Visually determined stream mesohabitats influence benthic macroinvertebrate assessments in headwater streams

Mesohabitat components such as substrate and surface flow types are intimately related to benthic macroinvertebrate assemblages in streams. Visual assessments of the distribution of these components provide a means of evaluating physical habitat heterogeneity and aid biodiversity surveys and monitoring. We determined the degree to which stream site and visually assessed mesohabitat variables explain variability (i.e., beta-diversity) in the relative abundance and presence-absence of all macroinvertebrate families and of Ephemeroptera, Plecoptera, and Trichoptera (EPT) genera. We systematically sampled a wide variety of mesohabitat arrangements as they occured in stream sites. We also estimated how much of the explanation given by mesohabitat was associated with substrate or surface flow types. We performed variation partitioning to determine fractions of explained variance through use of partial redundancy analysis (pRDA). Mesohabitats and stream sites explained together from 23 to 32 % of the variation in the four analyses. Stream site explained 8–11 % of that variation, and mesohabitat variables explained 13–20 %. Surface flow types accounted for >60 % of the variation provided by the mesohabitat component. These patterns are in accordance with those obtained in previous studies that showed the predominance of environmental variables over spatial location in explaining macroinvertebrate distribution. We conclude that visually assessed mesohabitat components are important predictors of assemblage composition, explaining significant amounts of beta-diversity. Therefore, they are critical to consider in ecological and biodiversity assessments involving macroinvertebrates.     

Relationships among varying sampling distance and the IBI in warmwater,headwater streams of the Eastern Corn Belt Plain

Single-pass electrofishing was used to define the most efficient sampling distance to assess stream condition using the index of biotic integrity (IBI) methodology in headwater (<36 km² drainage area), warmwater streams in the Eastern Corn Belt Plain ecoregion. Based on wetted widths (1–3.3 m) of sampled reaches, we defined effort based on increased area (range 50–555 m²). Sampled area necessary to capture a representative fish assemblage increased until 167-m² distance, which is equivalent to a minimum sampling distance of one habitat cycle. No significant difference in metric actual observed value response was found with increasing habitat cycle. Increased effort is required in smaller streams widths (≤1 m) to achieve the recommended sample area. The effect of rare fish on the IBI was tested using a modified Walford method. A significant decrease in IBI score was observed when 10 % of the rare data were removed. The presence of rare fish did not influence individual IBI metrics or scores for either the increased effort or reduced effort calibrations until greater than 3 % of the data was removed for number of species, 15 % removal of data for number of minnow species, and 5 % removal of data for catch per unit effort (CPUE). Increased effort did not affect any metric or IBI score, while reduced effort influenced the number of darter, madtom, and sculpin species and catch per unit effort metric scores but did not affect IBI score.     

Evaluation of an alternate method for sampling benthic macroinvertebrates in low-gradient streams sampled as part of the National Rivers and Streams Assessment

Benthic macroinvertebrates are sampled in streams and rivers as one of the assessment elements of the US Environmental Protection Agency’s National Rivers and Streams Assessment. In a 2006 report, the recommendation was made that different yet comparable methods be evaluated for different types of streams (e.g., low gradient vs. high gradient). Consequently, a research element was added to the 2008–2009 National Rivers and Streams Assessment to conduct a side-by-side comparison of the standard macroinvertebrate sampling method with an alternate method specifically designed for low-gradient wadeable streams and rivers that focused more on stream edge habitat. Samples were collected using each method at 525 sites in five of nine aggregate ecoregions located in the conterminous USA. Methods were compared using the benthic macroinvertebrate multimetric index developed for the 2006 Wadeable Streams Assessment. Statistical analysis did not reveal any trends that would suggest the overall assessment of low-gradient streams on a regional or national scale would change if the alternate method was used rather than the standard sampling method, regardless of the gradient cutoff used to define low-gradient streams. Based on these results, the National Rivers and Streams Survey should continue to use the standard field method for sampling all streams.     

