长江上游高山森林林窗对凋落物分解过程中可溶性碳的影响

采用凋落物分解袋法, 以川西高山森林典型乔木(四川红杉Larix mastersiana、岷江冷杉Abies faxoniana、红桦Betula albo-sinensis和方枝柏Sabina saltuaria)和灌木(高山杜鹃Rhododendron lapponicum和康定柳Salix paraplesia)凋落物为研究对象, 研究了林窗中心到郁闭林下不同生境(林窗中心、林冠林窗、扩展林窗和郁闭林下)中凋落物第一年分解不同关键时期(雪被形成期、雪被覆盖期、雪被融化期和生长季节)可溶性碳的动态特征。结果表明:6种凋落物可溶性碳和可溶性有机碳在冬季含量增加而在生长季节含量降低, 而可溶性无机碳含量在全年呈降低趋势;雪被形成期, 6种凋落物可溶性碳和可溶性有机碳在郁闭林下达到最大值, 而雪被覆盖期在林窗中心和林冠林窗达到最大值, 生长季节6种凋落物可溶性碳含量均低于初始含量。这表明全球气候变暖情景下, 冬季林内雪被覆盖时间和厚度降低且生长季节延长将减少凋落物可溶性碳含量, 且变化程度受到凋落物质量控制。     

岷江上游高山森林凋落叶在冬季河流中的质量损失特征

凋落叶在高山森林河流中的分解不仅是生态系统物质循环的重要内容,而且与森林养分流失以及下游水体环境密切相关,并可能受到冬季雪被变化和土壤季节性冻融的影响,但一直缺乏必要的关注。因此,以岷江上游高山森林4种代表性植物康定柳(Salix paraplesia)、高山杜鹃(Rhododendron lapponicum)、方枝柏(Sabina saltuaria)和四川红杉(Larix mastersiana)凋落叶为研究对象,采用凋落叶分解袋法,研究了一个冬季不同冻融时期(冻结初期、冻结期和融化期)的质量损失特征。经过一个冬季(162d)的分解,康定柳、高山杜鹃、方枝柏和四川红杉凋落叶分别完成了初始干重45.5%、18.9%、26.4%和23.8%的分解;除康定柳凋落叶质量损失在融化期最大外,其余3种凋落叶均表现为冻结初期最大;康定柳、方枝柏和四川红杉凋落叶质量损失与河流水环境的平均温度和正积温均表现出显著或极显著的正相关关系,且与河流流速和硝态氮显著正相关,而与河流pH显著负相关;高山杜鹃凋落叶质量损失除与HCO3-含量显著相关外,与河流水温及其它水质特征均无显著相关关系。这些结果表明高山森林河流流速及水环境特征显著影响了凋落叶分解及其相关的物质循环过程,但影响程度受到凋落叶特性的调控。     

川西亚高山云杉叶凋落物质量损失过程及土壤生物的作用

采用分解袋法,研究了川西亚高山云杉纯林与混交林叶凋落物的质量损失过程及土壤动物与微生物的作用。结果如下:(1)云杉叶凋落物分解过程中,土壤动物数量总体呈下降趋势,即土壤动物主要在分解前期发挥作用,其中,大型土壤动物早期以植食性的蛴螬为主,中期以捕食性的石蜈蚣为主,后期以腐食性的马陆为主;中小型土壤动物始终以螨类、跳虫和线虫为优势类群;相关分析表明线虫数量与云杉叶凋落物分解速率相关性最大,大型土壤动物次之。(2)云杉叶凋落物分解过程中,微生物数量总体呈上升趋势,即土壤微生物主要在分解后期发挥作用,进一步相关分析表明真菌数量与云杉叶凋落物分解速率相关性大于细菌和放线菌。(3)云杉纯林叶凋落物分解速率低于混交林,其中分解3、15、21、24个月时,纯林小孔径分解袋失重率分别为混交林的65.51%、95.73%、98.91%、94.8%,大孔径分解袋失重率分别为混交林的89.83%、92.95%、98.13%、97.13%,即,两类林分小孔径分解袋及凋落物分解前期失重率差异更为明显,表明凋落物分解速率既受底物质量的影响,也和参与分解的土壤生物有关,其中与线虫、大型土壤动物及真菌的数量相关性最大。     

Effects of acute γ-irradiation on community structure of the aquatic microbial microcosm

