Rates of energy and phosphorus metabolism in Littorina snails (gastropoda)

Energy metabolism and phosphorus excretion were studied in Littorina littorea and L. saxatilis snails from the White Sea. Some elemental components of the body were determined quantitatively, and the rates of oxygen consumption and phosphorus excretion were estimated. All parameters were calculated relative to dry body weight (mg). Daily expenditures for energy metabolism in Littorina spp. averaged 3.6 ± 1.1%, and those for phosphorus metabolism were approximately four times lower (about 1%), with the total phosphorus content averaging 0.8% dry body weight. The atomic ratio of consumed oxygen to excreted phosphorus for both species averaged O: P = 1164 ± 140.     

The European whitefish, Coregonus lavaretus (L.), under conditions of technogenic pollution in the Kostomuksha tailing pond

The population structure and biological parameters of the European whitefish, Coregonus lavaretus (L.), have been analyzed in a technogenically polluted water body. The biochemical state of the whitefish and the fauna and morphology of helminths parasitizing them have been assessed. It is shown that the species composition of the fish, plankton, benthos, and parasite faunas of this water body are depleted and the morphological parameters of common fish parasites are altered. It is noted that, in addition to adaptive rearrangements in biochemical metabolism, the whitefish from the transformed water body are characterized by a reduction in the level of protein synthesis in muscles and the rate of aerobic energy metabolism in the liver and muscles.     

Functional-ecological role of biological diversity in populations and communities

The functional role of differentiation with respect to local population density, expression of responses to the presence of other individuals, and body weight has been analyzed from an ecoenergetic standpoint with consideration of known mathematical laws. The results indicate that increased variation in these parameters (i.e., their higher diversity) improves the efficiency of energy flow through the animal population: the input of energy increases, while its expenditures decrease. The greatest effect is achieved when the population is divided into alternative modal groups.     

Normalized Brain Weight as a Characteristic of the Relationship between the Body and Brain Weights in Birds

The growth of many internal organs of vertebrates is isometrically related to the growth of the body. Therefore, calculation of the index of an organ makes it possible to eliminate the basic dependence of organ weight on body weight and to reveal the effects of second-order factors. The relationship between the brain and body weights is allometric in all vertebrates, from fishes to birds and mammals, including man. To make this relationship isometric, brain weight should be divided by the square root of body weight, rather than body weight proper. This parameter was previously named normalized brain weight (NBW). The advantage of using NBW instead of cerebral index has been demonstrated for 88 subarctic populations of different bird species.     

Energy metabolism and growth of Monoporeia affinis Lindstr. in the Northern Lake Krivoe (Karelia)

The physiological method for estimating production based on the relationship between growth and energy metabolism has been used for calculating model growth curves for the amphipod Monoporeia affinis and comparing them with the results of field studies. Calculations demonstrated that the physiological growth curves fit the results of field observations best under certain conditions which have zoological sense: the growth curve should reflect the actual maximum body size of an animal in the given population, the duration of the life cycle, and the time required for reaching the reproductive size.     

Factors influencing wing size and body weight variation in the western honeybee

The patterns of wing size and body weight variation have been studied in worker bees, queens, and drones of the western honeybee. The influences of physiological states and ecological situations on the weight of the body and its parts and on the filling of the digestive tract have been analyzed. The results show that the differences in body weight between worker bees, queens, and drones do not correspond to differences in wing size between them. It is noted that worker bees, with their relatively small but strongly varying body weight, have acquired relatively large wings in the course of species phylogeny, a process accompanied by functional and morphophysiological differentiation of the members of bee families.     

Body condition and reproductive characteristics of female water voles (Arvicola terrestris L.)

The relationship between long-term dynamics of reproductive parameters and body condition were studied in female water voles from a cycling population. Body condition was estimated from the deviation of body weight (excluding the weight of the uterus with embryos) from the theoretically expected weight calculated from the equation of body weight regression with respect to body length. As the index of body condition increases upon transition from decline to peak in the population cycle, the numbers of corpora lutea and live embryos also increase and the risk of resorption of the entire litter becomes lower.     

