Remarkable Amphibian Biomass and Abundance in an Isolated Wetland: Implications for Wetland Conservation

Abstract:  Despite the continuing loss of wetland habitats and associated declines in amphibian populations, attempts to translate wetland losses into measurable losses to ecosystems have been lacking. We estimated the potential productivity from the amphibian community that would be compromised by the loss of a single isolated wetland that has been protected from most industrial, agricultural, and urban impacts for the past 54 years. We used a continuous drift fence at Ellenton Bay, a 10-ha freshwater wetland on the Savannah River Site, near Aiken, South Carolina (U.S.A.), to sample all amphibians for 1 year following a prolonged drought. Despite intensive agricultural use of the land surrounding Ellenton Bay prior to 1951, we documented 24 species and remarkably high numbers and biomass of juvenile amphibians (>360,000 individuals; >1,400 kg) produced during one breeding season. Anurans (17 species) were more abundant than salamanders (7 species), comprising 96.4% of individual captures. Most (95.9%) of the amphibian biomass came from 232095 individuals of a single species of anuran (southern leopard frog [Rana sphenocephala ]). Our results revealed the resilience of an amphibian community to natural stressors and historical habitat alteration and the potential magnitude of biomass and energy transfer from isolated wetlands to surrounding terrestrial habitat. We attributed the postdrought success of amphibians to a combination of adult longevity (often >5 years), a reduction in predator abundance, and an abundance of larval food resources. Likewise, the increase of forest cover around Ellenton Bay from <20% in 1951 to >60% in 2001 probably contributed to the long-term persistence of amphibians at this site. Our findings provide an optimistic counterpoint to the issue of the global decline of biological diversity by demonstrating that conservation efforts can mitigate historical habitat degradation.     

A Resistant-Kernel Model of Connectivity for Amphibians that Breed in Vernal Pools

Abstract:  Pool-breeding amphibian populations operate at multiple scales, from the individual pool to surrounding upland habitat to clusters of pools. When metapopulation dynamics play a role in long-term viability, conservation efforts limited to the protection of individual pools or even pools with associated upland habitat may be ineffective over the long term if connectivity among pools is not maintained. Connectivity becomes especially important and difficult to assess in regions where suburban sprawl is rapidly increasing land development, road density, and traffic rates. We developed a model of connectivity among vernal pools for the four ambystomatid salamanders that occur in Massachusetts and applied it to the nearly 30,000 potential ephemeral wetlands across the state. The model was based on a modification of the kernel estimator (a density estimator commonly used in home range studies) that takes landscape resistance into account. The model was parameterized with empirical migration distances for spotted salamanders ( Ambystoma maculatum ), dispersal distances for marbled salamanders ( A. opacum ), and expert-derived estimates of landscape resistance. The model ranked vernal pools in Massachusetts by local, neighborhood, and regional connectivity and by an integrated measure of connectivity, both statewide and within ecoregions. The most functionally connected pool complexes occurred in southeastern and northeastern Massachusetts, areas with rapidly increasing suburban development. In a sensitivity analysis estimates of pool connectivity were relatively insensitive to uncertainty in parameter estimates, especially at the local and neighborhood scales. Our connectivity model could be used to prioritize conservation efforts for vernal-pool amphibian populations at broader scales than traditional pool-based approaches.     

Amphibian occurrence is influenced by current and historic landscape characteristics.

Human-induced habitat loss and degradation are major threats to wetland species as reflected in the fact that wetlands have declined by more than 50% in Europe and North America during the last century. Both current and historic land-use patterns are likely to be significant determinants of wetland species' distributions; however their relative importance is often unknown. We studied the importance of local (study pond) and landscape (current and 18th-century landscape) characteristics in explaining the occurrence and species richness of amphibians (Rana arvalis, Bufo bufo, and Triturus vulgaris) on the Swedish island of Gotland, where more than 40% of wetlands have been lost since the 18th century. Current local habitat characteristics were the strongest determinants of occurrence for all study species. Additionally, species occurrence was related to current and historic landscape characteristics, which generally explained equal amounts of the variation in species-occurrence data. The proportions of both current and historic arable land were negative determinants of amphibian occurrence and species richness, indicating that agricultural land use may have an overall negative impact on amphibians, and that amphibians may occur less frequently in areas with a long agricultural history. Likewise, historic forest area was positively related to B. bufo occurrence and species richness, whereas current forests had no significant effects, suggesting that there may be a lag in the response of amphibians to agriculture-mediated habitat loss. Our results suggest that historic land-use patterns may influence current amphibian populations and that inclusion of historic land-use information could be a valuable tool in future studies on amphibian-habitat relations.     