To characterise indirect effects of ionising radiation on aquatic microbial communities, effects of acute γ-irradiation were investigated in a microcosm consisting of populations of green algae (Chlorella sp. and Scenedesmus sp.) and a blue-green alga (Tolypothrix sp.) as producer; a ciliate protozoan (Cyclidium glaucoma), rotifers (Lecane sp. and Philodina sp.) and an oligochaete (Aeolosoma hemprichi) as consumer; and more than four species of bacteria as decomposers. Population changes in the constituent organisms were observed over 160 days after irradiation. Prokaryotic community structure was also examined by denaturing gradient gel electrophoresis (DGGE) of 16S rDNA. Principle response curve analysis revealed that the populations of the microcosm as a whole were not significantly affected at 100 Gy while they were adversely affected at 500-5000 Gy in a dose-dependent manner. However, some effects on each population, including each bacterial population detected by DGGE, did not depend on radiation doses, and some populations in the irradiated microcosm were larger than those of the control. These unexpected results are regarded as indirect effects through interspecies interactions, and possible mechanisms are proposed originating from population changes in other organisms co-existing in the microcosm. For example, some indirect effects on consumers and decomposers likely arose from interspecies competition within each trophic level. It is also likely that prey-predator relationships between producers and consumers caused some indirect effects on producers.     

1980~2009年长江下游地区油菜冻害时空特征研究

利用1980~2009年长江下游地区的气象数据和灾害资料,首先对油菜越冬期最低温度 ＜-5℃ 天数和蕾花期最低气温＜0℃天数进行了时空特征分析;然后对比了不同年代油菜关键生育期低温冻害的变化特点。结果表明:1980年以来,越冬期最低温度 ＜-5℃ 的天数和蕾花期最低气温＜0℃的天数均呈下降趋势;低温天数较大的地区是安徽和江苏省,低温天数较大的时期是1980s,1980~2009年油菜越冬期和蕾花期冻害出现的次数均有所减少,出现越冬期冻害的年份较少,发生蕾花期冻害的年份较多。安徽与江苏省是出现越冬期冻害的主要地区;除上海以外,其他省份均有半数以上的年份发生蕾花期冻害。长江下游地区可以采取育种、栽培和耕作等措施缓解关键生育期低温冻害对油菜产量的影响     

大冶湖浮游细菌群落结构及其环境影响因子研究

为了解大冶湖浮游细菌的群落结构,探究其环境影响因素,于2018年2月和2018年7月,采用16S rRNA高通量测序技术分析比较了大冶湖水体中枯水期和丰水期细菌群落结构及其多样性的差异,并通过冗余分析(RDA)探讨水体环境因子与细菌群落结构及其多样性的关系。结果表明:大冶湖的浮游细菌具有较高的菌群多样性(枯水期和丰水期的Shannon指数分别为3.856±0.642和4.165±0.608)。大冶湖水体的主要细菌门为变形菌门(Proteobacteria)、放线菌门(Actinobacteria)、蓝细菌门(Cyanobacteria)、拟杆菌门(Bacteroidetes)、厚壁菌门(Firmicutes)、浮霉菌门(Planctomycetes)、疣微菌门(Verrucomicrobia)、绿菌门(Chlorobi)、绿弯菌门(Chloroflexi)。其中,枯水期优势菌群为β-变形菌纲(Betaproteobacteria,44.73%),丰水期优势菌群为蓝细菌门(Cyanobacteria,31.39%)和放线菌门(Actinobacteria,31.37%)。冗余分析结果表明,硝酸盐(NO~-_3-N)、温度(T)和总氮(TN)为影响细菌群落分布的主要环境因子。     

Dynamics of leaf litter and soil respiration in a complex multistrata agroforestry system,Pernambuco, Brazil

High litter inputs in agroforestry systems contribute to soil microbial activity, soil fertility and productivity. Considering that the cycling of organic matter is essential to the maintenance of physical–chemical and microbiological properties of the soil, the aims of this work were to estimate the production, accumulation and decomposition of litter, and assess soil microbial respiration in a complex multistrata agroforestry system located in the north-east of Brazil. This agroforestry system has three strata formed by forest and fruit trees and species of multiple uses. During 3 years (2011–2013), leaf litter was sampled monthly to account for litterfall and quarterly to account for litter accumulation. The rates of litter decomposition were estimated using the ratio produced-to-accumulated litter, and the correlation between litter fall and rainfall was calculated. Precipitation data were provided by the water and climate agency of Pernambuco (APAC). Soil samples (0–15 cm) were also taken quarterly, simultaneously with the litter accumulation samples, and soil microbial respiration was assessed using the capture, by a KOH solution, of the evolved CO₂. The annual production of leaf litter was stable in the 3 years of study in this agroforestry system, and the monthly input of litter to the soil was influenced by rainfall, being higher in the dry seasons. The accumulated litter on the ground was constant, as was microbial activity (respiration) through time. The estimated litter decomposition rates were 1.49 (first year), 1.33 (second year) and 1.42 (third year), being considered rapid rates of decomposition. This guarantees (to the farmer) that this system is capable of maintaining soil fertility and eliminates the need for chemical fertilizers.     

