| 土著PHA合成菌回注法提高活性污泥积累聚羟基脂肪酸酯能力 |
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| 引用本文： | 邓 飞,曾 猛,刘 娜,王 聪,孙村民,王淑芳,宋存江. 土著PHA合成菌回注法提高活性污泥积累聚羟基脂肪酸酯能力[J]. 环境科学研究, 2008, 21(4): 14-19 |
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| 作者姓名： | 邓 飞 曾 猛 刘 娜 王 聪 孙村民 王淑芳 宋存江 |
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| 作者单位： | 1.南开大学 生命科学学院 微生物学系,分子微生物学与技术教育部重点实验室,天津 300071 |
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| 基金项目： | 国家高技术研究发展计划(863)项目 , 天津市科技攻关专项 |
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| 摘 要： | 在活性污泥驯化之初加入少量土著聚羟基脂肪酸酯(PHA)合成菌(0.10～0.25 g/L)，通过10 d厌氧-好氧或好氧-沉淀方式进行污泥驯化后，以葡萄糖或乙酸钠为碳源进行PHA发酵.研究了驯化前后土著PHA合成菌回注组和对照组驯化过程中的总菌数，PHA合成菌数的变化，以及对PHA发酵产率的影响.结果表明:在回注组污泥中PHA合成菌的增长速率和增长量均高于对照组，各种污泥积累PHA占挥发性悬浮固体的比例提高了21.44%～43.18%.
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| 关 键 词： | 剩余活性污泥 土著PHA合成菌 厌氧-好氧驯化 好氧-沉淀驯化 聚羟基脂肪酸酯 |
| 收稿时间： | 2007-09-20 |
| 修稿时间： | 2008-01-17 |
Improvement of PHA Accumulation Ability for Excess Activated Sludge by Adding Native PHA-Synthesis-Bacteria |
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| DENG Fei,ZENG Meng,LIU N,WANG Cong,SUN Cun-min,WANG Shu-fang and SONG Cun-jiang. Improvement of PHA Accumulation Ability for Excess Activated Sludge by Adding Native PHA-Synthesis-Bacteria[J]. Research of Environmental Sciences, 2008, 21(4): 14-19 |
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| Authors: | DENG Fei ZENG Meng LIU N WANG Cong SUN Cun-min WANG Shu-fang SONG Cun-jiang |
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| Affiliation: | Department of Microbiology, Life Science College; Key Laboratory of MolecularMicrobiology and Technology of Education Ministry, Nankai University, Tianjin 300071, China;Department of Microbiology, Life Science College; Key Laboratory of MolecularMicrobiology and Technology of Education Ministry, Nankai University, Tianjin 300071, China;Department of Microbiology, Life Science College; Key Laboratory of MolecularMicrobiology and Technology of Education Ministry, Nankai University, Tianjin 300071, China;Department of Microbiology, Life Science College; Key Laboratory of MolecularMicrobiology and Technology of Education Ministry, Nankai University, Tianjin 300071, China;Department of Microbiology, Life Science College; Key Laboratory of MolecularMicrobiology and Technology of Education Ministry, Nankai University, Tianjin 300071, China;Life Science College, Key Laboratory of Bioactive Materials for Education Ministry, Nankai University, Tianjin 300071, China;Department of Microbiology, Life Science College; Key Laboratory of MolecularMicrobiology and Technology of Education Ministry, Nankai University, Tianjin 300071, China;Life Science College, Key Laboratory of Bioactive Materials for Education Ministry, Nankai University, Tianjin 300071, China |
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| Abstract: | Some native PHA-synthesis-bacteria (0.10～0.25 g/L) were added at initial stages of activated sludge domestication. The numbers of total microbe and PHA-synthesis-bacteria in activated sludge were estimated during domestication ofanaerobic-aerobic or aerobic- settling condition for 10 days. PHA fermentation was studied by glucose or sodium acetate trihydrate as carbon source. Results showed that the proliferation acceleration of PHA-synthesis-bacteria in activated sludge was increased after the native PHA-synthesis-bacteria were added at initialstages of domestication to excess activated sludge. Simultaneously, the yields of PHA, i.e. the percents of PHA/VSS, were higher than that of control runs. They were raised by 21.44% to 43.18%. |
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