Roles of Patch Characteristics,Drought Frequency,and Restoration in Long‐Term Trends of a Widespread Amphibian

Despite the high profile of amphibian declines and the increasing threat of drought and fragmentation to aquatic ecosystems, few studies have examined long‐term rates of change for a single species across a large geographic area. We analyzed growth in annual egg‐mass counts of the Columbia spotted frog (Rana luteiventris) across the northwestern United States, an area encompassing 3 genetic clades. On the basis of data collected by multiple partners from 98 water bodies between 1991 and 2011, we used state‐space and linear‐regression models to measure effects of patch characteristics, frequency of summer drought, and wetland restoration on population growth. Abundance increased in the 2 clades with greatest decline history, but declined where populations are considered most secure. Population growth was negatively associated with temporary hydroperiods and landscape modification (measured by the human footprint index), but was similar in modified and natural water bodies. The effect of drought was mediated by the size of the water body: populations in large water bodies maintained positive growth despite drought, whereas drought magnified declines in small water bodies. Rapid growth in restored wetlands in areas of historical population declines provided strong evidence of successful management. Our results highlight the importance of maintaining large areas of habitat and underscore the greater vulnerability of small areas of habitat to environmental stochasticity. Similar long‐term growth rates in modified and natural water bodies and rapid, positive responses to restoration suggest pond construction and other forms of management can effectively increase population growth. These tools are likely to become increasingly important to mitigate effects of increased drought expected from global climate change. Papeles de las Características del Fragmento, Frecuencia de Sequía y Restauración en las Tendencias a Largo Plazo de un Anfibio Ampliamente Distribuido     

Amphibian Breeding Distribution in an Urbanized Landscape

Abstract:  Amphibians commonly use wetlands for breeding habitat, and given the concern about their ongoing global declines, the effects of urbanization on the breeding distribution of amphibians need to be quantified. Thus, we conducted a survey of the larval amphibian community in central Pennsylvania (U.S.A.) wetlands along an urbanization gradient. Wetlands in urban areas had less surrounding forest and wetlands and greater road density than rural wetlands. Urbanization was also associated with increases in hydroperiod (i.e., wetland permanency) and the presence of fish predators. Moreover, urban wetlands had lower larval amphibian species richness than rural wetlands. This decrease in richness was attributable to a decrease in occurrence of wood frogs ( Rana sylvatica ) and ambystomatid salamanders ( Ambystoma maculatum and A. jeffersonianum ) in urban sites. Wood frogs and ambystomatid salamanders were positively associated with the amount of forest surrounding sites and negatively associated with hydroperiod. As a result, we hypothesize that these species are sensitive to the effects of urban development. The remaining species in this study appear fairly resilient to the effects of urbanization. These data demonstrate the importance of quantifying both local and landscape attributes when describing the factors that limit the breeding distribution of amphibians. We recommend that to preserve amphibian biodiversity in urbanized landscapes, it is best to focus on regional diversity, which protects a variety of sites that encompass various hydroperiods, have adequate buffer habitat, and are connected by dispersal routes.     

Rapid Increases and Time‐Lagged Declines in Amphibian Occupancy after Wildfire

Climate change is expected to increase the frequency and severity of drought and wildfire. Aquatic and moisture‐sensitive species, such as amphibians, may be particularly vulnerable to these modified disturbance regimes because large wildfires often occur during extended droughts and thus may compound environmental threats. However, understanding of the effects of wildfires on amphibians in forests with long fire‐return intervals is limited. Numerous stand‐replacing wildfires have occurred since 1988 in Glacier National Park (Montana, U.S.A.), where we have conducted long‐term monitoring of amphibians. We measured responses of 3 amphibian species to fires of different sizes, severity, and age in a small geographic area with uniform management. We used data from wetlands associated with 6 wildfires that burned between 1988 and 2003 to evaluate whether burn extent and severity and interactions between wildfire and wetland isolation affected the distribution of breeding populations. We measured responses with models that accounted for imperfect detection to estimate occupancy during prefire (0–4 years) and different postfire recovery periods. For the long‐toed salamander (Ambystoma macrodactylum) and Columbia spotted frog (Rana luteiventris), occupancy was not affected for 6 years after wildfire. But 7–21 years after wildfire, occupancy for both species decreased ≥25% in areas where >50% of the forest within 500 m of wetlands burned. In contrast, occupancy of the boreal toad (Anaxyrus boreas) tripled in the 3 years after low‐elevation forests burned. This increase in occupancy was followed by a gradual decline. Our results show that accounting for magnitude of change and time lags is critical to understanding population dynamics of amphibians after large disturbances. Our results also inform understanding of the potential threat of increases in wildfire frequency or severity to amphibians in the region. Incrementos Rápidos y Declinaciones Desfasadas en la Ocupación de Anfibios Después de un Incendio     

