Tillage, cropping systems, and nitrogen fertilizer source effects on soil carbon sequestration and fractions

Quantification of soil carbon (C) cycling as influenced by management practices is needed for C sequestration and soil quality improvement. We evaluated the 10-yr effects of tillage, cropping system, and N source on crop residue and soil C fractions at 0- to 20-cm depth in Decatur silt loam (clayey, kaolinitic, thermic, Typic Paleudults) in northern Alabama, USA. Treatments were incomplete factorial combinations of three tillage practices (no-till [NT], mulch till [MT], and conventional till [CT]), two cropping systems (cotton [Gossypium hirsutum L.]-cotton-corn [Zea mays L.] and rye [Secale cereale L.]/cotton-rye/cotton-corn), and two N fertilization sources and rates (0 and 100 kg N ha(-1) from NH(4)NO(3) and 100 and 200 kg N ha(-1) from poultry litter). Carbon fractions were soil organic C (SOC), particulate organic C (POC), microbial biomass C (MBC), and potential C mineralization (PCM). Crop residue varied among treatments and years and total residue from 1997 to 2005 was greater in rye/cotton-rye/cotton-corn than in cotton-cotton-corn and greater with NH(4)NO(3) than with poultry litter at 100 kg N ha(-1). The SOC content at 0 to 20 cm after 10 yr was greater with poultry litter than with NH(4)NO(3) in NT and CT, resulting in a C sequestration rate of 510 kg C ha(-1) yr(-1) with poultry litter compared with -120 to 147 kg C ha(-1) yr(-1) with NH(4)NO(3). Poultry litter also increased PCM and MBC compared with NH(4)NO(3). Cropping increased SOC, POC, and PCM compared with fallow in NT. Long-term poultry litter application or continuous cropping increased soil C storage and microbial biomass and activity compared with inorganic N fertilization or fallow, indicating that these management practices can sequester C, offset atmospheric CO(2) levels, and improve soil and environmental quality.     

Carbon supply and storage in tilled and nontilled soils as influenced by cover crops and nitrogen fertilization

Soil carbon (C) sequestration in tilled and nontilled areas can be influenced by crop management practices due to differences in plant C inputs and their rate of mineralization. We examined the influence of four cover crops {legume [hairy vetch (Vicia villosa Roth)], nonlegume [rye (Secale cereale L.)], biculture of legume and nonlegume (vetch and rye), and no cover crops (or winter weeds)} and three nitrogen (N) fertilization rates (0, 60 to 65, and 120 to 130 kg N ha(-1)) on C inputs from cover crops, cotton (Gossypium hirsutum L.), and sorghum [Sorghum bicolor (L.) Moench)], and soil organic carbon (SOC) at the 0- to 120-cm depth in tilled and nontilled areas. A field experiment was conducted on Dothan sandy loam (fine-loamy, siliceous, thermic Plinthic Paleudults) from 1999 to 2002 in central Georgia. Total C inputs to the soil from cover crops, cotton, and sorghum from 2000 to 2002 ranged from 6.8 to 22.8 Mg ha(-1). The SOC at 0 to 10 cm fluctuated with C input from October 1999 to November 2002 and was greater from cover crops than from weeds in no-tilled plots. In contrast, SOC values at 10 to 30 cm in no-tilled and at 0 to 60 cm in chisel-tilled plots were greater for biculture than for weeds. As a result, C at 0 to 30 cm was sequestered at rates of 267, 33, -133, and -967 kg C ha(-1) yr(-1) for biculture, rye, vetch, and weeds, respectively, in the no-tilled plot. In strip-tilled and chisel-tilled plots, SOC at 0 to 30 cm decreased at rates of 233 to 1233 kg C ha(-1) yr(-1). The SOC at 0 to 30 cm increased more in cover crops with 120 to 130 kg N ha(-1) yr(-1) than in weeds with 0 kg N ha(-1) yr(-1), regardless of tillage. In the subtropical humid region of the southeastern United States, cover crops and N fertilization can increase the amount of C input and storage in tilled and nontilled soils, and hairy vetch and rye biculture was more effective in sequestering C than monocultures or no cover crop.     

