共查询到19条相似文献,搜索用时 140 毫秒
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微生物对石油烃类的降解机理 总被引:4,自引:1,他引:4
文章分析了生物降解需要的条件、环境因素对石油烃微生物降解的影响,石油烃类的有氧降解机理、有氧降解方式,石油烃类的厌氧降解机理、厌氧降解过程中某些无机含氧化合物作受氢体的递氢过程,石油烃类化合物微生物降解难易程度,提出了以后应进一步重视原油的生物降解应用于我国微生物采油的研究。 相似文献
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为探究化学氧化法与微生物法联合修复技术在石油污染土壤修复中应用的可行性,文章采用联合修复实验,以过硫酸钠/过氧化钙为氧化剂,氧化预处理后联合生物修复,研究了修复过程中土壤石油烃含量、pH值、微生物数量以及石油烃分子分布的变化规律,比较了联合修复技术与单一生物修复对石油烃污染土壤修复效果的影响。实验结果表明,在过硫酸钠投加量0.3 mmol/g,n(Na2S2O8):n(CaO2):n(FeSO4):n(柠檬酸)为5:5:1:1条件下,石油烃(C10~C40)降解率为24.41%,其中C10~C25组分石油烃的降解率为-6.82%,C26~C40组分石油烃降解率为31.34%,氧化预处理后土壤添加石油烃降解菌进行生物修复,经联合修复后土壤中石油烃降解率可达85.13%,比直接进行生物降解的土壤,生物降解率提高了39.66%。修复后土壤的pH值由9.3... 相似文献
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石油污染土壤原位生物修复的强化实验研究 总被引:2,自引:0,他引:2
为研究添加营养物质和高效降解石油微生物对油污土壤生物修复的作用,通过分层土柱的方法,连续监测了不同条件下不同土层的含水率、石油烃含量、细菌数量及脱氢酶活性。结果表明:添加营养物质同时接种高效微生物可使降解效果明显改善,降解率比在自然条件下提高近50%,而单纯添加营养物质不接种高效微生物可使降解率比在自然条件下提高约25%。降解初期,上层土壤降解效果较好,而到中后期,中下层降解效果好于上层。微生物数量和脱氢酶活性与石油降解率之间存在良好的相关性,脱氢酶活性比微生物数量更能反映修复过程中微生物的存活状态。添加营养物质和高效降解石油微生物对油污土壤原位生物修复具有强化作用。 相似文献
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以长安大学渭水校区未被污染的粉质壤土为研究对象,通过土壤灭菌、添加由石油污染土壤红三叶草(Trifolium Repens Linn)根际修复区分离筛选得到的4株以原油作为惟一碳源和能源的高效石油烃降解菌(动性杆菌、藤黄微球菌、蜡状芽孢杆菌和短小芽孢杆菌),调控反应温度与石油烃初始浓度,研究在土壤中添加优势石油烃降解菌后石油烃降解动力学及其影响因子。结果表明:优势石油烃降解菌对土壤中石油烃降解起主导作用,在40d内,在2 000mg/kg石油烃浓度下添加石油烃降解菌其石油烃降解率是灭菌条件下的2倍左右,土壤中石油烃降解菌降解量为36~271mg/kg,非灭菌处理半衰期时间短于灭菌处理;在设定的实验温度范围内,石油烃降解速率随着温度增加逐渐加快,在(38±1)℃时残留量最小为1 662mg/kg,半衰期最短;土壤中的石油烃在浓度为2 000mg/kg时降解最快,随着初始浓度的增加,石油烃降解速率呈递减趋势,半衰期逐渐增长。 相似文献
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为对比勿色杆属菌种(Achromobater sp)SLTHX114株和黄色假单胞菌(Pseudomonas flavescens)SLTHX214株对原油降解特征的差异性,在40℃恒温有氧实验室条件下,使用两株原油降解菌对塔里木油田原油进行了生物降解模拟实验,分析了生物降解气组分、生物降解原油的族组分及生物标志化合物变化特征。实验结果表明,革兰氏阴性SLTHX214株对原油降解能力强于革兰氏阳性SLTHX114株,SLTHX114株对原油中的饱和烃具有明显的降解作用,而SLTHX214株更倾向于降解原油中的芳烃。对土壤石油污染修复和菌种选择具有一定的指示意义。 相似文献
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A 105-d field experiment was conducted to determine the potential of the slow-release fertilizer, Osmocote (Scotts, Marysville, OH), to stimulate the indigenous microbial biodegradation of petroleum hydrocarbons in an oil-spiked beach sediment on an intertidal foreshore in Singapore. Triplicate microcosms containing 80 kg of weathered sediment, spiked with 5% (w/w) Arabian light crude oil and 1.2% (w/w) Osmocote pellets, were established, together with control microcosms minus Osmocote. Relative to the control, the presence of the Osmocote sustained a significantly higher level of nutrients (NH(4)(+)-N, NO(3)(-)-N, and PO(4)(3-)-P) in the sediment pore water over the duration of the experiment. The metabolic activity of the indigenous microbial biomass, as measured using an intracellular dehydrogenase enzyme assay, was also significantly enhanced over the duration of the experiment in amended sediments. The loss of total recoverable petroleum hydrocarbons (TRPH) and biodegradation of total n-alkanes (C(10)-C(33)), branched alkanes (pristane and phytane), as well as total target polycyclic aromatic hydrocarbons (PAHs) (two- to six-ring), in both the control and Osmocote-amended sediments, followed a first-order biodegradation model. The first-order loss rate of total recoverable petroleum hydrocarbons was 2.57 times greater than that of the control. The hopane-normalized rate constants for total n-alkane, branched alkane, and total target PAH biodegradation in the Osmocote-treated sediments were 3.95-, 5.50-, and 2.45-fold higher than the control, respectively. Overall, the presence of Osmocote was able to significantly enhance and accelerate the biodegradation of aliphatics and PAHs in oil-contaminated sediments under natural field conditions in an intertidal foreshore environment. 相似文献