Perfluorinated compounds in seafood from coastal areas in China

Diet is an important source of perfluorinated compound (PFC) exposure and seafood is an important diet component for coastal populations. Therefore, it is necessary to monitor the concentrations of PFCs in seafood. In this study, we measured thirteen PFCs in 47 fatty fish and 45 shellfish samples collected from six coastal provinces in China (Liaoning, Shandong, Jiangsu, Zhejiang, Fujian, and Guangdong), using an ultra-performance liquid chromatography and tandem mass spectrometry (UPLC–MS/MS). Perfluorooctanesulfonate (PFOS) was the dominant PFC in fatty fish which accounted for 38% of total PFCs, whereas perfluoroctanoic acid (PFOA) was the predominant PFC in shellfish. Concentrations of PFOS were ranged from less than 1.4 to 1627 pg/g wet weight in fatty fish, with the highest concentration in red drum from Jiangsu. Concentrations of PFOA in shellfish ranged from less than 5.4 to 7543 pg/g wet weight, with the maximum concentration found in briny clam also from Jiangsu. Compared with other studies, the PFC levels in seafood collected from our studied areas are relatively low. The estimated dietary intakes (EDI, pg/kg body weight /day) of PFOA, PFOS and total PFCs for the reference man (63 kg body weight) were calculated by multiplying the mean concentrations (pg/g wet weight) of PFOA, PFOS and total PFCs in six coastal provinces with the daily consumption data (g/day) from the fourth Chinese total diet study carried out in 2007. The highest EDI of PFOS and PFOA were found to be 694 pg/kg body weight/day and 914 pg/kg body weight/day in Guangdong and Jiangsu, respectively. However, the highest EDI of total PFCs was found in Fujian at 2513 pg/kg body weight/day. The EDI from seafood is much lower than the tolerable daily intake (TDI) recommended by the European Food Safety Authority in 2008 indicating low health risk of PFC exposure via eating seafood among the coastal populations in China.     

Influence of Body Weight and Size on the Mode of Wintering in Hibernators of the Family Sciuridae in Northeastern Russia

Energy expenditures in the Siberian chipmunk (Tamias sibiricus jacutensis Ognev, 1935) and long-tailed ground squirrel Citellus undulates jacutensis Brandt, 1843 during hibernation were compared. Energy expenditures were assessed by analyzing changes in body weight and gas exchange rate. The animals were weighed either monthly throughout the winter (26 ground squirrels and 9 chipmunks) or continuously (four to six times a day) in five ground squirrels (395 h) and three chipmunks (696 h). At a constant ambient temperature, the body weight of animals in both species decreased almost linearly during the day. The decrease in the body weight of hibernating grounds squirrels and chipmunks over a single short hibernation cycle was also approximated by a linear function, irrespective of species, age, and sex.     

Comparison and analysis of different approaches for estimating the human exposure to phthalate esters

The human exposure estimates for dibutyl (DBP) and bis(2-ethylhexyl) phthalate (DEHP) made by two models EUSES and ACC-Human, and by an estimation approach which utilized measured concentrations in exposure media, were compared. The approach which utilized the latest monitoring data for important exposure media, yielded median daily intakes for adult humans for DBP and DEHP of 2.7 and 5.6 microg/kg body weight per day, respectively, which were in the same range as previous estimates based on back-calculation from urinary metabolites. EUSES estimated average daily intakes of DBP and DEHP for humans that were between 8 and 13 times lower. ACC-Human does not estimate average daily intakes, but ACC-Human-estimated human milk concentrations/fugacities were more than a thousand times lower than measured concentrations/fugacities in human milk. It was concluded that the two models underestimate human exposure to phthalate esters because they consider only a few key pathways that are known to be important for other, more persistent, hydrophobic organic compounds. Further, it was shown that there are differences between the two models on the methodology for estimating concentrations in exposure media such as vegetation, milk, beef and fish. ACC-Human uses a mechanistic approach for estimating transfer through aquatic and terrestrial food chains that are known to be important for human exposure to persistent, hydrophobic organics and can, unlike EUSES, account for food chain metabolism. It proved difficult, however, to obtain organism metabolism rates needed as model inputs to ACC-Human. If exposure estimates of phthalate esters are needed, it is recommended to use an estimation approach based on high quality monitoring data as presented here and/or back-calculate daily intake from concentrations of metabolites in human urine samples from the general population.     

