Biomarkers as tools to assess the chronic toxicity of ammonia in the juvenile Mugil cephalus

With juvenile fish as the subject, the effects of low concentration ammonia on antioxidant system were studied using Mugil cephalus. Samples of gill and liver tissue were obtained from 0.35, 0.70, 1.5 and 3?mg/L ammonia groups at 0, 5, 10, 15 and 20 days of exposure, at which times the biomarkers were measured. Results showed that gill malondialdehyde (MDA) content exhibited an initial significant increase (p?≤?0.05) at unionised ammonia concentrations of 0.70, 1.5 and 3.0?mg/L on day 5, followed by subsequent declines, while liver MDA levels exhibited significant increases (p?≤?0.05) at unionised ammonia concentrations of 1.5?mg/L starting on day 10 and at 3.0?mg/L starting on day 5. With exposure to ammonia at different concentrations, Na⁺-K⁺-ATPase activity in liver and gill decreased over time. The Na⁺-K⁺-ATPase activity was negatively related to ammonia concentration from 0.70 to 3.0?mg/L. Overall, our results show that MDA and Na⁺-K⁺-ATPase, evaluated here as potential biomarkers of ammonia exposure, exhibited responses to sublethal concentrations of ammonia that were concentration dependent.     

Participatory tools for coastal zone management: Use of stakeholder analysis and social mapping in Australia

This paper presents research currently being conducted in Central Queensland, Australia to understand conflicts between coastal zone resource users and the associated sociocultural and political issues surrounding coastal zone management. Conflict occurs between stakeholders in the coastal zone over values, conservation and development trade-offs, access, and resource use rights. Decisions are currently made within a multi-stakeholder framework where there is limited understanding among stakeholders of each groups values and aspirations, and few, mechanisms for negotiation, or to ensure transparency of decisions and feedback on consultation. This paper reports on the contribution of stakeholder analysis and social mapping to conflict management and findings from their application. As it is applied here, stakeholder analysis and social mapping have been successful participatory tools used to document and feed back the values, interests, attitudes and aspirations of stakeholders. Understanding stakeholder conflict is essential in progressing a whole catchment approach to decision-making that secures the cooperation of a diverse range of social groups.     

Using the paired comparison methodology to assess environmental values in the coastal zone of Ghana

Like many developing countries, Ghana is grappling with environmental issues, such as the degradation of coastal natural resources. In an attempt to ameliorate the current state of affairs, broad institutional arrangements and policy interventions have been put in place. However coastal natural resources are still being overexploited and the effects of this are felt more strongly by certain groups (such as women and the rural poor) than others. This has led to the argument pointing to an urgent need to include local resource users as active participants in environmental decision making and to incorporate their environmental values in the management of coastal natural resources. Using empirical data from women in two Ghanaian coastal communities, this paper highlights the importance of exploring how the environmental attitudes and values of women might be considered in the management of local coastal natural resources. It assesses effectiveness of the paired comparison methodology in this respects and how this method can be also used to prioritise their environmental concerns. The results show that in both areas respondents place the greatest importance on the coastal natural resources as sources of wealth creation and of food, values which are often described as being “anthropocentric”. The paper thus argues that having discovered that the ecological value placed on natural resources is low, initiatives that could win the support of people would be those that link the livelihood and wellbeing to coastal NRM. It emphasizes the fact that conservation cannot exist outside of people and social systems; nor will it succeed unless they are linked to the welfare of resource users.     