Soil water nitrate and ammonium dynamics under a sewage effluent irrigated eucalypt plantation

Managed forests and plantations are appropriate ecosystems for land-based treatment of effluent, but concerns remain regarding nutrient contamination of ground- and surface waters. Monthly NO3-N and NH4-N concentrations in soil water, accumulated soil N, and gross ammonification and nitrification rates were measured in the second year of a second rotation of an effluent irrigated Eucalyptus globulus plantation in southern Western Australia to investigate the separate and interactive effects of drip and sprinkler irrigation, effluent and water irrigation, irrigation rate, and harvest residues retention. Nitrate concentrations of soil water were greater under effluent irrigation than water irrigation but remained <15 mg L(-1) when irrigated at the normal rate (1.5-2.0 mm d(-1)), and there was little evidence of downward movement. In contrast, NH4-N concentrations of soil water at 30 and 100 cm were generally greater under effluent irrigation than water irrigation when irrigated at the normal rate because of direct effluent NH4-N input and indirect ammonification of soil organic N. Drip irrigation of effluent approximately doubled peak NO3-N and NH4-N concentrations in soil water. Harvest residue retention reduced concentrations of soil water NO3-N at 30 cm during active sprinkler irrigation, but after 1 yr of irrigation there was no significant difference in the amount of N stored in the soil system, although harvest residue retention did enhance the "nitrate flush" in the following spring. Gross mineralization rates without irrigation increased with harvest residue retention and further increased with water irrigation. Irrigation with effluent further increased gross nitrification to 3.1 mg N kg(-1) d(-1) when harvest residues were retained but had no effect on gross ammonification, which suggested the importance of heterotrophic nitrification. The downward movement of N under effluent irrigation was dominated by NH4-N rather than NO3-N. Improving the capacity of forest soils to store and transform N inputs through organic matter management must consider the dynamic equilibrium between N input, uptake, and immobilization according to soil C status, and the effect changing microbial processes and environmental conditions can have on this equilibrium.     

Soil microbial communities and enzyme activities under various poultry litter application rates

The potential excessive nutrient and/or microbial loading from mismanaged land application of organic fertilizers is forcing changes in animal waste management. Currently, it is not clear to what extent different rates of poultry litter impact soil microbial communities, which control nutrient availability, organic matter quality and quantity, and soil degradation potential. From 2002 to 2004, we investigated the microbial community and several enzyme activities in a Vertisol soil (fine, smectitic, thermic, Udic Haplustert) at 0 to 15 cm as affected by different rates of poultry litter application to pasture (0, 6.7, and 13.4 Mg ha(-1)) and cultivated sites (0, 4.5, 6.7, 9.0, 11.2, and 13.4 Mg ha(-1)) in Texas, USA. No differences in soil pH (average: 7.9), total N (pasture: 2.01-3.53, cultivated: 1.09-1.98 g kg(-1) soil) or organic C (pasture average: 25-26.7, cultivated average: 13.9-16.1 g kg(-1) soil) were observed following the first four years of litter application. Microbial biomass carbon (MBC) and nitrogen (MBN) increased at litter rates greater than 6.7 Mg ha(-1) (pasture: MBC = >863, MBN = >88 mg kg(-1) soil) compared to sites with no applied litter (MBC = 722, MBN = 69 mg kg(-1) soil). Enzyme activities of C (beta-glucosidase, alpha-galactosidase, beta-glucosaminidase) or N cycling (beta-glucosaminidase) were increased at litter rates greater than 6.7 Mg ha(-1). Enzyme activities of P (alkaline phosphatase) and S (arylsulfatase) mineralization showed the same response in pasture, but they were only increased at the highest (9.0, 11.2, and 13.4 Mg ha(-1)) litter application rates in cultivated sites. According to fatty acid methyl ester (FAME) analysis, the pasture soils experienced shifts to higher bacterial populations at litter rates of 6.7 Mg ha(-1), and shifts to higher fungal populations at the highest litter application rates in cultivated sites. While rates greater than 6.7 Mg ha(-1) provided rapid enhancement of the soil microbial populations and enzymatic activities, they result in P application in excess of crop needs. Thus, studies will continue to investigate whether litter application at rates below 6.7 Mg ha(-1), previously recommended to maintain water quality, will result in similar improved soil microbial and biochemical functioning with continued annual litter application.     