The Structure of Energy Supply for Tissue Adaptation in Small Mammals Differing in Ecological Specialization

Multivariate factor analysis of the data on liver bioenergetics of mountain and forest voles has been performed. The specific features of tissue energy metabolism are best differentiated in the space of characters pertaining to the succinic acid oxidation system. The role of the dominant oxidation substrate in the structure of energy supply for the adaptation of tissue to a set of environmental factors in specialized and widespread species is discussed.     

Toxic effects of brominated flame retardants in man and in wildlife

Brominated flame retardants (BFRs) are ubiquitous industrial chemicals, and many of them are produced in large volumes. Due to this fact, several BFRs are found in quantifiable levels in wildlife, as well as in humans. However, we are still lacking information on the effects of BFR in wildlife and, especially, in man. This review summarises the biological effects of polybrominated diphenyl ethers (PBDEs), tetrabromobisphenol A (TBBPA) and derivates, hexabromocyclododecane (HBCD) and polybrominated biphenyls (PBBs), however excluding other aspects such as environmental levels. These BFR groups were selected because of a large volume production (PBDEs, TBBPA and derivates), and availability of some toxicity data in spite of much lower production volumes (HBCD and PBBs). In addition, the increase in levels of PBDEs in human (breast milk) and wildlife samples during later time made it especially interesting to include this BFR group. PBDES: The commercial PBDE products predominantly consist of so-called penta-, octa- and decabromodiphenyl ether products. Each product consists of a rather narrow range of congeners and is named after the dominating congener as regards the bromination pattern. Generally, the PentaBDEs seem to cause adverse effects at the comparably lowest dose, whereas much higher doses were needed for effects of the DecaBDEs. The critical effects of PentaBDEs are those on neurobehavioural development (from 0.6 mg/kg body weight) and, at somewhat higher dose, thyroid hormone levels in rats and mice, of OctaBDEs on fetal toxicity/teratogenicity in rats and rabbits (from 2 mg/kg body weight), and of DecaBDEs on thyroid, liver and kidney morphology in adult animals (from 80 mg/kg body weight). Carcinogenicity studies, only performed for DecaBDEs, show some effects at very high levels, and IARC (1990) evaluates DecaBDEs not classifiable as to its carcinogenicity to humans. TBBPA: The toxicity of TBBPA in the experimental in vivo studies is suggested to be low. In most reported studies, only doses in g/kg body weight were effective, but at least one study suggested renal effects at around 250 mg/kg body weight. Although difficult to include and interpret in a quantitative risk assessment, the in vitro effects on immunological and thyroid hormones, as well as binding to erythrocytes should be noted. Before a solid standpoint could be reached on TBBPA toxicity additional studies must be performed. This statement is even more valid regarding the TBBPA derivates, where there is an almost complete lack of toxicity data. HBCD: Also in the case of HBCD, relevant toxicity studies are lacking. Based on the present animal studies, a critical effect is seen in the liver and on thyroid hormones (LOAEL 100 mg/kg body weight/day). However, in a recent short paper behavioural effects in mice pups were observed already at 0.9 mg/kg body weight, and behavioural effects may be a sensitive endpoint for HBCD, as well as for other BFRs. PBBS: Due to the Michigan accident in 1973-1974, many toxicity studies on PBBs are available. The critical experimental effects are those on reproduction and carcinogenicity, and a NOAEL of 0.15 mg/kg body weight/day could be suggested based on the cancer effects. In man no unequivocal effects have been observed, although in some studies neurological and musculoskeletal symptoms were suggested. Based on the carcinogenic effects in animals, a human TDI of 0.15 microg/kg body weight has been presented.To conclude, the toxicity data are almost entirely based on experimental models. There are differences among the BFR groups, as well as within these groups, both regarding type of toxic effect and at what dose it appears. As BFRs will continue to appear both in industrial applications and, even if the production has ceased, in our environment, there is a continued need for effects studies on BFRs.     