Evaluating the Effects of Anthropogenic Stressors on Source‐Sink Dynamics in Pond‐Breeding Amphibians

Although interwetland dispersal is thought to play an important role in regional persistence of pond‐breeding amphibians, few researchers have modeled amphibian metapopulation or source‐sink dynamics. Results of recent modeling studies suggest anthropogenic stressors, such as pollution, can negatively affect density and population viability of amphibians breeding in isolated wetlands. Presumably population declines also result in reduced dispersal to surrounding (often uncontaminated) habitats, potentially affecting dynamics of nearby populations. We used our data on the effects of mercury (Hg) on the American toad ( Bufo americanus) as a case study in modeling the effects of anthropogenic stressors on landscape‐scale amphibian dynamics. We created a structured metapopulation model to investigate regional dynamics of American toads and to evaluate the degree to which detrimental effects of Hg contamination on individual populations can disrupt interpopulation dynamics. Dispersal from typical American toad populations supported nearby populations that would otherwise have been extirpated over long time scales. Through support of such sink populations, dispersal between wetland‐associated subpopulations substantially increased overall productivity of wetland networks, but this effect declined with increasing interwetland distance and decreasing wetland size. Contamination with Hg substantially reduced productivity of wetland‐associated subpopulations and impaired the ability of populations to support nearby sinks within relevant spatial scales. Our results add to the understanding of regional dynamics of pond‐breeding amphibians, the wide‐reaching negative effects of environmental contaminants, and the potential for restoration or remediation of degraded habitats. Evaluación de los Efectos de Estresantes Antropogénicos sobre la Dinámica Fuente‐Vertedero en Anfibios que se Reproducen en Charcas     

Impacts of road deicing salt on the demography of vernal pool-breeding amphibians.

Deicing agents, primarily road salt, are applied to roads in 26 states in the United States and in a number of European countries, yet the scale of impacts of road salt on aquatic organisms remains largely under-studied. The issue is germane to amphibian conservation because both adult and larval amphibians are known to be particularly sensitive to changes in their osmolar environments. In this study, we combined survey, experimental, and demographic modeling approaches to evaluate the possible effects of road salt on two common vernal-pond-breeding amphibian species, the spotted salamander (Ambystoma maculatum) and the wood frog (Rana sylvatica). We found that in the Adirondack Mountain Region of New York (USA), road salt traveled up to 172 m from the highway into wetlands. Surveys showed that egg mass densities of spotted salamanders (A. maculatum) and wood frogs (R. sylvatica) were two times higher in forest pools than roadside pools, but this pattern was better explained by road proximity than by increased salinity. Experiments demonstrated that embryonic and larval survival were reduced at moderate (500 muS) and high conductivities (3000 muS) in A. maculatum and at high conductivities in R. sylvatica. Demographic models suggest that such egg and larval stage effects of salt may have important impacts on populations near roads, particularly in the case of A. maculatum, for which salt exposure may lead to local extinction. For both species, the effect of road salt was dependent upon the strength of larval density dependence and declined rapidly with distance from the roadside, with the greatest negative effects being limited to within 50 m. Based on this evidence, we argue that efforts to protect local populations of A. maculatum and R. sylvatica in roadside wetlands should, in part, be aimed at reducing application of road salt near wetlands with high conductivity levels.     