Monitoring fish communities in wadeable lowland streams: comparing the efficiency of electrofishing methods at contrasting fish assemblages

Electrofishing is considered a reliable tool to assess the assemblages and biodiversity of fish in wadeable streams. The most widely used electrofishing techniques (point [P], single-pass [S-P], and multiple-pass [M-P]) vary as to the effort needed for sample collection, and this may potentially influence the degree of accuracy. Moreover, little is known about the comparability of the methods and their specific performance in streams with different fish assemblages. The aim of this investigation was to validate (using M-P sampling as reference) the use of P and S-P electrofishing techniques to accurately assess the richness, density and size distribution of fishes in small streams at both regional and global scale independently of fish assemblages and geographical region. We sampled 50-m-long reaches in a total of 33 lowland stream reaches that were located in different climatic and biogeographical regions (Uruguay and Denmark) and hosted different fish assemblages. Subtropical fish communities exhibited higher richness (Uy: 12–32, Dk: 1–9) and densities (Uy: 1.3–5.2, DK: 0.1–4.9 in. m^?2) than temperate streams. We applied both "global models" using the entire database (33 sites) and "local models" including the same number of sites but using the climatic region as a model variable. Regression analyses revealed that the P, S-P and M-P methods all provided an adequate picture of the species composition and size distribution, and transfer equations for comparison between methods are thus not required. Conversely, richness was better predicted by S-P and by P techniques for regional and global models, respectively. Transfer equations obtained for abundance revealed that the P and S-P models can accurately transform catch data into M-P estimations. The transfer equations provided here may have great relevance as they allow relatively reliable comparisons to be made between data obtained by different techniques. We also show that less intensive sampling techniques may be equally useful for monitoring purposes as those requiring more intensive efforts (and costs). We encourage validation of our developed transfer equations on data from other regions of the world.     

Determining subsampling effort for the development of a rapid bioassessment protocol using benthic macroinvertebrates in streams of Southeastern Brazil

The purpose of this study was to establish a subsampling procedure for benthic macroinvertebrates to aid in the development of a multimetric index to assess the biological condition of streams. Data from six streams that are considered minimally impaired were used. Subsampling was done using a device divided into 24 quadrats. The sediment from each quadrat was sorted, and all organisms were removed and identified. Richness metrics were the most affected by subsample size. Relative-abundance metrics were the most stable, proving that the sample was well distributed throughout the tray and abundance proportions were maintained. The results showed that the macroinvertebrate assemblage present in the six quadrats was similar to that present in the full sample. These analyses indicated that six quadrats, randomly designated, with a minimum of 200 collected specimens could be used in place of the full sample. In routine water management, managing time and costs are a major challenge; therefore, this type of simplification is absolutely necessary to ensure that a biomonitoring tool is useful for practical applications.     

Quantifying the sensitivity of ephemeral streams to land disturbance activities in arid ecosystems at the watershed scale

Large areas of public lands administered by the Bureau of Land Management and located in arid regions of the southwestern United States are being considered for the development of utility-scale solar energy facilities. Land-disturbing activities in these desert, alluvium-filled valleys have the potential to adversely affect the hydrologic and ecologic functions of ephemeral streams. Regulation and management of ephemeral streams typically falls under a spectrum of federal, state, and local programs, but scientifically based guidelines for protecting ephemeral streams with respect to land-development activities are largely nonexistent. This study developed an assessment approach for quantifying the sensitivity to land disturbance of ephemeral stream reaches located in proposed solar energy zones (SEZs). The ephemeral stream assessment approach used publicly-available geospatial data on hydrology, topography, surficial geology, and soil characteristics, as well as high-resolution aerial imagery. These datasets were used to inform a professional judgment-based score index of potential land disturbance impacts on selected critical functions of ephemeral streams, including flow and sediment conveyance, ecological habitat value, and groundwater recharge. The total sensitivity scores (sum of scores for the critical stream functions of flow and sediment conveyance, ecological habitats, and groundwater recharge) were used to identify highly sensitive stream reaches to inform decisions on developable areas in SEZs. Total sensitivity scores typically reflected the scores of the individual stream functions; some exceptions pertain to groundwater recharge and ecological habitats. The primary limitations of this assessment approach were the lack of high-resolution identification of ephemeral stream channels in the existing National Hydrography Dataset, and the lack of mechanistic processes describing potential impacts on ephemeral stream functions at the watershed scale. The primary strength of this assessment approach is that it allows watershed-scale planning for low-impact development in arid ecosystems; the qualitative scoring of potential impacts can also be adjusted to accommodate new geospatial data, and to allow for expert and stakeholder input into decisions regarding the identification and potential avoidance of highly sensitive stream reaches.     