The response of gypsy moth (<Emphasis Type="Italic">Lymantria dispar</Emphasis> L.) larvae infected with nuclear polyhedrosis virus to induced resistance in birch (<Emphasis Type="Italic">Betula pendula</Emphasis> Roth.)

The effects of birch resistance induced by its artificial defoliation on the development of gypsy moth larvae and their sensitivity to viral infection and on the state of the antioxidant and detoxification systems of the insect midgut were studied. The dynamics of larval body weight; larval mortality and its etiology; glutathione-S-transferase (GT), nonspecific esterase (NE), and catalase (CAT) activities; and the ratio between the concentrations of oxidized and reduced thiol-containing compounds (RSSR/RSH) were estimated. In larvae feeding on the leaves of a previously defoliated plant, body weight was decreased, NE was inhibited, and the RSSR/RSH ratio was increased.     

Integrating energy and land-use planning: socio-metabolic profiles along the rural–urban continuum in Catalonia (Spain)

Abandoning fossil fuels and increasingly relying on low-density, land-intensive renewable energy will increase demand for land, affecting current global and regional rural–urban relationships. Over the past two decades, rural–urban relationships all over the world have witnessed unprecedented changes that have rendered their boundaries blurred and have lead to the emergence of “new ruralities.” In this paper, we analyze the current profiles of electricity generation and consumption in relation to sociodemographic variables related to the use of time and land across the territory of Catalonia, Spain. Through a clustering procedure based on multivariate statistical analysis, we found that electricity consumption is related to functional specialization in the roles undertaken by different types of municipalities in the urban system. Municipality types have distinctive metabolic profiles in different sectors depending on their industrial, services or residential role. Villages’ metabolism is influenced by urban sprawl and industrial specialization, reflecting current “new ruralities.” Segregation between work activity and residence increases both overall electricity consumption and its rate (per hour) and density (per hectare) of dissipation. A sustainable spatial organization of societal activities without the use of fossil fuels or nuclear energy would require huge structural and sociodemographic changes to reduce energy demand and adapt it to regionally available renewable energy.     

Effects of moderate pollution on toxic and trace metal levels in calves from a polluted area of northern Spain

This study evaluated the contribution of anthropogenic pollution to toxic metal residues (Cd, Pb and As) in cattle in an industrialized area of Asturias (northern Spain), and investigated possible implications of toxic metal exposure for metabolism of essential metal elements (Cu, Zn, Fe, Mn). Samples of liver, kidney, muscle and blood from animals aged 9-12 months were obtained from an industrialized area (78 calves) and from a rural area (92 calves). Samples were acid-digested, and levels of metals determined by atomic absorption spectrophotometry. Cadmium and lead contents in the liver and kidney were moderately and significantly higher in calves from the industrialized area (Cd: liver 29.6, kidney 161; Pb: liver 38.1, kidney 38.3 microg/kg wet weight) than in calves from the rural area (Cd: liver 22.9, kidney 96.4; Pb: liver 20.7, kidney 15.9 microg/kg kg wet weight). Although these toxic metals were only moderately raised, our results suggest that they may nevertheless have interfered with trace element metabolism, as reported previously for severely polluted regions. Notably, copper levels in calves from the industrialized area were generally low, and nearly half of these animals showed tissue copper levels indicating risk of copper deficiency.     

Ecological and physiological characteristics of seasonal biology of the mongolian hamster, Allocricetulus curtatus allan 1940 (Cricetinae, Rodentia)

Analysis of the seasonal dynamics of body weight and steroid hormones (cortisol, progesterone, and testosterone), the pattern of hypothermia, and basic reproductive parameters has been performed in the Mongolian hamster (Allocricetulus curtatus), a poorly studied rodent species inhabiting areas with a sharply continental climate. The results show that torpor bouts take place in wintering animals. Changes in body weight during the autumn-winter season are insignificant. The pattern of fluctuations in the levels of sex steroids and cortisol is similar to that in dwarf hamsters of the genus Phodopus. Seasonal features in the dynamics of basal cortisol level differentiate A. curtatus from the closely related Eversmann’s hamster (A. eversmanni), which may be due to differences in the social structure of these species.     