Testing wetland features to increase amphibian reproductive success and species richness for mitigation and restoration

Aquatic habitat features can directly influence the abundance, species richness, and quality of juvenile amphibians recruited into adult populations. We examined the influences of within-wetland slope, vegetation, and stocked mosquito fish (Gambusia affinis) on amphibian metamorph production and species richness during the first two years post-construction at 18 experimental wetlands in northeast Missouri (U.S.A.) grasslands. We used an information theoretic approach (AICc) to rank regression models representing total amphibian metamorph production, individual amphibian species metamorph production, and larval amphibian species richness. Total amphibian metamorph production was greatest at shallow-sloped, fish-free wetlands during the first year, but shallow-sloped wetlands with high vegetation cover were best the second year. Species richness was negatively associated with fish and positively associated with vegetation in both survey years. Leopard frog (Rana blairi/sphenocephala complex) metamorph quality, based on average metamorph size, was influenced by slope and the number of cohorts in the wetland. However, the tested variables had little influence on the size of American toads (Bufo americanus) or boreal chorus frogs (Pseudacris maculata). Our results indicate that wetlands designed to act as functional reproductive habitat for amphibians should incorporate shallows, high amounts of planted or naturally established vegetation cover, and should be fish-free.     

Terrestrial Buffer Zones and Wetland Conservation: A Case Study of Freshwater Turtles in a Carolina Bay

Because freshwater wetlands often support diverse and unique species assemblages, wetland loss is a primary concern in biological conservation. U. S. federal statutes protect many wetlands by deterring development within delineated borders that segregate wetland habitats from upland regions. In addition, some state and local jurisdictions mandate buffer zones that afford varying levels of protection to upland habitats adjacent to wetlands. We used geographic information system analysis to test the adequacy of federal and state wetland protection statutes by determining the degree to which protected acreage encompassed the habitats freshwater turtles needed to complete their life cycles. Two critical life-cycle stages, nesting and terrestrial hibernation, occurred exclusively beyond wetland boundaries delineated under federal guidelines. The most stringent state buffer zone insulated 44% of nest and hibernation sites. Our data indicate that the freshwater turtles examined in this study required a 275-m upland buffer zone to protect 100% of the nest and hibernation sites. Insulating 90% of the sites required a 73-m buffer zone. We suggest that the habitat needs of freshwater turtles demonstrate the dependence of wetland biodiversity on the preservation of adequate amounts of upland habitats adjacent to wetlands.     

Effects of Agricultural Cultivation on Demographics of Southern High Plains Amphibians

Abstract:  Anthropogenic disturbance of landscapes surrounding wetlands is considered a factor in local and global amphibian declines. Few data exist on the effects of agricultural cultivation of wetland watersheds on amphibians, and results from previous studies are contradictory. Our objective was to test the effects of general anthropogenic land use (cultivation vs. grassland) on the demographics of seven species and three age classes of amphibians in the Southern High Plains of Texas. We partially enclosed 16 playa wetlands (4 per land use per year) with drift fences and pitfall traps and monitored relative daily abundance and diversity from 16 May to 17 October 1999 and 19 April to 18 August 2000. In general, relative abundance (i.e., average daily capture) of New Mexico and plains spadefoots (  Spea multiplicata, S. bombifrons ) was greater at cropland than grassland playas; the abundance of other species and diversity of the amphibian assemblage was not affected by land use. Also, abundance generally was greater in 1999 than 2000 for metamorph spadefoots and barred tiger salamanders ( Ambystoma tigrinum mavortium ). Differences in spadefoot abundance between land-use types may have been related to low species-specific vagility, resulting in increased nestedness within disturbed landscapes and reduced abundance of a potential keystone intraguild predator in cropland playas. The yearly difference in amphibian abundance was likely related to annual precipitation, which influenced wetland hydroperiod. Agricultural cultivation surrounding wetlands is associated with the increased abundance of some amphibian species, but other demographic and fitness parameters—such as temporal demographics, body size, and diet diversity—may be negatively affected.     

