| CO2倍增和施氮对水稻不同生长期土壤反硝化细菌丰度的影响 |
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| 引用本文： | 陈娜,刘毅,肖谋良,袁红朝,葛体达,徐华勤,唐启源. CO2倍增和施氮对水稻不同生长期土壤反硝化细菌丰度的影响[J]. 环境科学研究, 2019, 32(4): 683-691. DOI: 10.13198/j.issn.1001-6929.2019.01.05 |
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| 作者姓名： | 陈娜 刘毅 肖谋良 袁红朝 葛体达 徐华勤 唐启源 |
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| 作者单位： | 湖南农业大学农学院,湖南 长沙 410128;中国科学院亚热带农业生态研究所,亚热带农业生态过程重点实验室,湖南 长沙 410125;中国科学院亚热带农业生态研究所,亚热带农业生态过程重点实验室,湖南 长沙 410125;湖南农业大学农学院,湖南 长沙,410128 |
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| 基金项目： | 国家自然科学基金项目（No.41771300，41771334）；国家水稻产业技术体系（No.CARS-01-26） |
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| 摘 要： | 随着全球气候变化的不断加剧，大气CO2浓度呈明显增加趋势，这将间接影响土壤-植物-微生物系统的氮循环过程.为研究典型水稻土壤反硝化细菌对CO2浓度升高的响应规律和机制，借助水稻密闭培养箱，运用实时荧光定量聚合酶链式反应（Real-Time qPCR）分子技术，设置不施氮（0 mg/kg）和常规施氮（100 mg/kg）2个处理，研究CO2倍增对水稻不同生长期土壤关键反硝化功能细菌（narG、nirK和nirS型）丰度的影响.结果表明:①在2种施氮水平，CO2倍增显著促进了水稻分蘖期、孕穗期、扬花期和成熟期水稻根系生长（增幅为2.96%~28.4%）、地上部生物量增加（增幅为7.1%~107.3%）以及成熟期籽粒干质量的增加（增幅为19.5%和38.0%），具有显著的增产效应.②反硝化细菌丰度对CO2倍增的响应与生育期及施氮水平有关，CO2倍增在2个施氮水平均抑制分蘖期反硝化细菌的繁殖，显著增加孕穗期反硝化细菌数量；在水稻扬花期，CO2倍增促进了施氮处理narG和nirS型反硝化细菌数量的增加，在成熟期抑制未施氮处理下narG、nirK和nirS型反硝化细菌的生长.另外，narG、nirK、nirS型反硝化细菌丰度整体表现为narG > nirS > nirK，且随水稻的生长，其在成熟期的丰度均呈降低趋势.nirK和nirS基因同属亚硝酸还原酶，但nirS基因丰度高于nirK，且对CO2倍增和施氮的响应有所差异.研究显示，CO2倍增可显著增加水稻生长和产量，不同施氮水平对稻田土壤反硝化细菌丰度的影响存在差异.
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| 关 键 词： | CO2 倍增 水稻土 水稻生长 反硝化细菌 丰度 |
| 收稿时间： | 2018-03-22 |
| 修稿时间： | 2018-12-19 |
Effect of Elevated CO2 and Nitrogen Application on the Abundance of Soil Denitrifying Bacteria in Different Growth Stages of Rice |
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| CHEN N,LIU Yi,XIAO Mouliang,YUAN Hongzhao,GE Tid,XU Huaqin and TANG Qiyuan. Effect of Elevated CO2 and Nitrogen Application on the Abundance of Soil Denitrifying Bacteria in Different Growth Stages of Rice[J]. Research of Environmental Sciences, 2019, 32(4): 683-691. DOI: 10.13198/j.issn.1001-6929.2019.01.05 |
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| Authors: | CHEN N LIU Yi XIAO Mouliang YUAN Hongzhao GE Tid XU Huaqin TANG Qiyuan |
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| Affiliation: | 1.College of Agronomy, Hunan Agriculture University, Changsha 410128, China2.Key Laboratory of Agro-Ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, China |
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| Abstract: | The atmospheric CO2 concentration has an obvious rising trend with the increasing global climate change, which will indirectly affect the nitrogen cycling process of the soil-plant-microbe system. In order to investigate the mechanisms of the rising CO2 effect on denitrifying bacteria in paddy soil, we used the airtight rice incubator and the real-time PCR technology to investigate the effect of the elevating CO2 concentration on the abundance of narG-, nirK-and nirS-containing bacterial communities at two nitrogen levels. The results showed that the elevating CO2 concentration significantly increased the biomass of roots and aboveground parts (2.96%-28.4%, 7.1%-107.3%) at the tillering, booting, blooming and maturing stages either without nitrogen or with regular nitrogen application. Meantime, the dry weight of grains at the two levels of nitrogen application increased (19.5%, 38.0%) at the maturing stage, indicating that the rice yield was stimulated by the elevating CO2 concentration;Response of denitrifying bacteria to elevating CO2 concentration was related to growth stage and nitrogen application level:the elevating CO2 decreased the number of denitrifying bacteria at tillering stage, but increased that at booting stage; The elevating CO2 obviously increased the abundance of narG-, nirS-containing communities in nitrogen application treatment at the booting stage, and decreased the abundance of narG-, nirK-and nirS-containing communities at the maturing stage. In addition, the abundance of narG-, nirK-and nirS-containing communities were narG > nirS > nirK, and they decreased with the increase of rice growth. Both nirK and nirS genes belong to nitrite reductase, but the abundance of nirS gene was higher than that of nirK, and their responses to elevating CO2 and nitrogen application were different. Overall, the results showed that the elevating CO2 significantly increased rice growth and yield, and the effects of CO2 on the abundance of denitrifying bacteria in paddy soil were different with different nitrogen application. |
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| Keywords: | elevated CO2 paddy soil rice growth denitrifying bacteria abundance |
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