Biological benthic tools as indicators of coastal marine ecosystems health

Coastal marine ecosystems are increasingly subjected to environmental stress and degradation due to pollution. Several research programmes have addressed this problem and produced relevant data sets for specific areas, often including consistent sets of environmental and biological variables. The value of existing information gathered from these types of data can be largely increased by combining them into a common data set to determine globally applicable relationships. To perform this exercise, the Intergovernmental Oceanographic Commission (IOC) of UNESCO has recently formed the Ad hoc Study Group on Benthic Indicators (http://www.ioc.unesco.org/benthicindicators) with the aim of developing robust indicators of benthic health. In this paper, initial products and ongoing activities of this international initiative are described and discussed. An expansion of initial IOC/UNESCO research on benthic fauna-organic carbon relationships is also presented. As part of this follow-up research, the relationship between total organic carbon concentrations of sediment and abundance, biomass and species diversity of benthic macrofauna was evaluated using data sets from 2 different regions of the world comprising 3 different coastal marine environments. The ability of identifying threshold levels in selected variables that could serve as indicators of related adverse environmental conditions leading to stress in the benthos is envisaged within the frame of a larger joint analysis, carried out by the IOC/UNESCO Study Group on Benthic Indicators, of merged data sets from several coastal regions worldwide.     

Biological benthic tools as indicators of coastal marine ecosystems health

Coastal marine ecosystems are increasingly subjected to environmental stress and degradation due to pollution. Several research programmes have addressed this problem and produced relevant data sets for specific areas, often including consistent sets of environmental and biological variables. The value of existing information gathered from these types of data can be largely increased by combining them into a common data set to determine globally applicable relationships. To perform this exercise, the Intergovernmental Oceanographic Commission (IOC) of UNESCO has recently formed the Ad hoc Study Group on Benthic Indicators (http://www.ioc.unesco.org/benthicindicators) with the aim of developing robust indicators of benthic health. In this paper, initial products and ongoing activities of this international initiative are described and discussed. An expansion of initial IOC/UNESCO research on benthic fauna-organic carbon relationships is also presented. As part of this follow-up research, the relationship between total organic carbon concentrations of sediment and abundance, biomass and species diversity of benthic macrofauna was evaluated using data sets from 2 different regions of the world comprising 3 different coastal marine environments. The ability of identifying threshold levels in selected variables that could serve as indicators of related adverse environmental conditions leading to stress in the benthos is envisaged within the frame of a larger joint analysis, carried out by the IOC/UNESCO Study Group on Benthic Indicators, of merged data sets from several coastal regions worldwide.     

The foraging ecology of coastal bottlenose dolphins based on stable isotope mixing models and behavioural sampling

Understanding trophic interactions is critical for elucidating ecological roles of marine predators. We used behavioural observations and stable isotope mixing models to investigate the feeding ecology of Indo-Pacific bottlenose dolphins (Tursiops aduncus) in the lagoon of Mayotte (East Africa). We identified prey during 77 % of 54 observed feeding events, observed in both rainy (61 % of events) and dry (39 %) seasons. Caranx melampygus and Gnathanodon speciosus were involved in 67 % of these events, with Tylosurus crocodilus (20 %) and Mugil cephalus (13 %) also consumed. Mixing models, based on δ¹³C and δ¹⁵N values of skin and blubber (n = 30 samples for both tissues), suggest that behavioural observations are representative of general feeding patterns. Indeed, C. melampygus and T. crocodilus (G. speciosus could not be included in models) were estimated to contribute most to dolphin diets, with mean estimated contributions of 44.6 % (±18.9) and 48.1 % (±19.1) for skin and 73.7 % (±14.9) and 16.9 % (±12.4) for blubber, respectively. Our results highlight the value of two independent methods (stable isotopes and behavioural observations) to assess prey preferences of free-ranging dolphins.     

Winter diet shift of long-beaked common dolphins (Delphinus capensis) feeding in the sardine run off KwaZulu-Natal, South Africa