A longitudinal assessment of the aquatic macroinvertebrate community in the channelized lower Missouri River

We conducted an aquatic macroinvertebrate assessment in the channelized reach of the lower Missouri River, and used statistical analysis of individual metrics and multimetric scores to identify community response patterns and evaluate relative biological condition. We examined longitudinal site differences that are potentially associated with water qualityrelated factors originating from the Kansas City metropolitan area, using data from coarse rock substrate in flowing water habitats (outside river bends), and depositional mud substratein slack water habitats (dike fields). Three sites above rivermile (RM) 369 in Kansas City (Nebraska City, RM = 560; St. Joseph, RM = 530; Parkville, RM = 377) and three below (Lexington, RM = 319; Glasgow, RM = 228; Hermann, RM = 94) were sampled with rock basket artificial substrates, a qualitative kicknet method, and the Petite Ponar. We also compared the performance of the methods used. A total of 132 aquatic macroinvertebrate taxa were collected from the lower Missouri River; one third of these taxa belonged to the sensitiveEPOT insect orders (Ephemeroptera, Plecoptera, Odonata, and Trichoptera). Rock baskets had the highest mean efficiency (34.1%) of the methods, and the largest number of taxa was collected by Ponar (n = 69) and kicknet (n = 69) methods. Seven of the 15 metrics calculated from rock basket data, and five ofthe nine metrics calculated from Ponar data showed highly significant differences (ANOVA, P < 0.001) at one or more sitesbelow Kansas City. We observed a substantial reduction in net-spinning Trichoptera in rock habitats below Kansas City (Lexington), an increase in relative dominance of Oligochaeta in depositional habitats at the next site downstream (Glasgow), and lower relative condition scores in rock habitat at Lexingtonand depositional habitat at Glasgow. Collectively, these data indicate that some urban-related impacts on the aquatic macroinvertebrate community are occurring. Our results suggest that the methods and assessment framework we used in this studycould be successfully applied on a larger scale with concurrentwater and sediment chemistry to validate metrics, establish impairment levels, and develop a specific macroinvertebrate community index for the lower Missouri River. We recommend accomplishing this with longitudinal multi-habitat sampling at a larger number of sites related to all potential sources of impairment, including major tributaries, urban areas, and point sources.     

Spatial scales and probability based sampling in determining levels of benthic community degradation in the Chesapeake Bay

The extent of degradation of benthic communities of the Chesapeake Bay was determined by applying a previously developed benthic index of biotic integrity at three spatial scales. Allocation of sampling was probability-based allowing areal estimates of degradation with known confidence intervals. The three spatial scales were: (1) the tidal Chesapeake Bay; (2) the Elizabeth River watershed; and (3) two small tidal creeks within the Southern Branch of the Elizabeth River that are part of a sediment contaminant remediation effort. The areas covered varied from 10^–1 to 10⁴ km² and all were sampled in 1999. The Chesapeake Bay was divided into ten strata, the Elizabeth River into five strata and each of the two tidal creeks was a single stratum. The determination of the number and size of strata was based upon consideration of both managerially useful units for restoration and limitations of funding. Within each stratum 25 random locations were sampled for benthic community condition. In 1999 the percent of the benthos with poor benthic community condition for the entire Chesapeake Bay was 47% and varied from 20% at the mouth of the Bay to 72% in the Potomac River. The estimated area of benthos with poor benthic community condition for the Elizabeth River was 64% and varied from 52–92%. Both small tidal creeks had estimates of 76% of poor benthic community condition. These kinds of estimates allow environmental managers to better direct restoration efforts and evaluate progress towards restoration. Patterns of benthic community condition at smaller spatial scales may not be correctly inferred from larger spatial scales. Comparisons of patterns in benthic community condition across spatial scales, and between combinations of strata, must be cautiously interpreted.     

Bioassessment tools in novel habitats: an evaluation of indices and sampling methods in low-gradient streams in California