上海外环绿地闵行段不同植被类型下土壤有害动物的群落结构

为探讨上海闵行段外环绿地不同植被类型下土壤有害动物的群落组成、群落多样性及其与理化因子的关系,于2008年11月至2009年7月,对上海闵行段外环绿地6个样地的土壤动物群落按凋落物层和0～15 cm土层按季节进行调查研究。共获得土壤有害动物共359只,隶属于昆虫纲8目23科。其中凋落物层共获得土壤动物242只,隶属7目19科,优势类群为蚁科、管蓟马科和蚧科幼虫,真土层共获得土壤有害动物117只,隶属于4目8科,优势类群为蚁科。相对于常绿阔叶林和落叶针叶林,落叶阔叶林表现出较高的类群数、密度以及群落多样性。各样地pH和总氮均存在显著差异,土壤有害动物主要类群的密度多与总氮和总磷含量相关（〖WTBX〗p〖WTBZ〗<005）。〖     

镇江内江湿地植物群落演替动态研究

采用“空间代替时间”方法,于镇江内江湿地选择4类典型样地,分别代表群落演替的4个不同时间段,从物种组成、多样性及生活型等方面研究湿地植被的演替规律,从而探索受损群落的修复途径。结果表明：不同演替阶段植物群落的物种组成、多样性指数和生活型变化明显。物种丰富度指数和多样性指数随演替呈上升趋势,且演替中后期最高,均匀度随演替递增,而优势度递减;在生活型上,多年生草本的重要值随演替先降后升,其物种数逐渐上升并在后期维持相对稳定,并在各演替阶段处于主导地位。而一、二年生草本植物的重要值和物种数均为先升后降;在科属总数上,科属数随演替逐渐增加,其中,禾本科植物始终处于优势种地位。     

Carbon Emission by Soils in Forests Damaged by the Siberian Moth

In the southern-taiga low grass–green moss fir forest completely defoliated by the Siberian moth, the rate of soil respiration in the third year after the pest population outbreak was 1.5 times higher than in an undisturbed tree stand. This was explained by a significant increase in the abundance and activity of soil microorganisms in the pest-defoliated forest, which occurred due to the qualitative changes in the litter composition and the increased temperature and moisture in the upper soil layers. The rate of carbon emission from the soil in the defoliated forest was 41.7 kg/ha per day, compared to 28.3 kg/ha per day in the undisturbed fir forest.     

Investigating freshwater periphyton community response to uranium with phospholipid fatty acid and denaturing gradient gel electrophoresis analyses

Periphyton communities can be used as monitors of ecosystem health and as indicators of contamination in lotic systems. Measures of biomass, community structure, and genetic diversity were used to investigate impacts of uranium (U) exposure on periphyton. Laboratory exposures of periphyton in river water amended with 238U were performed for 5 days, followed by 2 days of U depuration in unamended river water. Productivity as measured by biomass was not affected by concentrations up to 100 microg238U L(-1). Phospholipid fatty acid (PLFA) profiles and denaturing gradient gel electrophoresis (DGGE) banding patterns revealed no changes in community or genetic structure related to U exposure. We suggest that the periphyton community as a whole was not significantly impacted by exposures of 238U up to a concentration of 100 microgL(-1). These findings have significance for the assessment and prediction of U impacts on aquatic ecosystems.     

Long-term field application of sewage sludge increases the abundance of antibiotic resistance genes in soil