Unpredictable inter-annual variations in the timing, spatial extent and intensity of the sardine run (Sardinops sagax) have been documented in recent years along the coastline of KwaZulu-Natal, South Africa. This study aimed to determine whether variations in the availability of sardine were reflected in the diet and condition of common dolphins (Delphinus capensis) over the past three decades. Stomach contents from 95 common dolphins incidentally caught between 2000 and 2009 were analysed and compared to historical data collected between 1972 and 1992. A shift in the principal prey species consumed by the dolphins was observed over the past 30 years. Prior to 1992, sardine comprised up to 49 % of the total stomach contents by mass, whilst chub mackerel (Scomber japonicus) was the dominant prey recorded in the stomach contents between 2000 and 2009 (66 %). As common dolphins prey on locally abundant fish species, this dietary shift may indicate changes in the availability of the most abundant fish prey. Stable isotope analyses of tooth tissue revealed no significant changes in the δ¹⁵N and δ¹³C isotopic ratios over the past three decades (P = 0.283 for N and P = 0.922 for C). Blubber thickness as a percentage of body length and blubber weight as a percentage of total body weight were assessed as indicators of animal condition. No significant changes in proportional blubber weight or blubber thickness were seen over the last 40 years (1970–2009) for all age cohorts. This species appears to be well adapted to cope with changes in prey species availability, without impacting on body condition.     

Use of seaweeds for monitoring trace elements in coastal waters

Concentrations of a wide range of trace elements: arsenic, cadmium, cobalt, chromium, hafnium, nickel, thorium, uranium, zinc and the rare earth elements, cerium, europium, samarium, terbium and ytterbium were determined by instrumental neutron activation analysis in the brown alga,Fucus vesiculosus from Eckwarder Hörne, North Sea and from Rügen, Baltic Sea. Another brown alga,Sargassum filipendula from Sri Lanka, Indian ocean (representing an unpolluted control station) was similarly investigated. Cobalt, chromium and nickel concentrations were highest inF. vesiculosus from the North Sea while zinc was highest in samples from the Baltic Sea, reflecting high levels of these elements in coastal waters of the North and the Baltic sea. Cadmium, cobalt, nickel and zinc levels were lowest inS. filipendula from Sri Lanka, probably demonstrating lower levels of those elements in coastal waters. Concentration levels of hafnium, thorium, uranium, and the rare earth elements were highest inS. filipendula. Two years later in 1994,S. filipendula along withUlva sp. (green alga) was resampled from the same sampling site, and in addition to the above elements, six other trace elements (Ag, Ba, Br, Rb, Se and Sr) were determined.Sargassium filipendula showed a particular affinity for Ag, As, Br and Sr. For the other elements, marginal concentration differences were observed betweenS. filipendula andUlva sp., probably reflecting the regional background levels. Substantially higher concentrations of Hf, Th, U, and the rare earths were found again in the 1994Sargassum andUlva samples, reflecting the effect of a substrate rich in rare earth elements. The brown algae used in this study may be used to monitor trace elements in coastal waters.     

Use and abuse of Pb-isotope fingerprinting technique and GIS mapping data to assess lead in environmental studies

The danger to health, especially of young children, from lead-bearing particulates in the surface environment is still a valid concern. Given the multifaceted uses of lead today as well as historically, the sources of lead must be investigated to distinguish the etiology of lead contamination on the environment. The lead isotope finger printing technique based on isotopic ratio analyses (IRA) is one of the methods used commonly for provenancing lead sources in environmental studies throughout the world. This technique, however, has some inherited shortcomings. Therefore, caution must be used in the interpretation of the results, as this technique can lead to overestimations as well as underestimations of the true source etiology/apportionment of lead in specific environments. This paper illustrates this with some examples. Data on geographical information systems (GIS) mapping in urban regions are also presented in this paper.     

Using earth observation data from multiple sources to map rare habitats in a coastal conservation area

Kenfig NNR (National Nature Reserve) is a coastal sand dune system in south Wales, UK. The site is an important location for the conservation of the fen orchidLiparis loeselii, a significant proportion of the UK population is found solely on the site. Approaches to the mapping and monitoring of the habitats at Kenfig NNR using EO (Earth Observation) methods are investigated. Typical airborne EO missions over such sites produce more than a single source of EO data; these may include various optical imaging sensors with different spectral ranges, film cameras and ranging devices to measure topography. Conservation managers are thus presented with the problem of which sources of data to use when producing a land cover map of the site of interest. Using a data set gathered over the Kenfig NNR site, we investigate land cover mapping methods for conservation. The land cover types of interest typically cover small areas within a much larger site so they present a hard problem for the EO data and associated classification methods to solve. Land cover classifications produced from the data sets provide a set of competing hypotheses of land cover type for the site. Methods we use to resolve this competition between the data sets include voting methods, data fusion methods and a method utilising fuzzy logic to aggregate information. This paper is intended to act as an introduction to some of the issues involved in using EO data for habitat mapping in highly heterogeneous coastal dune environments and to present some preliminary results of the performance of each method.     