Biomonitoring programs are often required to assess streams for which assessment tools have not been developed. For example, low-gradient streams (slope ≤1%) comprise 20–30% of stream miles in California and are of particular interest to watershed managers, yet most sampling methods and bioassessment indices in the state were developed in high-gradient systems. This study evaluated the performance of three sampling methods [targeted riffle composite (TRC), reach-wide benthos (RWB), and the margin–center–margin modification of RWB (MCM)] and two indices [the Southern California Index of Biotic Integrity (SCIBI) and the ratio of observed to expected taxa (O/E)] in low-gradient streams in California for application in this habitat type. Performance was evaluated in terms of efficacy (i.e., ability to collect enough individuals for index calculation), comparability (i.e., similarity of assemblages and index scores), sensitivity (i.e., responsiveness to disturbance), and precision (i.e., ability to detect small differences in index scores). The sampling methods varied in the degree to which they targeted macroinvertebrate-rich microhabitats, such as riffles and vegetated margins, which may be naturally scarce in low-gradient streams. The RWB method failed to collect sufficient numbers of individuals (i.e., ≥450) to calculate the SCIBI in 28 of 45 samples and often collected fewer than 100 individuals, suggesting it is inappropriate for low-gradient streams in California; failures for the other methods were less common (TRC, 16 samples; MCM, 11 samples). Within-site precision, measured as the minimum detectable difference (MDD) was poor but similar across methods for the SCIBI (ranging from 19 to 22). However, RWB had the lowest MDD for O/E scores (0.20 versus 0.24 and 0.28 for MCM and TRC, respectively). Mantel correlations showed that assemblages were more similar within sites among methods than within methods among sites, suggesting that the sampling methods were collecting similar assemblages of organisms. Statistically significant disagreements among methods were not detected, although O/E scores were higher for RWB samples than TRC. Index scores suggested impairment at all sites in the study. Although index scores did not respond strongly to several measurements of disturbance in the watershed, percent agriculture showed a significant, negative relationship with O/E scores.     

Application of a benthic observed/expected-type model for assessing Central Appalachian streams influenced by regional stressors in West Virginia and Kentucky

Stream bioassessments rely on taxonomic composition at sites compared with natural, reference conditions. We developed and tested an observed/expected (O/E) predictive model of taxonomic completeness and an index of compositional dissimilarity (BC index) for Central Appalachian streams using combined macroinvertebrate datasets from riffle habitats in West Virginia (WV) and Kentucky (KY). A total of 102 reference sites were used to calibrate the O/E model, which was then applied to assess over 1,200 sites sampled over a 10-year period. Using an all subsets discriminant function analysis (DFA) procedure, we tested combinations of 14 predictor variables that produced DF and O/E models of varying performance. We selected the most precise model using a probability of capture at >0.5 (O/E _0.5, SD?=?0.159); this model was constructed with only three simple predictor variables—Julian day, latitude, and whether a site was in ecoregion 69a. We evaluated O/E and BC indices between reference and test sites and compared their response to regional stressors, including coal mining, residential development, and acid deposition. The Central Appalachian O/E and BC indices both showed excellent discriminatory power and were significantly correlated to a variety of regional stressors; in some instances, the BC index was slightly more sensitive and responsive than the O/E _0.5 model. These indices can be used to supplement existing bioassessment tools crucial to detecting and diagnosing stream impacts in the Central Appalachian region of WV and KY.     

Assessing differences in macrofaunal assemblages as a factor of sieve mesh size,distance between samples,and time of sampling

With increasing cascading effects of climate change on the marine environment, as well as pollution and anthropogenic utilization of the seafloor, there is increasing interest in tracking changes to benthic communities. Macrofaunal surveys are traditionally conducted as part of pre-incident environmental assessment studies and post-incident monitoring studies when there is a potential impact to the seafloor. These surveys usually characterize the structure and/or spatiotemporal distribution of macrofaunal assemblages collected with sediment cores; however, many different sampling protocols have been used. An assessment of the comparability of past and current survey methods was in need to facilitate future surveys and comparisons. This was the aim of the present study, conducted off the Oregon coast in waters 25–35 m deep. Our results show that the use of a sieve with a 1.0-mm mesh size gives results for community structure comparable to results obtained from a 0.5-mm mesh size, which allows reliable comparisons of recent and past spatiotemporal surveys of macroinfauna. In addition to our primary objective of comparing methods, we also found interacting effects of seasons and depths of collection. Seasonal differences (summer and fall) were seen in infaunal assemblages in the wave-induced sediment motion zone but not deeper. Thus, studies where wave-induced sediment motion can structure the benthic communities, especially during the winter months, should consider this effect when making temporal comparisons. In addition, some macrofauna taxa-like polychaetes and amphipods show high interannual variabilities, so spatiotemporal studies should make sure to cover several years before drawing any conclusions.     