Sewage sludge and manure are common soil amendments in crop production; however, their impact on the abundance and diversity of the antibiotic resistome in soil remains elusive. In this study, by using high-throughput sequencing and high-throughput quantitative PCR, the patterns of bacterial community and antibiotic resistance genes (ARGs) in a long-term field experiment were investigated to gain insights into these impacts. A total of 130 unique ARGs and 5 mobile genetic elements (MGEs) were detected and the long-term application of sewage sludge and chicken manure significantly increased the abundance and diversity of ARGs in the soil. Genes conferring resistance to beta-lactams, tetracyclines, and multiple drugs were dominant in the samples. Sewage sludge or chicken manure applications caused significant enrichment of 108 unique ARGs and MGEs with a maximum enrichment of up to 3845 folds for mexF. The enrichment of MGEs suggested that the application of sewage sludge or manure may accelerate the dissemination of ARGs in soil through horizontal gene transfer (HGT). Based on the co-occurrence pattern of ARGs subtypes revealed by network analysis, aacC, oprD and mphA-02, were proposed to be potential indicators for quantitative estimation of the co-occurring ARGs subtypes abundance by power functions. The application of sewage sludge and manure resulted in significant increase of bacterial diversity in soil, Proteobacteria, Acidobacteria, Actinobacteria and Chloroflexi were the dominant phyla (> 10% in each sample). Five bacterial phyla (Chloroflexi, Planctomycetes, Firmicutes, Gemmatimonadetes and Bacteroidetes) were found to be significantly correlated with the ARGs in soil. Mantel test and variation partitioning analysis (VPA) suggested that bacterial community shifts, rather than MGEs, is the major driver shaping the antibiotic resistome. Additionally, the co-occurrence pattern between ARGs and microbial taxa revealed by network analysis indicated that four bacterial families might be potential hosts of ARGs. These results may shed light on the mechanism underlining the effects of amendments of sewage sludge or manure on the occurrence and dissemination of ARGs in soil.     

Comparison of understorey bird species in relation to edge–interior gradient in an isolated tropical rainforest of Malaysia

Forest fragmentation results in a loss of forest interior and an increase in edge habitat. As a result, these changes may affect the bird species distribution and composition. We studied how understorey bird community composition and habitat variables changed along an edge-to-interior gradient in a fragmented lowland rainforest in Peninsular Malaysia. Birds and environmental variables were recorded at each of the 93 sampling points distributed along the 14 parallel transects that were systematically placed across the gradient of distance from the forest edge to the forest interior. Species composition was different along the edge–interior gradient, although only a few species were strictly confined to either edge or interior habitat. Based on bird–habitat associations along the edge–interior gradient, some of these edge-preferred species occurred in high numbers at the matrix surrounding the patch. In contrast, the interior-specialist group, mainly terrestrial insectivores, avoided the forest edge and was positively associated with humidity, canopy cover, the number of dead trees, percentage of litter cover, and depth of the litter layer. Species endemic to the Sunda subregion were more abundant in the interior of the forest. From a conservation perspective, forest remnants in the lowlands of Peninsular Malaysia that have a deep leaf litter layer, dense canopy cover, high number of dead trees, and high relative humidity are able to support understorey bird species that are sensitive to edge effects. The forest has important conservation value even though it is fragmented and isolated.     

Distribution of Fine Roots of Coniferous Trees over the Soil Profile under Conditions of Pollution by Emissions from a Copper-Smelting Plant

The distribution of fine conducting roots of Siberian spruce, Siberian fir, and Scotch pine over the soil profile was studied in the taiga ecosystems polluted with heavy metals and SO₂. Under conditions of heavy pollution, tree roots were not found in the forest litter. Regardless of the level of technogenic load, the largest amount of conducting roots concentrated in the upper layer of mineral soil.     

Carabids in a Spring Wheat Agrocenosis to the South of Sverdlovsk Oblast and the Effect of Insecticide Treatment on Their Populations

Species composition, community structure, and seasonal population dynamics of carabids in spring wheat fields were studied in the vicinity of Yekaterinburg. In addition, the effects of crop treatment with the pyrethroid insecticide Decis on carabid populations was analyzed. The results showed that the numbers, species diversity, and the prevalence of certain trophic groups of carabids changed during the season. After the treatment of small areas with Decis, the number of carabids in catches decreased for a short period of time. During the second and third weeks after the treatment, the numbers of carabids increased above the control level. The structural rearrangement of the carabid community was observed in the areas treated with the insecticide.     