Tradeoffs between soil, water, and carbon -- a national scale analysis from New Zealand

The tradeoffs between the regulation of soil erosion, provision of fresh water, and climate regulation associated with new Pinus radiata forests in New Zealand are explored using national models. These three ecosystem services for which there is strong demand are monetised as commodities (avoided soil erosion is NZ $1 per tonne; water is NZ $1 per cubic metre; and sequestered carbon is assumed to be NZ?$73 per tonne). This permits their summation on a spatial basis to produce a national map of the net benefit of these ecosystem services. Net benefit is spatially variable depending primarily on the relative mix of forest growth rates and demand for irrigation water. New P.?radiata forests (once mature) generally reduce mass-movement erosion by an order of magnitude. This provides significant benefits for erosion control where there are high natural rates of erosion. Benefits are especially large in catchments where high sedimentation is increasing flood risk and degrading aquatic ecosystems. The generally high growth rates of P.?radiata in New Zealand (8.5 tonnesCha(-1)yr(-1) on average for existing forest) add significant environmental benefits of carbon sinks to climate regulation. However, the reduction of water yield associated with new forests (between 30% and 50%) can neutralise these benefits in catchments where there is demand for irrigation water, such as the eastern foothills of the Southern Alps and the tussock grasslands in the South Island.     

Decomposition and nitrogen mineralization from biosolids and other organic materials: relationship with initial chemistry

Biosolids are effective forest fertilizers. In order to facilitate their use it is important that one be able to predict the amount and rate of mineralization of nutrients, particularly nitrogen, and the relationship between substrate chemistry and N release. We examined the relationships between substrate quality and nitrogen release in a variety of organic materials. Rates of decomposition and net N mineralization from four biosolids, wheat straw, paper fines, and Douglas-fir [Pseudotsuga menziesii (Mirb.) Franco] needle litter were measured during 391-d incubations in a greenhouse, and at two field sites in wet coastal and dry interior forests. Decomposition rates were best predicted by a model incorporating the ratio of carbon to organic matter. The decomposition model extrapolated well to the field when site-specific correction factors were applied. There was a weak relationship between rates of decomposition and net N mineralization. Rates of net N mineralization were best predicted by a model incorporating the initial organic N concentration and the proportion of phenolic C determined from solid-state 13C nuclear magnetic resonance (NMR) spectroscopy. The mineralization model extrapolated less well to the field, but the effect of substrate chemistry was still apparent. Among the four biosolids there was a strong correlation between organic N concentration and indices or protein determined from 13C NMR, suggesting that these protein indices may be useful for predicting N mineralization from biosolids. There was some evidence that the protein content and N mineralization in biosolids may be predictable from the sewage treatment process employed.     

REELFOOT LAKE SEDIMENTATION RATES AND SOURCES1

ABSTRACT: The three basins of Reelfoot Lake, which is located in northwestern Tennessee, were investigated using the Cs-137 tracer technique to determine rates of sediment deposition and to estimate the time before the basins will fill with sediment. Blue Basin, the largest of the three basins with 2922 ha, had an average annual sedimentation rate of 0.9 cm/yr from 1984 to 1984. The basin will become too shallow for most boating and recreational activities in about 200 years. Buck Basin, the central basin with 774 ha, had an average annual sedimentation rate of 1.1 cm/yr and will become too shallow for most recreational uses in about 100 years. Upper Blue Basin, the most upstream and smallest basin with 439 ha, had an average annual sedimentation rate of 1.7 cm/yr and will become too shallow for most recreational uses in about 60 years. Two important sources of sediment to Reelfoot Lake are erosion from a large number of soybean fields and channelization of many of the streams that flow into the lake. Changes in land management that would reduce erosion could increase the time the lake would remain usable for recreational activities.     