Suitability of Golf Course Ponds for Amphibian Metamorphosis When Bullfrogs Are Removed

Abstract:  Managing areas designed for human recreation so that they are compatible with natural amphibian populations can reduce the negative impacts of habitat destruction. We examined the potential for amphibians to complete larval development in golf course ponds in the presence or absence of overwintered bullfrog tadpoles ( Rana catesbeiana ), which are frequently found in permanent, human-made ponds. We reared larval American toads ( Bufo americanus ), southern leopard frogs ( R. sphenocephala ), and spotted salamanders ( Ambystoma maculatum ) with 0 or 5 overwintered bullfrog tadpoles in field enclosures located in ponds on golf courses or in experimental wetlands at a reference site. Survival to metamorphosis of American toads, southern leopard frogs, and spotted salamanders was greater in ponds on golf courses than at reference sites. We attributed this increased survival to low abundance of insect predators in golf course ponds. The presence of overwintered bullfrogs, however, reduced the survival of American toads, southern leopard frogs, and spotted salamanders reared in golf course ponds, indicating that the suitability of the aquatic habitats for these species partly depended on the biotic community present. Our results suggest that ponds in human recreational areas should be managed by maintaining intermediate hydroperiods, which will reduce the presence of bullfrog tadpoles and predators, such as fish, and which may allow native amphibian assemblages to flourish.     

Effects of Landscape Composition and Wetland Fragmentation on Frog and Toad Abundance and Species Richness in Iowa and Wisconsin, U.S.A.

Abstract: Management of amphibian populations to reverse recent declines will require defining high-quality habitat for individual species or groups of species, followed by efforts to retain or restore these habitats on the landscape. We examined landscape-level habitat relationships for frogs and toads by measuring associations between relative abundance and species richness based on survey data derived from anuran calls and features of land-cover maps for Iowa and Wisconsin. The most consistent result across all anuran guilds was a negative association with the presence of urban land. Upland and wetland forests and emergent wetlands tended to be positively associated with anurans. Landscape metrics that represent edges and patch diversity also had generally positive associations, indicating that anurans benefit from a complex of habitats that include wetlands. In Iowa the most significant associations with relative abundance were the length of the edge between wetland and forest ( positive) and the presence of urban land (negative). In Wisconsin the two most significant associations with relative abundance were forest area and agricultural area ( both positive). Anurans had positive associations with agriculture in Wisconsin but not in Iowa. Remnant forest patches in agricultural landscapes may be providing refuges for some anuran species. Differences in anuran associations with deep water and permanent wetlands between the two states suggest opportunities for management action. Large-scale maps can contribute to predictive models of amphibian habitat use, but water quality and vegetation information collected from individual wetlands will likely be needed to strengthen those predictions. Landscape habitat analyses provide a framework for future experimental and intensive research on specific factors affecting the health of anurans.     

Lethal Effects of Water Quality on Threatened California Salamanders but Not on Co‐Occurring Hybrid Salamanders

Biological invasions and habitat alteration are often detrimental to native species, but their interactions are difficult to predict. Interbreeding between native and introduced species generates novel genotypes and phenotypes, and human land use alters habitat structure and chemistry. Both invasions and habitat alteration create new biological challenges and opportunities. In the intensively farmed Salinas Valley, California (U.S.A.), threatened California tiger salamanders (Ambystoma californiense) have been replaced by hybrids between California tiger salamander and introduced barred tiger salamanders (Ambystoma tigrinum mavortium). We conducted an enclosure experiment to examine the effects habitat modification and relative frequency of hybrid and native California tiger salamanders have on recruitment of salamanders and their prey, Pacific chorus frogs (Pseudacris regilla). We tested whether recruitment differed among genetic classes of tiger salamanders (hybrid or native) and pond hydroperiod (seasonal or perennial). Roughly 6 weeks into the experiment, 70% (of 378 total) of salamander larvae died in 4 out of 6 ponds. Native salamanders survived (n = 12) in these ponds only if they had metamorphosed prior to the die‐offs. During die‐offs, all larvae of native salamanders died, whereas 56% of hybrid larvae died. We necropsied native and hybrid salamanders, tested water quality, and queried the California Department of Pesticide Regulation database to investigate possible causes of the die‐offs. Salamander die‐offs, changes in the abundance of other community members (invertebrates, algae, and cyanobacteria), shifts in salamander sex ratio, and patterns of pesticide application in adjacent fields suggest that pesticide use may have contributed to die‐offs. That all survivors were hybrids suggests that environmental stress may promote rapid displacement of native genotypes. Efectos Letales de la Calidad del Agua sobre Salamandras de California Amenazadas pero no sobre Salamandras Híbridas Concurrentes     

