 100083, China; 4. Beijing Wealth Environmental Engineering Co. Ltd., Beijing 100097, China |
| Abstract: | The composite salt-tolerant microbe was prepared by the two salt-tolerant bacteria species:Bacillus licheniformis O1 and Bacillus subtilis Y5, which were isolated from the active sludge of a sewage treatment plant.The complex salt-tolerant microorganisms were inoculated into a high saline wastewater biological treatment system in order to quick start.The results demonstrated that a 1:1 ratio was best suited to the preparation of the composite salt-tolerant microbe, through repeatedly dosing 10%(wt%)of composite salt-tolerant microbe, which could help lab-scale sequencing batch reactor (SBR) complete the start-up (TOC removal rate >85%) within 30 days.During the whole start-up period, the removal ratio of TOC was kept at 80%, and the inputting of carriers loaded with the composite salt-tolerant microbe stabilized the treatment efficiency.High-throughput sequencing and an operational taxonomic unit classification showed that the composite salt-tolerant microbe inoculating was responsible for the proportion of O1 and Y5 of the total activated sludge microorganisms increasing from 1.31 to 6.13% after the process began, indicating that O1 and Y5 had achieved a long-term retention in the lab-scale SBR, and had gradually become the dominant genera. |
| Keywords: | high saline wastewater composite salt-tolerant microbe quick start High-throughput sequencing microbiology community |
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