Effect of roundup ultra on microbial activity and biomass from selected soils

Herbicides applied to soils potentially affect soil microbial activity. The quantity and frequency of Roundup Ultra [RU; N-(phosphonomethyl)glycine; Monsanto, St. Louis, MO] applications have escalated with the advent of Roundup-tolerant crops. The objective of this study was to determine the effect of Roundup Ultra on soil microbial biomass and activity across a range of soils varying in fertility. The isoproplyamine salt of glyphosate was applied in the form of RU at a rate of 234 mg active ingredient kg(-1) soil based on an assumed 2-mm glyphosate-soil interaction depth. Roundup Ultra significantly stimulated soil microbial activity as measured by C and N mineralization, as well as soil microbial biomass. Cumulative C mineralization as well as mineralization rate increased above background levels for all soils tested with addition of RU. There were strong linear relationships between C and N mineralized, as well as between soil microbial C and N (r2 = 0.96 and 0.95, respectively). The slopes of the relationships with RU addition approximated three. Since the isopropylamine salt of glyphosate has a C to N ratio of 3:1, the data strongly suggest that RU was the direct cause of the enhanced microbial activity. An increase in the C mineralization rate occurred the first day following RU addition and continued for 14 d. Roundup Ultra appeared to be rapidly degraded by soil microbes regardless of soil type or organic matter content, even at high application rates, without adversely affecting microbial activity.     

Surface water and groundwater nitrogen dynamics in a well drained riparian forest within a poorly drained agricultural landscape

The effectiveness of riparian zones in mitigating nutrient in ground and surface water depends on the climate, management, and hydrogeomorphology of a site. The purpose of this study was to determine the efficacy of a well drained, mixed-deciduous riparian forest to buffer a river from N originating from a poorly drained grass seed cropping system. The study site was adjacent to the Calapooia River in the Willamette Valley, Oregon. Water was found to move from the rapid drainage of swale surface water. During winter hydrological events, the riparian forest also received river water. Low nitrate (NO3-) concentrations (0.2-0.4 mg NO3- -NL(-1)) in the shallow groundwater of the cropping system were associated with low rates of mineralization and nitrification (33 kg N ha(-1) yr(-1)) and high grass seed crop uptake of N (155 kg N ha(-1) yr(-1)). The riparian forest soil had higher rates of mineralization (117 kg N ha(-1) yr(-1)) that produced quantities of soil N that were within the range of literature values for plant uptake, leading to relatively low concentrations of shallow groundwater NO3 (0.6-1.8 mg NO3- -NL(-1)). The swale that dissected the cropping system and riparian area was found to have the highest rates of denitrification and to contribute dissolved organic C to the river. Given the dynamic nature of the hydrology of the Calapooia River study site, data suggest that the riparian forest plays a role not only in reducing export of NO3- from the cropping system to the river but also in processing nutrients from river water.     

Patterns of short-term sedimentation in a freshwater created marsh

This study investigated different sedimentation measurement techniques and examined patterns of short-term sedimentation in two 1-ha replicate created freshwater marshes in central Ohio, USA. Short-term (one-year) sediment accumulation above feldspar, clay, glitter, and sand artificial marker horizons was compared at different water depths and distances from wetland inflow. A sediment budget was also constructed from turbidity and suspended sediment data for comparison with marker horizons. Glitter and sand marker horizons were the most successful for measuring sediment accumulation (81-100% marker recovery), while clay markers were completely unsuccessful. The sedimentation rate for both wetlands averaged 4.9 cm yr(-1) (36 kg m(-2) yr(-1)), and ranged from 1.82 to 9.23 cm yr(-1) (12.4 to 69.7 kg m(-2) yr(-1)). Sedimentation rates in deep, open water areas were significantly higher than in shallow, vegetated areas for both wetlands (t test, p < 0.05). However, observed sedimentation patterns may be attributed more to preferential flow through open water areas than to water depth or presence of vegetation. Contrary to the expected spatial distribution, sedimentation was highly variable within the wetlands, suggesting that bioturbation and turbulence may cause significant resuspension or that high hydrologic loads may distribute sediments throughout the basins. A sediment budget estimated sediment retention of approximately 740 g m(-2) yr(-1) per wetland (43% removal rate), yet gross sediment accumulation was 36,000 g m(-2) yr(-1) measured by marker horizons. These results suggest that erosive forces may have influenced sedimentation, but also may indicate problems with the sediment budget calculation methodology.     