生态工程中群落模型植物灭螺机理研究

研究了夹竹桃、枫杨和羊蹄灭螺活性较强的正丁醇提取物和水提物对钉螺酯酶同工酶（EST）、糖原（Gn）和总蛋白（TPr）的生理生化影响。同工酶电泳分析表明,EST是动物体内一种重要的解毒酶系统,且是钉螺对外界刺激反应最强烈的同工酶之一,EST在整个提取物作用阶段变化明显,可作为钉螺中毒的病理指标。提取物能够显著地降低钉螺体内的糖原含量,在整体情况下,影响糖代谢的因素错综复杂,进行机制分析较为困难,可能是提取物对钉螺肝脏、消化道或酶综合作用的结果。提取物能够在一定程度上降低钉螺体内的蛋白质含量,但减少量不及糖原的减少那样显著,这表明灭螺药物对钉螺的致死作用可能更多地与使钉螺组织细胞中的能量代谢异常有关,但其生化机制还需进一步研究。实验从微观领域探究其对钉螺的杀伤机理,为最终构建人工灭螺植物群落提供理论依据。     

A Method for Forecasting the Dates of Mass Calving and Some Characteristics of the Rut and Calving in the Kalmyk Population of Saiga (Saiga tatarica L.)

A method for forecasting the dates of calving in the saiga population on the basis of average fetal body weight is proposed. The method is based on the relationship between the number of days before calving and the average weight of fetuses, which obviates the necessity of determining fetal age and exact gestation period in this species. The average length of gestation, the dates of the rut in the years studied, and the age of females that have mated immediately upon the onset of the rut and before its cessation have been determined. It is shown that the duration of mass calving depends on the proportion of mature males in the population.     

The Toad-Headed Agama Phrynocephalus guttatus (Reptilia: Agamidae): The Correlates of Survival in a Translocated Population in the Southwestern Kalmyk Republic

Observations of a translocated population of toad-headed agamas (Phrynocephalus guttatus) in the south of the Kalmyk Republic (1998–2001) have shown that seasonal differences in animal survival are manifested only in juveniles and the survival rate of juveniles is higher than that of adult individuals. A significant correlation between survival rate and sex is also characteristic only of juveniles: survival rate is higher in females than in males. In adult males, survival positively correlates with the level of activity; in juvenile males, with body size. In adult females, survival inversely correlates with body size and weight.     

Perfluorooctane sulfonate: A review of human exposure,biomonitoring and the environmental forensics utility of its chirality and isomer distribution

Perfluorooctane sulfonate (PFOS) found extensive use for over 60 years up until its restriction in the early 2000s, culminating in its listing under the Stockholm Convention on Persistent Organic Pollutants (POPs) in 2009. Efforts to minimise human body burdens are hindered by uncertainty over their precise origins. While diet appears the principal source for the majority of western populations (with other pathways like dust ingestion, drinking water and inhalation also important contributors); the role played by exposure to PFOS-precursor compounds followed by in vivo metabolism to PFOS as the ultimate highly stable end-product is unclear. Such PFOS-precursor compounds include perfluorooctane sulfonamide derivates, e.g., perfluorooctane sulfonamides (FOSAs) and sulfonamidoethanols (FOSEs). Understanding the indirect contribution of such precursors to human body burdens of PFOS is important as a significant contribution from this pathway would render the margin of safety between the current exposure limits and estimates of external exposure to PFOS alone, narrower than hitherto appreciated. Estimates derived from mathematical modelling studies, put the contribution of so-called “precursor exposure” at between 10% and 40% of total PFOS body burdens. However, there are substantial uncertainties associated with such approaches. This paper reviews current understanding of human exposure to PFOS, with particular reference to recent research highlighting the potential of environmental forensics approaches based on the relative abundance and chiral signatures of branched chain PFOS isomers to provide definitive insights into the role played by “precursor exposure”.