Variability in the identification and enumeration of marine benthic invertebrate samples and its effect on benthic assessment measures

Studies designed to measure anthropogenic impacts on marine benthic communities depend on the ability of taxonomists to consistently discriminate, identify, and count benthic organisms. To quantify errors and discrepancies in identification and enumeration, 20 samples were completely reprocessed by another one of four participating laboratories. Errors were detected in 13.0% of the data records, affecting total abundance by 2.1%, numbers of taxa by 3.4%, and identification accuracy by 4.7%. Paired t-tests were used to test for differences in the Benthic Response Index (BRI), total abundance, numbers of taxa, and the Shannon-Wiener index between the original and the reanalysis data. Differences in the BRI were statistically insignificant. Although statistically significant differences were observed for numbers of taxa, total abundance, and the Shannon-Wiener index, the differences were small in comparison to the magnitude of differences typically observed between anthropogenically affected and reference sites.     

Low-flow active and passive sampling of VOCs using thermal desorption tubes: theory and application at an offset printing facility

While air sampling techniques using adsorbent-based collection, thermal desorption and chromatographic analysis have found a niche in ambient air sampling, occupational applications have been more limited. This paper evaluates the use of thermal desorption techniques for low flow active and passive sampling configurations which allow conveniently long duration sampling in occupational settings and other high concentration environments. The use of an orifice enables flows as low as 0.5 ml min(-1) and sampling periods up to several days without significant biases. A model is used to predict sampling rates of a passive sampler encompassing an orifice, a void space, glass wool, and the adsorbent. Laboratory and field tests conducted at a commercial offset printing facility, which contained a variety of volatile organic compounds (primarily aromatic but also a few chlorinated and terpene compounds at levels from 1 to 67,000 microg m(-3)), are used to evaluate the approach. Tenax GR and Carbosieve SIII, both singly and together, were employed as adsorbents. Side-by-side tests comparing high flow, low flow and passive samplers show excellent agreement and high linearity (r = 0.95) for concentrations spanning nearly five orders of magnitude. Active samplers were tested at flows as low as 0.5 ml min(-1), compared to typical flows up to 40 ml min(-1). Passive samplers demonstrated a linear range and agreement with predictions for adsorbate loadings from approximately 1 ng to nearly 10 microg. Using a chemical mass balance receptor model, concentrations in the facility were apportioned to solvents, inks and other indoor and outdoor sources. Overall, the use of low flow active and passive sampling approaches employing thermal desorption techniques provides good performance and tremendous flexibility that facilitates use in many applications, including workplace settings.     

The effects of subsampling and sampling frequency on the use of surface-floating pupal exuviae to measure Chironomidae (Diptera) communities in wadeable temperate streams

Community, diversity, and biological index metrics for chironomid surface-floating pupal exuviae (SFPE) were assessed at different subsample sizes and sampling frequencies from wadeable streams in Minnesota (USA). Timed collections of SFPE were made using a biweekly sampling interval in groundwater-dominated (GWD) and surface-water-dominated (SWD) streams. These two types of stream were sampled because they support different Chironomidae communities with different phenologies which could necessitate sampling methodologies specific to each stream type. A subsample size of 300 individuals was sufficient to collect on average 85% of total taxa richness and to estimate most metrics with an error of about 1% relative to 1,000 count samples. SWD streams required larger subsample sizes to achieve similar estimates of taxa richness and metric error compared to GWD streams, but these differences were not large enough to recommend different subsampling methods for these stream types. Analysis of sample timing determined that 97% of emergence occurred from April through September. We recommend in studies where estimation of winter emergence is not important that sampling be limited to this period. Sampling frequency also affected the proportion of the community collected. To maximize the portion of the community, collected samples should be taken across seasons although no specific sampling interval is recommended. Subsampling and sampling frequency was also assessed simultaneously. When using a 300-count subsample, a 4-week sampling interval from April through September was required to collect on average 71% of the community. Due to differences in elements of the chironomid community evaluated by different studies (e.g., biological condition, phenology, and taxonomic composition), richness estimates are documented for five sampling intervals (2, 4, 6, 8, 10, and 12 weeks) and five subsample sizes (100, 200, 300, 500, and 1,000 counts). This research will enhance future studies by providing guidelines for tailoring SFPE methods to study specific goals and resources.     