Nitrous oxide emission and denitrification in chronically nitrate-loaded riparian buffer zones

Riparian buffer zones are known to reduce diffuse N pollution of streams by removing and modifying N from agricultural runoff. Denitrification, often identified as the key N removal process, is also considered as a major source of the greenhouse gas nitrous oxide (N2O). The risks of high N2O emissions during nitrate mitigation and the environmental controls of emissions have been examined in relatively few riparian zones and the interactions between controls and emissions are still poorly understood. Our objectives were to assess the rates of N2O emission from riparian buffer zones that receive large loads of nitrate, and to evaluate various factors that are purported to control N emissions. Denitrification, nitrification, and N2O emissions were measured seasonally in grassland and forested buffer zones along first-order streams in The Netherlands. Lateral nitrate loading rates were high, up to 470 g N m(-2) yr(-1). Nitrogen process rates were determined using flux chamber measurements and incubation experiments. Nitrous oxide emissions were found to be significantly higher in the forested (20 kg N ha(-1) yr(-1)) compared with the grassland buffer zone (2-4 kg N ha(-1) yr(-1)), whereas denitrification rates were not significantly different. Higher rates of N2O emissions in the forested buffer zone were associated with higher nitrate concentrations in the ground water. We conclude that N transformation by nitrate-loaded buffer zones results in a significant increase of greenhouse gas emission. Considerable N2O fluxes measured in this study indicate that Intergovernmental Panel on Climate Change methodologies for quantifying indirect N2O emissions have to distinguish between agricultural uplands and riparian buffer zones in landscapes receiving large N inputs.     

Future carbon balance of China's forests under climate change and increasing CO2

The possible response of the carbon (C) balance of China's forests to an increase in atmospheric CO(2) concentration and climate change was investigated through a series of simulations using the Integrated Terrestrial Ecosystem Carbon (InTEC) model, which explicitly represents the effects of climate, CO(2) concentration, and nitrogen deposition on future C sequestration by forests. Two climate change scenarios (CGCM2-A2 and -B2) were used to drive the model. Simulations showed that China's forests were a C sink in the 1990 s, averaging 189 Tg C yr(-1) (about 13% of the global total). This sink peaks around 2020 and then gradually declines to 33.5 Tg C yr(-1) during 2091-2100 without climate and CO(2) changes. Effects of pure climate change of CGCM2-A2 and -B2 without allowing CO(2) effects on C assimilation in plants might reduce the average net primary productivity (NPP) of China's forests by 29% and 18% during 2091-2100, respectively. Total soil C stocks might decrease by 16% and 11% during this period. China's forests might broadly act as C sources during 2091-2100, with values of about 50 g Cm(-2)yr(-1) under the moderate warming of CGCM2-B2 and 50-200 g Cm(-2)yr(-1) under the warmer scenario of CGCM2-A2. An increase in CO(2) might broadly increase future C sequestration of China's forests. However, this CO(2) fertilization effect might decline with time. The CO(2) fertilization effects on NPP by the end of this century are 349.6 and 241.7 Tg C yr(-1) under CGCM2-A2 and -B2 increase scenarios, respectively. These effects increase by 199.1 and 126.6 Tg C yr(-1) in the first 50 years, and thereafter, by 150.5 and 115.1 Tg C yr(-1) in the second 50 years under CGCM2-A2 and -B2 increase scenarios, respectively. Under a CO(2) increase without climate change, the majority of China's forests would be C sinks during 2091-2100, ranging from 0 to 100 g Cm(-2)yr(-1). The positive effect of CO(2) fertilization on NPP and net ecosystem productivity would be exceeded by the negative effect of climate change after 2050. Under the CGCM2-A2 climate scenario and with direct CO(2) effects, China's forests may be a small C source of 7.6 Tg C yr(-1) during 2091-2100. Most forests act as C sources of 0-40 g Cm(-2)yr(-1). Under the CGCM2-B2 climate scenario and with direct CO(2) effects, China's forests might be a small C sink of 10.5 Tg C yr(-1) during 2091-2100, with C sequestration of most forests ranging from 0 to 40 g Cm(-2)yr(-1). Stand age structure plays a more dominant role in determining future C sequestration than CO(2) and climate change. The prediction of future C sequestration of China's forests is very sensitive to the Q(10) value used to estimate maintenance respiration and to soil water availability and less sensitive to N deposition scenario. The results are not yet comprehensive, as no forest disturbance data were available or predicted after 2001. However, the results indicate a range of possible responses of the C balance of China's forests to various scenarios of increase in CO(2) and climate change. These results could be useful for assessing measures to mitigate climate change through reforestation.     