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Biodegradation of polycyclic aromatic hydrocarbons in oil-contaminated beach sediments treated with nutrient amendments 总被引:1,自引:0,他引:1
Microbial biodegradation of polycyclic aromatic hydrocarbons (PAHs) during the process of bioremediation can be constrained by lack of nutrients, low bioavailability of the contaminants, or scarcity of PAH-biodegrading microorganisms. This study focused on addressing the limitation of nutrient availability for PAH biodegradation in oil-contaminated beach sediments. In our previous study, three nutrient sources including inorganic soluble nutrients, the slow-release fertilizer Osmocote (Os; Scotts, Marysville, OH) and Inipol EAP-22 (Ip; ATOFINA Chemicals, Philadelphia, PA), as well as their combinations, were applied to beach sediments contaminated with an Arabian light crude oil. Osmocote was the most effective nutrient source for aliphatic biodegradation. This study presents data on PAH biodegradation in the oil-spiked beach sediments amended with the three nutrients. Biodegradation of total target PAHs (two- to six-ring) in all treatments followed a first-order biodegradation model. The biodegradation rates of total target PAHs in the sediments treated with Os were significantly higher than those without. On Day 45, approximately 9.3% of total target PAHs remained in the sediments amended with Os alone, significantly lower than the 54.2 to 58.0% remaining in sediment treatments without Os. Amendment with Inipol or soluble nutrients alone, or in combination, did not stimulate biodegradation rates of PAHs with a ring number higher than 2. The slow-release fertilizer (Os) is therefore recommended as an effective nutrient amendment for intrinsic biodegradation of PAHs in oil-contaminated beach sediments. 相似文献
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Nutrient amendment to oil-contaminated beach sediments is a critical factor for the enhancement of indigenous microbial activity and biodegradation of petroleum hydrocarbons in the intertidal marine environment. In this study, we investigated the stimulatory effect of the slow-release fertilizers Osmocote (Os; Scotts, Marysville, OH) and Inipol EAP-22 (Ip; ATOFINA Chemicals, Philadelphia, PA) combined with inorganic nutrients on the bioremediation of oil-spiked beach sediments using an open irrigation system with artificial seawater over a 45-d period. Osmocote is comprised of a semipermeable membrane surrounding water-soluble inorganic N, P, and K. Inipol, which contains organic N and P, has been used for oil cleanup on beach substrate. Nutrient concentrations and microbial activity in sediments were monitored by analyzing sediment leachates and metabolic dehydrogenase activity of the microbial biomass, respectively. Loss of aliphatics (n-C12 to n-C33, pristane, and phytane) was significantly greater (total loss between 95 and 97%) in oil-spiked sediments treated with Os alone or in combination with other nutrient amendments, compared with an unamended oil-spiked control (26% loss) or sediments treated with the other nutrient amendments (28-65% loss). A combination of Os and soluble nutrients (SN) was favorable for the rapid metabolic stimulation of the indigenous microbial biomass, the sustained release of nutrients, and the enhanced biodegradation of petroleum hydrocarbons in leached, oil-contaminated sediments. 相似文献
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氯代芳烃及其衍生物是一类毒性强、难降解的持久性有机化合物。在我国江河水体中检出率高,其中许多是优先监测污染物。水中的难降解有机污染物,可能通过几种微生物的一系列共代谢作用得到大部分,甚至是彻底的降解。本文针对水环境中的氟代芳烃污染物,分析了共代谢的类型、发生原因及影响因素。对利用共代谢机制修复水中氯代芳烃的最新研究进展进行了综述,并对本领域的发展前景进行了探讨。 相似文献
