| 共振能量转移同步荧光猝灭法测定水体中孔雀石绿 |
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| 引用本文： | 毛永强, 李娜. 共振能量转移同步荧光猝灭法测定水体中孔雀石绿[J]. 环境工程学报, 2015, 9(5): 2055-2060. doi: 10.12030/j.cjee.20150504 |
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| 作者姓名： | 毛永强 李娜 |
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| 作者单位： | 1. 辽宁工程技术大学理学院, 阜新 123000; 2. 辽宁工程技术大学安全科学与工程学院, 矿山热动力灾害与防治教育部重点实验室, 阜新 123000 |
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| 基金项目： | "十二五"国家科技支撑计划项目(2013BAK06B07) 辽宁省教育厅科学研究项目(L2013138) 辽宁工程技术大学博士启动基金(13-1019) 辽宁省大学生创新创业训练计划项目(201410147014,201410147046) |
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| 摘 要： | 采用L-半胱氨酸、巯基乙酸为稳定剂水相合成CdTe量子点,且以CdTe量子点为能量供体、孔雀石绿为能量受体建立荧光共振能量转移体系,从而实现孔雀石绿含量的同步荧光猝灭法测定。结果表明,当固定波长差为220 nm时,CdTe量子点的同步荧光最大发射波长位于323 nm。在最优实验条件下,体系的同步荧光淬灭强度与孔雀石绿的浓度在0.5~10.0 μmol/L范围内呈良好的线性关系,相关系数为0.9995,方法的检出限为10 nmol/L。该方法用于实际水样中孔雀石绿含量的测定,加标回收率为94%~105%。
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| 关 键 词： | CdTe量子点 荧光共振能量转移 孔雀石绿 同步荧光 |
| 收稿时间： | 2015-01-23 |
Fluorescence resonance energy transfer quenching method for determination of malachite green in water using synchronous fluorescence spectrometry |
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| Mao Yongqiang, Li Na. Fluorescence resonance energy transfer quenching method for determination of malachite green in water using synchronous fluorescence spectrometry[J]. Chinese Journal of Environmental Engineering, 2015, 9(5): 2055-2060. doi: 10.12030/j.cjee.20150504 |
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| Authors: | Mao Yongqiang Li Na |
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| Affiliation: | 1. College of Science, Liaoning Technical University, Fuxin 123000, China; 2. Key Laboratory of Mine Thermodynamic Disasters and Control of Ministry of Education, College of Safety Science and Engineering, Liaoning Technical University, Fuxin 123000, China |
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| Abstract: | Water-soluble CdTe quantum dots (QDs) were synthesized using thioglycolic acid and L-cysteine as stabilizer. The fluorescence resonance energy transfer (FRET) system was constructed, where CdTe QDs and malachite green (MG) served as energy donors and energy acceptor respectively. Based on the FRET system, a new method of synchronous fluorescence spectrometry to determine MG was established. When Δλ=220 nm, the maximum emission wavelength of synchronous fluorescence was 323 nm. Under the optimal conditions, a good linearity was obtained between the synchronous fluorescence intensity of CdTe QDs and concentration of MG from 0.5 to 10.0 μmol/L. The correlation coefficient was 0.9995 and the limit of detection was 10 nmol/L. The proposed method was applied for the determination of MG in real water samples with spiked recoveries of 94%~105%. |
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| Keywords: | CdTe quantum dots malachite green fluorescence resonance energy transfer synchronous fluorescence spectrometry |
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