Soil organic carbon decomposition and carbon pools in temperate and sub-tropical forests in China

Decomposition of soil organic carbon (SOC) is a critical component of the global carbon cycle, and accurate estimates of SOC decomposition are important for forest carbon modeling and ultimately for decision making relative to carbon sequestration and mitigation of global climate change. We determined the major pools of SOC in four sites representing major forest types in China: temperate forests at Changbai Mountain (CBM) and Qilian Mountain (QLM), and sub-tropical forests at Yujiang (YJ) and Liping (LP) counties. A 90-day laboratory incubation was conducted to measure CO(2) evolution from forest soils from each site, and data from the incubation study were fitted to a three-pool first-order model that separated mineralizable soil organic carbon into active (C(a)), slow (C(s)) and resistant (C(r)) carbon pools. Results indicate that: (1) the rate of SOC decomposition in the sub-tropical zone was faster than that in the temperature zone, (2) The C(a) pool comprised approximately 1-3% of SOC with an average mean residence time (MRT) of 219 days. The C(s) pool comprised approximately 25-65% with an average MRT of 78 yr. The C(r) pool accounted for approximately 35-80% of SOC, (3) The YJ site in the sub-tropical zone had the greatest C(a) pool and the lowest MRT, while the QLM in the temperature zone had the greatest MRT for both the C(a) and C(s) pools. The results suggest a higher capacity for long-term C sequestration as SOC in temperature forests than in sub-tropical forests.     

Carbon sinks and sources in China's forests during 1901-2001

This paper reports the annual carbon (C) balance of China's forests during 1901-2001 estimated using the Integrated Terrestrial Ecosystem C-budget model (InTEC). Annual carbon source and sink distributions are simulated for the same period using various spatial datasets including land cover and leaf area index (LAI) obtained from remote sensing, soil texture, climate, forest age, and nitrogen deposition. During 1901-1949, China's forests were a source of 21.0+/-7.8 Tg C yr(-1) due to disturbances (human activities). Its size increased to 122.3+/-25.3 Tg C yr(-1) during 1950-1987 due to intensified human activities in the late 1950s, early 1960s, 1970s and early 1980s. The forests became large sinks of 176.7+/-44.8 Tg C yr(-1) during 1988-2001, owing to large-scale plantation and forest regrowth in previously disturbed areas as well as growth stimulation by nondisturbance factors such as climatic warming, atmospheric CO(2) fertilization, and N deposition. From 1901 to 2001, China's forests were a small carbon source of 3.32 Pg C, about 32.9+/-22.3 Tg C yr(-1). The overall C balance in biomass from InTEC generally agrees with previous results derived from forest inventories of China's forests. InTEC results also include C stock variation in soils and are therefore more comprehensive than previous results. The uncertainty in InTEC results is still large, but it can be reduced if a detailed forest age map becomes available.     