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John W. Wang Henrique C. G. Do Nascimento Teh Fu Yen 《Resources, Conservation and Recycling》1989,2(4)
Biodegradation of nitrogen-enriched lignite by soil bacteria previously acclimated in hydrogen peroxide-acetic acid lignitic substrate was carried out to assess the effects of nitrogen functional groups on the biodegradation of lignite. Chlorination, oxidation plus nitration, and ammoniation were applied in an attempt to modify the chemical structure of lignite so as to obtain a lignitic substrate that is more compatible to the enzymatical system of ordinary soil bacteria than that of unmodified lignite. Incorporation of nitrogen containing functional groups into lignite structure achieved 4.71 wt%, a value five times higher than that usually found in unmodified (raw) lignite. Data collected through a 16 day incubation time, monitoring production as well as depletion of both nitrate and organic acids, concluded that biodegradation was expedited by the incorporation of nitrogen into the structure of lignite. Twenty-eight compounds were identified based on the results from GC/MS analysis of the products. 相似文献
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Rajiv K. Sinha Vinod Chandran Brijal K. Soni Upendra Patel Ashok Ghosh 《The Environmentalist》2012,32(4):445-452
Earthworms are justifying the beliefs of Great Russian scientist Dr. Anatoly Igonin who said they are????disinfecting, detoxifying, neutralizing, protective and productive??. Studies indicate that some species of earthworms can ??bio-accumulate, biodegrade or bio-transform?? any toxic chemicals including ??heavy metals??, ??organochlorine pesticide??, ??herbicides??, and the lipophilic organic micro-pollutants like ??polycyclic aromatic hydrocarbons?? (PAHs). Worm vermicasts, due to the presence of ??hydrophilic?? groups in the ??lignin contents?? and ??humus??, also provide wonderful sites for ??adsorption?? of heavy metals and chemical pollutants in wastewater. Vermifiltration of wastewater using waste-eater earthworms is a newly conceived novel technology with several economic and environmental advantages. The earthworm??s body and the their ??vermicast?? work as a ??biofilter?? removing BOD5 by over 90?%, COD by 60?C80?%, TDSS by 90?C95?%, and toxic chemicals and pathogens from wastewater. This was a pioneering work done on an extremely ??toxic wastewater?? from the petroleum industry. It contained mixture of ??aliphatic?? and ??aromatic?? volatile petroleum hydrocarbons (C 10?CC 36) and ??organochlorines?? originating from the cooling liquids, waste engine and gear oil, waste transmission and brake fluid, grease, spilled petrol, and diesel oil. The aliphatic fraction contained cycloalkanes as well as a complex mixture of saturated toxic hydrocarbons. The aromatic fraction mainly consisted of PAHs, which are more toxic and persistent than the aliphatic part. The chemicals of concern were the total petroleum hydrocarbons (TPH), dichloromethane (DCM), dichloroethane (DCE), and t-butyl methyl ether (tBME). The tBME compound has raised global concern recently due to its high mobility and persistence in the environment and possible carcinogenicity. About 1,000 earthworms (species Eisenia fetida) were released in the soil of vermifilter bed. They not only tolerated and survived in the toxic petroleum environment but also bio-filtered and bio-remediated the dark-brown petroleum wastewater with a pungent smell into pale-yellow and odorless water indicating disappearance of all toxic hydrocarbons. The hydrocarbons C 10?CC 14 were reduced by 99.9?%, the C 15?CC 28 by 99.8?%, and the C 29?CC 36 by 99.7?% by earthworms. 相似文献