Assessing the ecological condition of streams in a southeastern Brazilian basin using a probabilistic monitoring design

Prompt assessment and management actions are required if we are to reduce the current rapid loss of habitat and biodiversity worldwide. Statistically valid quantification of the biota and habitat condition in water bodies are prerequisites for rigorous assessment of aquatic biodiversity and habitat. We assessed the ecological condition of streams in a southeastern Brazilian basin. We quantified the percentage of stream length in good, fair, and poor ecological condition according to benthic macroinvertebrate assemblage. We assessed the risk of finding degraded ecological condition associated with degraded aquatic riparian physical habitat condition, watershed condition, and water quality. We describe field sampling and implementation issues encountered in our survey and discuss design options to remedy them. Survey sample sites were selected using a spatially balanced, stratified random design, which enabled us to put confidence bounds on the ecological condition estimates derived from the stream survey. The benthic condition index indicated that 62 % of stream length in the basin was in poor ecological condition, and 13 % of stream length was in fair condition. The risk of finding degraded biological condition when the riparian vegetation and forests in upstream catchments were degraded was 2.5 and 4 times higher, compared to streams rated as good for the same stressors. We demonstrated that the GRTS statistical sampling method can be used routinely in Brazilian rain forests and other South American regions with similar conditions. This survey establishes an initial baseline for monitoring the condition and trends of streams in the region.     

Environmental assessment of agriculture at a regional scale in the Pampas of Argentina

Governments need good information to design policies. However, inthe Argentine Pampas there are neither sufficient knowledge on environmental issues, nor clear perception of environmental alterations across space and time. The general objective of this work was to provide decision makers with a scientifically sound set of indicators aiming at the assessment of current status andfuture trends in the rural environment of this sensitiveregion. As driving criteria to select indicators, weassumed that they had to be sound, simple to calculate,easy to understand, and easily applicable by decision makers. They are related closely to significantecological structures and functions. Twelve basicindicators were identified: (1) land use, (2) fossil energyuse, (3) fossil energy use efficiency, (4) nitrogen (N)balance, (5) phosphorus (P) balance, (6) nitrogencontamination risk, (7) phosphorus contamination risk, (8) pesticide contamination, (9) soil erosion risk, (10) habitatintervention, (11) changes in soil carbon stock, and (12) balance of greenhouse gases. Indicators were geographicallyreferenced using a geographic information system (GIS). Thestrength of this study is not in the absolute value ofenvironmental indicators, but rather in theconceptualization of indicator and the identification ofchanging patterns, gradients and trends in space and time.According to our results, we can not definitely say thatagriculture in the Pampas, as a whole, tends to besustainable or not. While some indicators tend to improve,others keep stable, and the rest worsen. The relative importance among indicators must also be considered. The indicators that showed a negative net change are key to the identification of critical problems that will require special attention in the close future.     

Chemical and biological characteristics of streams in the Owabi watershed

In this investigation, concentrations of physico-chemical and bacteriological qualities of water samples from the major streams within the Owabi watershed in Kumasi, Ghana, were measured at five different locations. The streams were moderately soft and neutral, having a mean pH range of 7.08 ± 0.2 to 7.88 ± 0.6. Total dissolved solids, total suspended solids, grease and oil, alkalinity, and the major ion levels varied significantly at each sampling site. Nutrient levels were however low and did not show any clear variation at sample locations. The bacteriological quality of the water was poor, rendering it unsafe for domestic purposes without treatment. The poor bacteriological quality was due to direct contamination by animal and human wastes. The streams have an appreciable self-purification capacity which is stressed by persistent pollution overloads caused by expanding human activities within the catchment. Cluster analysis performed on the data to determine pollution patterns between the streams depicts that River Owabi was less polluted, Rivers Akyeampomene and Sukobri were moderately polluted, while River Pumpunase was highly polluted.     

Assessing the potentials of Lemna minor in the treatment of domestic wastewater at pilot scale

Water crisis is one of the most serious problems faced by the world today. Phytoremediation is one of the serious efforts towards sustainability. Macrophyte-based wastewater treatment systems have several potential advantages compared with conventional treatment systems. Duckweeds (Lemna spp., Spirodela spp., Wolffia spp.) are small, green freshwater, free-floating aquatic plants. The primary objective of this work was to analyze the role of duckweeds in organic waste and nutrient removal from domestic wastewater being generated from hostels of Birla Institute of Technology, Mesra, Ranchi (India). Interesting results were obtained in which the BOD value reduced by 94.45% and the level of orthophosphate at the end of the work was found to be reduced by 79.39%. The duckweeds flourished well during the experimental period in the pH range of 7 to 8; it can be said that, other factors remaining favorable, the optimum pH for duckweed growth ranges from 7 to 8. Therefore, it can be concluded that this treatment can be successfully carried out on a large scale. Also, it is a low-cost solution to wastewater treatment problems and could satisfy the discharge standards.