Free-air CO2 enrichment of sorghum: soil carbon and nitrogen dynamics

The positive impact of elevated atmospheric CO(2) concentration on crop biomass production suggests more carbon inputs to soil. Further study on the effect of elevated CO(2) on soil carbon and nitrogen dynamics is key to understanding the potential for long-term carbon storage in soil. Soil samples (0- to 5-, 5- to 10-, and 10- to 20-cm depths) were collected after 2 yr of grain sorghum [Sorghum bicolor (L.) Moench.] production under two atmospheric CO(2) levels: (370 [ambient] and 550 muL L(-1) [free-air CO(2) enrichment; FACE]) and two water treatments (ample water and limited water) on a Trix clay loam (fine, loamy, mixed [calcareous], hyperthermic Typic Torrifluvents) at Maricopa, AZ. In addition to assessing treatment effects on soil organic C and total N, potential C and N mineralization and C turnover were determined in a 60-d laboratory incubation study. After 2 yr of FACE, soil C and N were significantly increased at all soil depths. Water regime had no effect on these measures. Increased total N in the soil was associated with reduced N mineralization under FACE. Results indicated that potential C turnover was reduced under water deficit conditions at the top soil depth. Carbon turnover was not affected under FACE, implying that the observed increase in soil C with elevated CO(2) may be stable relative to ambient CO(2) conditions. Results suggest that, over the short-term, a small increase in soil C storage could occur under elevated atmospheric CO(2) conditions in sorghum production systems with differing water regimes.     

Denitrification from a swine lagoon overland flow treatment system at a pasture-riparian zone interface

In manure disposal systems, denitrification is a major pathway for N loss and to reduce N transport to surface and ground water. We measured denitrification and the changes in soil N pools in a liquid manure disposal system at the interface of a pasture and a riparian forest. Liquid swine manure was applied weekly at two rates (approximately 800 and 1600 kg N ha-1 yr-1) to triplicate plots of overland flow treatment systems with three different vegetation treatments. Denitrification (acetylene block technique on intact cores) and soil N pools were determined bimonthly for 3 yr. The higher rate of manure application had higher denitrification rates and higher soil nitrate. Depth 1 soil (0-6 cm) had higher denitrification, nitrate, and ammonium than depth 2 soil (6-12 cm). The vegetation treatment consisting of 20 m of grass and 10 m of forest had lower denitrification. Denitrification did not vary significantly with position in the plot (7, 14, 21, and 28 m downslope), but nitrate decreased in the downslope direction while ammonium increased downslope. Denitrification ranged from 4 to 12% of total N applied in the manure. Denitrification rates were similar to those from a nearby dairy manure irrigation site, but were generally a lower percent of N applied, especially at the high swine effluent rate. Denitrification rates for these soils range from 40 to 200 kg N ha-1 yr-1 for the top 12 cm of soil treated with typical liquid manure that is high in ammonium and low in nitrate.     

Denitrification at a long-term forested land treatment system in the Piedmont of Georgia

Spray irrigation of forested land can provide an effective system for nutrient removal and treatment of municipal wastewater. Evolution of N2 + N2O from denitrifying activity is an important renovation pathway for N applied to forested land treatment systems. Federal and state guidance documents for design of forested land treatment systems indicate the expected range for denitrification to be up to 25% of applied N, and most forest land treatment systems are designed using values from 15 to 20% of applied N. However, few measurements of denitrification following long-term wastewater applications at forested land treatment sites exist. In this study, soil N2 + N2O-N evolution was directly measured at four different landscape positions (hilltop, midslope, toe-slope, and riparian zone) in a forested land treatment facility in the Georgia Piedmont that has been operating for more than 13 yr. Denitrification rates within effluent-irrigated areas were significantly greater than rates in adjacent nonirrigated buffer zones. Rates of N2 + N2O-N evolved from soil in irrigated forests ranged from 5 to 10 kg ha(-1) yr(-1) N on the three upland landscape positions and averaged 38 kg ha(-1) yr(-1) N within the riparian zone. The relationship between measured riparian zone denitrification rates and soil physical and chemical properties was poor. The best relationship was with soil temperature, with an r2 of 0.18. Overall, on a landscape position weighted basis, only 2.4% of the wastewater-applied N was lost through denitrification.     