Use of data on avian demographics and site persistence during overwintering to assess quality of restored riparian habitat

Monitoring responses by birds to restoration of riparian vegetation is relatively cost-effective, but in most assessments species-specific abundances, not demography, are monitored. Data on birds collected during the nonbreeding season are particularly lacking. We captured birds in mist nets and resighted banded birds to estimate species richness and diversity, abundance, demographic indexes, and site-level persistence of permanent-resident and overwintering migrants in remnant and restored riparian sites in California. Species richness in riparian remnants was significantly higher than in restored sites because abundances of uncommon permanent residents were greater in remnants. Species richness of overwintering migrants did not differ between remnants and restored sites. Responses among overwintering migrants (but not permanent residents) to remnant and restored riparian sites differed. Capture rates were higher in remnant or restored riparian sites for 7 of 10 overwintering migratory species. For Lincoln's Sparrows (Melospiza lincolnii) and White-crowned Sparrows (Zonotrichia leucophrys) proportions of older birds were significantly higher in remnants, even though capture rates of these species were higher in restored sites. Overwinter persistence of 4 migrant species was significantly higher in remnant than in restored sites. A higher proportion of Hermit Thrushes (Catharus guttatus, 56.3%), older Fox Sparrows (Passerella iliaca, 57.1%), Lincoln's Sparrows (59.7%), and White-crowned Sparrows (67.8%) persisted in remnants than restored sites. Our results suggest restored riparian sites provide habitat for a wide variety of species in comparable abundances and diversity as occurs in remnant riparian sites. Our demographic and persistence data showed that remnants supported some species and age classes to a greater extent than restored sites.     

Life cycle, seasonal vertical distribution and feeding of Calanus finmarchicus in Skagerrak coastal water

 Day/night zooplankton sampling in Kosterfjorden, Sweden, gave information on population succession, vertical distribution and feeding of Calanus finmarchicus over 17 months. Copepodid Stage 1 and 2 (C-I, C-II) were present from December to August, indicating reproduction during most of the year. Mating and breeding for the overwintering generation mainly took place in February/March, resulting in peak abundance of C-I in March/April. Secondary breeding periods were in April and July/August, but the resulting recruitment from these were low. The relative recruitment success in the first spring was around 13 times higher than during the second spring. Low temperature in the surface water early in the year and depression of the spring phytoplankton bloom may have caused the failure in the second year. Population biomass peaked at >15 g dry weight m⁻² during the numeric peak of the youngest stages in March/April. A more sustainable level of high biomass of 8 to 10 g dw m⁻² was gradually built up during summer, mainly due to a continuous accumulation of C-Vs. Adults and C-Vs comprised the overwintering population, with 7 to 14% and 85 to 93%, respectively, for the 2 years, but only C-Vs staying in the deep water were in a resting state. Adult males showed a strong diurnal vertical migration (DVM) of the usual type from spring to early autumn and a reversed DVM during the cold season. They were in a feeding state throughout the year. Adult females showed the usual type of DVM during summer to autumn, but commonly a reversed DVM during winter to spring. They were usually in a feeding state, with no pronounced differences between surface and deep water or between day and night. C-Vs aggregated in the deep water from October to March and performed DVM in April to June. They were commonly feeding in the deep water between March and June but showed no or very low feeding activity there from July to February. C-Vs in the surface water were commonly feeding and showed the highest proportion of feeding in autumn, when the population in the deep water was inactive. C-V constituted up to nearly 100% of the population biomass, and therefore must be of profound ecological importance. Defined by this dominant role, the population of C. finmarchicus can be characterised as having an active period of feeding, reproduction and development from February to July with a following 6 to 7 months of resting in the deep water, when development is arrested and no feeding occurs. Received: 1 October 1999 / Accepted: 27 April 2000     