农户生计多样性发展阶段研究——基于脆弱性与适应性维度

为了观察农户生计活动复杂化过程,分析弹性在可持续生计中发挥的作用,推动扶贫开发战略从识别贫困人口向防御贫困转变,本文采用曲线拟合、核密度函数非参数估计算法等实证研究方法,利用陕西省安康农户生计与环境调查数据,基于脆弱性、适应性维度对农户生计多样性发展阶段进行了识别、划分,系统分析了弹性在农户、社区不同尺度上、阶段上对于农户生计脆弱性与适应性的作用过程。研究发现:生计多样性发展阶段随尺度有所不同,随着适应性提高,在社区尺度上,生计活动多样性指数先增加后减小,在农户尺度上,则是先减小后增加;农户生计活动存在高脆弱性、低适应性的尝试阶段,在经历了这个阶段之后,伴随着农户适应性的提高,脆弱性会同时增加;在社区尺度上,弹性作用下伴随着收入提高,脆弱性会逐步降低,在农户尺度上,弹性作用下伴随着收入提高,生计活动脆弱性会提高,形成"高风险、高收益"的情况。研究发现说明农户、社区存在着"多样性尝试"发展阶段,在一定尺度上保持适度脆弱性有利于社区、地域的可持续发展,作为联系不同尺度生计活动脆弱性、适应性的关键,农户在这个阶段完成生计多样性水平专业化过程,社区在这个阶段形成地区特色的生计活动。扶贫开发工作应把握好这一时机,提高工作措施效果和绩效。在扶贫开发工作实践中,在目标制定上不仅需要考虑收入因素,还需要考虑生计活动复杂性因素,政策措施充分利用"多样性尝试"发展时机,同时主动开展替代生计研究、落实工作,提高贫困防御能力,推动农户、社区可持续发展。     

武汉沉湖湿地水鸟群落特征及其与富营养化关系研究

富营养化是水中氮、磷等营养物质大量积累引起的水质污染现象,直接导致水体群落结构的改变,这一定程度上引起了以水生生物为食的水鸟的群落特征的变化。为找寻水鸟群落特征与富营养化两者的关系,2010年1月~2013年1月3年内按春夏秋冬4季对武汉沉湖湿地自然地保护区水鸟进行了调查,沉湖和张家大湖的水鸟多样性有明显的周年变化规律,多样性指数冬季最高,最高值分别为12.19和9.89;春夏季最低,最低值分别为7.53和2.45;对比分析了两个子湖,沉湖湖区与张家大湖湖区富营养化指数TLI分别为64和78,沉湖水鸟种类、数量和多样性指数皆高于张家大湖,湖泊富营养化会影响水鸟多样性。     

上海滩涂春季鸻形目鸟类群落及围垦后生境选择

上海市位于长江口，拥有大量可开发的滩涂资源，但是由于不合理的开发方式，滩涂景观和生物群落发生改变。在2004年春季，鸻形目鸟类迁徙中转停留时，实地调查位于上海东部沿岸的10个调查点，研究滩涂生境指标与鸻形目鸟类群落关系。此次观察到的鸻形目鸟类有13种，优势种有尖尾滨鹬和黑腹滨鹬，而20年前的类似调查则观察到23种，优势种为黑腹滨鹬、细嘴滨鹬和红颈滨鹬。通过分析滩涂结构、植被分布与鸻形目鸟类群落之间的关系发现鸻形目鸟类对生境选择中，滩涂结构完整度、藨草带和光滩带的宽度、周边景观的多样性、人为干扰程度都是影响鸻形目鸟类栖息地选择的关键因子。最后，分析了鸻形目鸟类群落与栖息地景观的关系，并就如何改善围垦堤内的生态环境提出一些建议。     

Monitoring of cadmium in "on" and "off" date palms

The study demonstrated that the mature dates we investigated were considered safe for human consumption. However, our findings revealed that much of the early fruit and leaves, which appeared at the advent of the fruiting season, possessed elevated levels of cadmium (Cd) that could accumulate in the food chain and thus impact adversely on the environment. In addition, animal feed for livestock contains date leaves, which should be restricted to the mature ones. The monitoring of cadmium in date palms is of considerable interest to environmental science and the thrust of this work, therefore, involved measurement, by ICP, of Cd in dates and corresponding leaf specimens, and evaluation of its distribution during the developmental stages of the fruiting season. Thirty-six date samples and 36 leaf specimens of the Fard cultivar were collected from "on" and "off" date palms during the Kimri, Bisir and Rutab stages of the fruiting season and subjected to suitable digestion procedures. Sample masses of typically 1 g (dry weight) were prepared in 25 ml dilute acid solution and investigated for trace levels of Cd by ICP-AES. Special attention was paid to contamination and the validation of our methodology. The Cd "threshold" in our study was 50 ng/g, in keeping with the levels of tolerance appearing in the literature. For the dates we found elevated levels of Cd [> 50 ng/g] in most of the samples, for both categories of "on" and "off" trees, during the Kimri stage. In the case of the leaves, the "on" samples revealed significant values up to 125 ng/g in Bisir, in some cases, but the trend for the "off" trees remained the same with the highest levels [> 100 ng/g] recorded during Kimri. Safe levels were attained during Rutab for all specimens. The possibility of a connection between Cd toxicity and the alternate-bearing phenomenon is discussed and this could be the subject of future interest.