Denitrification potential in urban riparian zones

Denitrification, the anaerobic microbial conversion of nitrate (NO3-) to nitrogen (N) gases, is an important process contributing to the ability of riparian zones to function as "sinks" for NO3- in watersheds. There has been little analysis of riparian zones in urban watersheds despite concerns about high NO3- concentrations in many urban streams. Vegetation and soils in urban ecosystems are often highly disturbed, and few studies have examined microbial processes like denitrification in these ecosystems. In this study, we measured denitrification potential and a suite of related microbial parameters (microbial biomass carbon [C] and N content, potential net N mineralization and nitrification, soil inorganic N pools) in four rural and four urban riparian zones in the Baltimore, MD metropolitan area. Two of the riparian zones were forested and two had herbaceous vegetation in each land use context. There were few differences between urban and rural and herbaceous and forest riparian zones, but variability was much higher in urban than rural sites. There were strong positive relationships between soil moisture and organic matter content and denitrification potential. Given the importance of surface runoff in urban watersheds, the high denitrification potential of the surface soils that we observed suggests that if surface runoff can be channeled through areas with high denitrification potential (e.g., stormwater detention basins with wetland vegetation), these areas could function as important NO3- sinks in urban watersheds.     

Aluminum output fluxes from forest ecosystems in Europe: a regional assessment

Data from 89 forested catchments and plots across Europe were used to define empirical relationships between aluminum leaching and input fluxes of major ions, output fluxes of major ions, ecosystem parameters such as soil pH, and combinations of these. Forests that release dissolved Al to seepage or surface waters are located primarily in areas receiving the highest loading of acid rain, and the output flux of Al shows the highest correlations to the throughfall flux of inorganic nitrogen, the output fluxes of NO3-, H+, and SO4(2-), and the mineral soil pH. If the speciation of Al is taken to be Al3+ (an overestimate), Al is released in a nearly 1:1 molar charge ratio with the sum of NO3- and SO4(2-) in runoff or seepage water over a wide range of basepoor bedrock types and acid deposition across Europe. The empirical data point to a threshold range of N deposition of 80 to 150 mmolc N m(-2) yr(-1) and a (less clearly defined) range of S deposition of 100 to 200 mmolc SO4(-2) m(-2) yr(-1) above which Al released from forests exceeds 100 mmolc Al m(-2) yr(-1). Within this threshold range, the sites that release little or no dissolved Al are those that continue to assimilate input N and/or have high soil pH (>4.5).     

Chloride effects on nitrogen dynamics in forested and suburban stream debris dams

Organic debris dams (accumulations of organic material) can function as "hotspots" of nitrogen (N) processing in streams. Suburban streams are often characterized by high flows that prevent the accumulation of organic debris and by elevated concentrations of solutes, especially nitrate (NO(3)(-)) and chloride (Cl(-)). In this study we (1) studied the effects of urbanization on the extent and characteristics of debris dams in large and small streams and (2) evaluated the effects of NO(3)(-) and Cl(-) on rates of N cycle processes in these debris dams. In some suburban streams debris dams were small and rare, but in others factors that reduce the effects of high stream flows fostered the maintenance of debris dams. Ambient denitrification enzyme activity (DEA) in these suburban and forested streams was positively correlated with stream NO(3)(-) concentrations. In laboratory microcosms, DEA in debris dam material from a forested reference stream was increased by NO(3)(-) additions. Chloride additions constrained the response of DEA to NO(3)(-) additions in material from the forested stream, but had no effect on DEA in material from streams with a history of high Cl(-) levels. Chloride additions changed the sign of net N mineralization from negative (consumption of inorganic N) to positive in debris dam material from the forested reference stream, but had no effect on net mineralization in material from streams with a history of exposure to Cl(-). Understanding the factors regulating the maintenance and N cycling activity of organic debris, and incorporating them into urban stream management plans could have important effects on N dynamics in suburban watersheds.