Evaluation of tests to assess the quality of mine-contaminated soils

An acid metal-contaminated soil from the Aljustrel mining area (a pyrite mine located in SW Portugal in the Iberian Pyrite Belt) was subjected to chemical characterisation and total metal quantification (Cd, Cr, Cu, Ni, Pb and Zn). Water-soluble metals were determined and a sequential extraction procedure was used to investigate metal speciation. Two bioavailable metal fractions were determined: a mobile fraction and a mobilisable fraction. Soil ecotoxicity was studied using a battery of bioassays: plant growth test and seed germination with cress (Lepidium sativum L.), earthworm (Eisenia fetida) mortality, E. fetida avoidance behaviour, luminescent inhibition of Vibrio fischeri and Daphnia magna immobilisation. Although the total content of Cu, Zn and Pb in the soil was large (362, 245 and 1,250 mg/kg dry matter, respectively), these metals were mostly structurally bound (87% for Cu, 81% for Zn and 89% for Pb) and, therefore, scarcely bioavailable. Nonetheless, the D. magna immobilization test using soil leachate showed an EC₅₀ (48 h) of 36.3% (v/v), and the luminescent inhibition of V. fischeri presented an EC₂₀ (15 min) of 45.2% and an EC₂₀ (30 min) of 10.7% (v/v), suggesting a considerable toxic effect. In the direct exposure bioassays, E. fetida avoided the mine soil at the highest concentrations (50%, 75% and 100% v/v). At the same soil concentrations, cress showed negligible growth. The results suggest the need to use a battery of toxicity tests, in conjunction with chemical methods, in order to assess the quality of mine-contaminated soils correctly.     

GIS and remote sensing as tools for conducting geo-hazards risk assessment along Gulf of Aqaba coastal zone,Egypt

Satellite remote sensing data, in addition to Geographic Information Systems (GIS), offers an excellent alternative to conventional mapping techniques in monitoring and mapping of geo-hazards areas. One of the most sustainable development projects in Egypt has been accomplished in Sinai, especially along and around the Gulf of Aqaba and the Gulf of Suez. Variations along the coastal zone of the Gulf of Aqaba have been identified through the analysis of multi-temporal satellite images with the aid of GIS analysis. The study area is subject to rapid and increasing changes in land-use/land-cover that resulting from natural and human activities such as flash flooding, seismic activity, landslides, and tourist and urban activities. This is in addition to the construction projects of roads, ports, PowerStation stations, mineral exploration, beaches, and tourist villages resulting from major environmental impacts. The current study aims to use Remote Sensing and GIS tools to investigate, monitor, and assess geo-hazards through the building of a geographical database. Several techniques have been developed over the last decade mostly to study the geological and geomorphologic characteristics of the terrain; land-use and land-cover changes. These are based on satellite imagery and Digital Elevation Models (DEM) to determine the topographic features, and geo-hazards maps. It is concluded that integrated approaches to monitoring can successfully be used to assess the environmental impacts along the Gulf of Aqaba coastal zone.     

GIS assessment of coastal vulnerability to climate change and coastal adaption planning in Vietnam