中国西南不同湿地类型水鸟多样性评价

水鸟是反映湿地生态系统健康的指标之一,对不同湿地水鸟多样性进行评价能提出有利于湿地生态系统健康良性发展的湿地管理措施,亦能更好地保护水鸟资源。为缓解近年来中国自然湿地退化和消失的问题,中国政府积极推进实施了大量修复自然湿地和新建人工湿地的工程。然而受限于人力、物力和经费,当前对自然湿地和人工湿地水鸟多样性的系统调查和评价多局限于单一地点或局部区域。因水鸟的分布具有地域性,从地域尺度进行不同湿地类型水鸟多样性评价将更为客观,公众科学数据为区域大尺度数据获取提供了可能。本研究借助中国观鸟记录中心数据,比较了2014年1月至2018年2月中国西南4省(市)自然湿地和人工湿地的水鸟物种多样性和组成相似性;结果共记录到9目19科139种水鸟,其中有94.96%的物种分布于自然湿地,67.63%的物种分布于人工湿地;各类群水鸟在自然湿地中的物种数均高于人工湿地。结果说明自然湿地在水鸟保护中具有重要栖息地功能,人工湿地能为大部分水鸟提供栖息地,但不能完全代替自然湿地。自然湿地中的湖泊湿地、河流湿地和沼泽化草甸以及人工湿地中的水库、城市人工景观水面和娱乐水面是水鸟,特别是受威胁水鸟分布的重要湿地类型;建议政府部门重点对上述湿地亚类进行合理的规划和管理。     

Effects of the Interaction between Genetic Diversity and UV-B Radiation on Wood Frog Fitness

Abstract:  Genetic diversity may buffer amphibian populations against environmental vicissitudes. We hypothesized that wood frogs (  Rana sylvatica ) from populations with lower genetic diversity are more susceptible to ultraviolet-B (UV-B) radiation than those from populations with higher diversity. We used RAPD markers to obtain genetic diversity estimates for 12 wood frog populations. We reared larval wood frogs from these populations and exposed experimental groups of eggs and larvae to one of three treatments: unfiltered sunlight, sunlight filtered through a UV-B-blocking filter (Mylar), and sunlight filtered through a UV-B-transmitting filter (acetate). In groups exposed to UV-B, larval mortality and deformity rates increased significantly, but egg mortality did not. We found a significant negative relationship between genetic diversity and egg mortality, larval mortality, and deformity rates. Furthermore, the interaction between UV-B treatment and genetic diversity significantly affected larval mortality. Populations with low genetic diversity experienced higher larval mortality rates when exposed to UV-B than did populations with high genetic diversity. This is the first time an interaction between genetic diversity and an environmental stressor has been documented in amphibians. Differences in genetic diversity among populations, coupled with environmental stressors, may help explain patterns of amphibian decline.     

A Matrix‐Calibrated Species‐Area Model for Predicting Biodiversity Losses Due to Land‐Use Change

Abstract: Application of island biogeography theory to prediction of species extinctions resulting from habitat loss is based on the assumption that the transformed landscape matrix is completely inhospitable to the taxa considered, despite evidence demonstrating the nontrivial influence of matrix on populations within habitat remnants. The island biogeography paradigm therefore needs refining to account for specific responses of taxa to the area of habitat “islands” and to the quality of the surrounding matrix. We incorporated matrix effects into island theory by partitioning the slope (z value) of species–area relationships into two components: γ, a constant, and σ, a measure of taxon‐specific responses to each component of a heterogeneous matrix. We used our matrix‐calibrated model to predict extinction and endangerment of bird species resulting from land‐use change in 20 biodiversity hotspots and compared these predictions with observed numbers of extinct and threatened bird species. We repeated this analysis with the conventional species–area model and the countryside species–area model, considering alternative z values of 0.35 (island) or 0.22 (continental). We evaluated the relative strength of support for each of the five candidate models with Akaike's information criterion (AIC). The matrix‐calibrated model had the highest AIC weight (w_i = 89.21%), which means the weight of evidence in support of this model was the optimal model given the set of candidate models and the data. In addition to being a valuable heuristic tool for assessing extinction risk, our matrix‐calibrated model also allows quantitative assessment of biodiversity benefits (and trade‐offs) of land‐management options in human‐dominated landscapes. Given that processes of secondary regeneration have become more widespread across tropical regions and are predicted to increase, our matrix‐calibrated model will be increasingly appropriate for practical conservation in tropical landscapes.     