Vietnam’s coastal zone provides a diverse range of natural resources and favourable conditions for social and economic development. However, its coastal ecosystems are highly vulnerable, due to several natural coastal hazards, over-exploitation and other human activities. In spite of diverse interventions, Vietnam’s coastal zone continues to experience significant damage from floods, erosion and typhoons. These hazards are being intensified by climate change and associated rising sea levels. This paper assesses the potential vulnerability of Vietnam’s coast to climate change and discusses possible adaptation policies and plan to reduce the impacts. GIS analysis was used for the assessment of coastal vulnerability. Related literature was reviewed to develop detailed understanding of coastal adaptation to climate change. Adaptation policies and plans were appraised to identify potential coastal adaptation policies and plans that could be adapted by Vietnam. It was identified that vulnerability of the coastal zone of Vietnam could not be attributed only to climatic factors, but also to the physical condition of the coastline. Much of Vietnam’s coastline, particularly, areas around the Red River delta and the Mekong River have elevations below 1 m. These coastlines are largely developed and serve as economic centres of the country, which makes the coast more vulnerable to climate change and the rising sea level. The paper concluded that a non-structural approach (coastal buffer zones, building houses on stilts, storm warning systems, growing of flood-resistant crops and elevated storm shelters with medicine and food storage) could be used by Vietnam to adapt her low-lying coastline around the two deltas to climate change as this strategy enables vulnerable areas to be occupied for longer before eventual retreat. However, for these policies to be successful, it should be planned, implemented well in advance, monitored and evaluated over time.     

Feasibility of using salivary biomarkers to assess human exposure to chlorpyrifos

The objective of this study was to determine the feasibility of using salivary biomarkers to assess chlorpyrifos exposure using data collected from laboratory controlled animal study, as well as from farmers in Thailand and Nicaragua who applied chlorpyrifos in the field. Time-matched saliva and arterial blood samples were collected from rats and adult agricultural workers, while spot saliva samples were collected from children. Specimen samples were analyzed for chlorpyrifos using a commercially available enzyme-linked immunosorbent assay. The results from both animal and farmer studies show that chlorpyrifos is excreted into saliva. Nevertheless, salivary excretion of chlorpyrifos seems to differ from other pesticides, as evidenced by the lack of correspondence of chlorpyrifos levels between saliva and plasma samples. The lower chlorpyrifos concentrations in saliva collected from rats, and from farmers and their children, may have resulted from the rapid hydrolysis of chlorpyrifos during the intracellular passive diffusion in the salivary gland. In conclusion, chlorpyrifos is excreted into saliva; however, the majority of chlorpyrifos that is excreted in saliva may have been metabolized due to base-dependent hydrolysis. Because of this finding, it was hypothesized that it would be ideal to measure its metabolite, 3,5,6-trichloropyridinol, in saliva as the biomarker for chlorpyrifos exposure.     

Application of a coastal model to simulate present and future inundation and aid coastal management

This paper describes the application of coastal hydro-informatic modelling (using the TELEMAC Modelling System) to address management issues arising from projected hydrodynamical and morphological changes within a shallow, sandy estuarine environment. The model incorporates the complex interaction of ocean, terrestrial and atmospheric processes. The case study of the Dyfi Estuary, on the west coast of Wales, is highlighted here. As sea levels have risen locally and are predicted to rise further, a National Nature Reserve (Borth Bog), which has been reclaimed from tidal waters by embankments, will be at increasing risk from flooding episodes due to overtopping of these embankments at high tide. Present and predicted future tidal-fluvial scenarios have been modelled in the Dyfi Estuary in order to estimate the potential for flooding. In addition, areas of greatest velocity change and potential for sediment erosion/accretion have been identified. A further process that has been investigated is how salt marsh migration is affected by sea-level rise. This case study exemplifies some fundamental and complex physical processes inherent to estuaries, and shows how different management options can be assessed, before their implementation, through a modelling approach.     

Use of isotopic analysis of vertebrae in reconstructing ontogenetic feeding ecology in white sharks

We conducted stable 13C and 15N analysis on white shark vertebrae and demonstrated that incremental analysis of isotopes along the radius of a vertebral centrum produces a chronological record of dietary information, allowing for reconstruction of an individual's trophic history. Isotopic data showed significant enrichments in 15N with increasing sampling distance from the centrum center, indicating a correlation between body size and trophic level. Additionally, isotopic values verified two distinct ontogenetic trophic shifts in the white shark: one following parturition, marking a dietary switch from yolk to fish; and one at a total length of >341 cm, representing a known diet shift from fish to marine mammals. Retrospective trophic-level reconstruction using vertebral tissue will have broad applications in future studies on the ecology of threatened, endangered, or extinct species to determine life-long feeding patterns, which would be impossible through other methods.