Assessing Changes in Amphibian Population Dynamics Following Experimental Manipulations of Introduced Fish

Abstract: Sport‐fish introductions are now recognized as an important cause of amphibian decline, but few researchers have quantified the demographic responses of amphibians to current options in fisheries management designed to minimize effects on sensitive amphibians. Demographic analyses with mark–recapture data allow researchers to assess the relative importance of survival, local recruitment, and migration to changes in population densities. I conducted a 4‐year, replicated whole‐lake experiment in the Klamath Mountains of northern California (U.S.A.) to quantify changes in population density, survival, population growth rate, and recruitment of the Cascades frog (Rana cascadae) in response to manipulations of non‐native fish populations. I compared responses of the frogs in lakes where fish were removed, in lakes in their naturally fish‐free state, and in lakes where fish remained that were either stocked annually or no longer being stocked. Within 3 years of fish removals from 3 lakes, frog densities increased by a factor of 13.6. The survival of young adult frogs increased from 59% to 94%, and realized population growth and recruitment rates at the fish‐removal lakes were more than twice as high as the rates for fish‐free reference lakes and lakes that contained fish. Population growth in the fish‐removal lakes was likely due to better on‐site recruitment of frogs to later life stages rather than increased immigration. The effects on R. cascadae of suspending stocking were ambiguous and suggested no direct benefit to amphibians. With amphibians declining worldwide, these results show that active restoration can slow or reverse the decline of species affected by fish stocking within a short time frame.     

Local Land‐Use Planning to Conserve Biodiversity: Planners’ Perspectives on What Works

Abstract: Because habitat loss due to urbanization is a primary threat to biodiversity, and land‐use decisions in urbanizing areas are mainly made at the local level, land‐use planning by municipal planning departments has a potentially important—but largely unrealized—role in conserving biodiversity. To understand planners’ perspectives on the factors that facilitate and impede biodiversity conservation in local planning, we interviewed directors of 17 municipal planning departments in the greater Seattle (Washington, U.S.A.) area and compared responses of planners from similar‐sized jurisdictions that were “high” and “low performing” with respect to incorporation of biodiversity conservation in local planning. Planners from low‐performing jurisdictions regarded mandates from higher governmental levels as the primary drivers of biodiversity conservation, whereas those from high‐performing jurisdictions regarded community values as the main drivers, although they also indicated that mandates were important. Biodiversity conservation was associated with presence of local conservation flagship elements (e.g., salmonids) and human‐centered benefits of biodiversity conservation (e.g., quality of life). Planners from high‐ and low‐performing jurisdictions favored different planning mechanisms for biodiversity conservation, perhaps reflecting differences in funding and staffing. High performers reported more collaborations with other entities on biodiversity issues. Planners’ comments indicated that the term biodiversity may be problematic in the context of local planning. The action most planners recommended to increase biodiversity conservation in local planning was public education. These results suggest that to advance biodiversity conservation in local land‐use planning, conservation biologists should investigate and educate the public about local conservation flagships and human benefits of local biodiversity, work to raise ecological literacy and explain biodiversity more effectively to the public, and promote collaboration on biodiversity conservation among jurisdictions and inclusion of biodiversity specialists in planning departments.     

Importance of Habitat Quality and Landscape Connectivity for the Persistence of Endangered Natterjack Toads

Abstract: The natterjack toad (Bufo calamita) is endangered in several parts of its distribution, including Belgium, where it occurs mainly in artificial habitats. We parameterized a general model for natterjack population viability analysis (PVA) and tested its sensitivity to changes in the values of basic parameters. Then we assessed the relative efficiency of various conservation measures in 2 situations: a small isolated population and a system of 4 populations connected by rare dispersal movements. We based the population viability analysis on a stage‐structured model of natterjack population dynamics. We parameterized the model in the RAMAS GIS platform with vital rates obtained from our own field experience and from published studies. Simulated natterjack populations were highly sensitive to habitat quality (particularly pond drying), to dispersal from surrounding local populations, and to a lesser extent to values of fecundity and survival of terrestrial stages. Population trajectories were nearly insensitive to initial abundances, carrying capacities, and the frequency of extreme climatic conditions. The simulations showed that in habitats with highly ephemeral ponds, where premetamorphosis mortality was high, natterjack populations nearly always had a very high extinction risk. We also illustrated how low dispersal rates (<1 dispersing individual/generation) efficiently rescued declining local populations. Such source‐sink dynamics demonstrate that the identification and management of source populations should be a high priority.