Inbreeding Depression Accumulation across Life‐History Stages of the Endangered Takahe

Abstract: Studies evaluating the impact of inbreeding depression on population viability of threatened species tend to focus on the effects of inbreeding at a single life‐history stage (e.g., juvenile survival). We examined the effects of inbreeding across the full life‐history continuum, from survival up to adulthood, to subsequent reproductive success, and to the recruitment of second‐generation offspring, in wild Takahe ( Porphyrio hochstetteri ) by analyzing pedigree and fitness data collected over 21 breeding seasons. Although the effect size of inbreeding at individual life‐history stages was small, inbreeding depression accumulated across multiple life‐history stages and ultimately reduced long‐term fitness (i.e., successful recruitment of second‐generation offspring). The estimated total lethal equivalents (2B) summed across all life‐history stages were substantial (16.05, 95% CI 0.08–90.8) and equivalent to an 88% reduction in recruitment of second‐generation offspring for closely related pairs (e.g., sib–sib pairings) relative to unrelated pairs (according to the pedigree). A history of small population size in the Takahe could have contributed to partial purging of the genetic load and the low level of inbreeding depression detected at each single life‐history stage. Nevertheless, our results indicate that such “purged” populations can still exhibit substantial inbreeding depression, especially when small but negative fitness effects accumulate across the species’ life history. Because inbreeding depression can ultimately affect population viability of small, isolated populations, our results illustrate the importance of measuring the effects of inbreeding across the full life‐history continuum.     

Economics of Grassland Conversion to Cropland in the Prairie Pothole Region

Abstract: Much of the remaining grassland, particularly in North America, is privately owned, and its conversion to cultivated cropland is largely driven by economics. An understanding of why landowners convert grassland to cropland could facilitate more effective design of grassland‐conservation programs. We built an empirical model of land‐use change in the Prairie Pothole Region (north‐central United States) to estimate the probability of grassland conversion to alternative agricultural land uses, including cultivated crops. Conversion was largely driven by landscape characteristics and the economic returns of alternative uses. Our estimate of the probability of grassland conversion to cultivated crops (1.33% on average from 1979 to 1997) was higher than past estimates (0.4%). Our model also predicted that grassland‐conversion probabilities will increase if agricultural commodity prices continue to follow the trends observed from 2001 to 2006 (0.93% probability of grassland conversion to cultivated crops in 2006 to 1.5% in 2011). Thus, nearly 121 million ha (30 million acres) of grassland could be converted by 2011. Conversion probabilities, however, are spatially heterogeneous (range 0.2% to 3%), depending on characteristics of a parcel (e.g., soil quality and economic returns). Grassland parcels with relatively high‐quality land for agricultural production are more likely to be converted to cultivated crops than lower‐quality parcels and are more responsive to changes in the economic returns on alternative agricultural land uses (i.e., conversion probability increases by a larger magnitude for high‐quality parcels when economics returns to alternative uses increase). Our results suggest that grassland conservation programs could be proactively targeted toward high‐risk parcels by anticipating changes in economic returns, such as could occur if a new biofuel processing plant were to be built in an area.     

Prairie Landscape Change and Flooding in the Mississippi River Valley

Extensive landscape alteration of prairie in the U.S. from agricultural expansion has reduced waterfowl populations and increased precipitation runoff into regional river basins. Satellite imagery shows that prairie landscapes have been less altered in Canada than in the U.S. Long-term, broad-scale precipitation data indicate that in both countries precipitation has varied widely but has not increased over time. Nevertheless, flow rates of unregulated U.S. rivers have increased, but there have been no detectable changes in flow rates of Canadian rivers. Neither of two competing hypotheses advanced to explain increasing flood magnitudes—climate change and channel confinement—can account for these results. Thus, the increased magnitudes of floods in the Mississippi River Valley over the last several decades may be at least partially related to extensive changes in agricultural land use resulting in reduction of natural upland vegetation and wetland drainage in the upper reaches of this watershed.     

Effect of Family Support on the Success of Translocated Black-Tailed Prairie Dogs

Abstract:  Translocation has become a widely used conservation tool but remains only marginally successful. High mortality is often attributed to predation, but for highly social species, founder group composition may also play a critical role in postrelease survival. I compared the fitness of black-tailed prairie dogs translocated with or without their family groups. Animals in the family translocated groups were individually marked and observed until coterie membership was determined. Nonfamily translocated animals were trapped without regard to family membership. I measured fitness by retrapping all marked animals remaining at release sites in the summer following release. Family translocated animals were five times more likely to survive and had significantly higher reproductive success than those translocated without families. Predation was an important impediment of translocation success, but family translocation significantly reduced the success of predators on newly established prairie dog colonies. Postrelease survival was also affected by the timing of release, but appeared to be more important for juveniles than adults. These results demonstrate the importance of considering familiarity when translocations are required. More broadly, these results illustrate the value of applying animal behavior to conservation efforts and suggest that other species dependent on social interactions for survival and reproduction may benefit substantially from the maintenance of social groups during translocations.     

The Normative Dimension and Legal Meaning of Endangered and Recovery in the U.S. Endangered Species Act

Abstract:  The ethical, legal, and social significance of the U.S. Endangered Species Act of 1973 (ESA) is widely appreciated. Much of the significance of the act arises from the legal definitions that the act provides for the terms threatened species and endangered species. The meanings of these terms are important because they give legal meaning to the concept of a recovered species. Unfortunately, the meanings of these terms are often misapprehended and rarely subjected to formal analysis. We analyzed the legal meaning of recovered species and illustrate key points with details from "recovery" efforts for the gray wolf ( Canis lupus ). We focused on interpreting the phrase "significant portion of its range," which is part of the legal definition of endangered species. We argue that recovery and endangerment entail a fundamentally normative dimension (i.e., specifying conditions of endangerment) and a fundamentally scientific dimension (i.e., determining whether a species meets the conditions of endangerment). Specifying conditions for endangerment is largely normative because it judges risks of extinction to be either acceptable or unacceptable. Like many other laws that specify what is unacceptable, the ESA largely specifies the conditions that constitute unacceptable extinction risk. The ESA specifies unacceptable risks of extinction by defining endangered species in terms of the portion of a species' range over which a species is "in danger of extinction." Our analysis indicated that (1) legal recovery entails much more than the scientific notion of population viability, (2) most efforts to recover endangered species are grossly inadequate, and (3) many unlisted species meet the legal definition of an endangered or threatened species.     

Conservation Genetics of the Endangered Isle Royale Gray Wolf

Abstract. The small group of wolves on Isle Royale has been studied for over three decades as a model of the relationship between large carnivores and their prey. During the last ten years the population declined from 50 individuals to as few as 12 individuals. The causes of this decline may be food shortages, disease, or reduced genetic variability. We address the issues of genetic variability and relationships of Isle Royale wolves using allozyme electrophoresis, mtDNA restriction-site analysis, and multilocus hypervariable minisatellite DNA analysis (genetic fingerprinting). Our results indicate that approximately 50% of the allozyme heterozygosity has been lost in the island population, a decline similar to that expected if no immigration had occurred from the mainland. The genetic fingerprinting data indicate that the seven sampled Isle Royale wolves are as similar as captive populations of siblings. Surprisingly, the Isle Royale wolves have an mDNA genotype that is very rare on the mainland, being found in only one of 144 mainland wolves ThFF suggests that the remaining Isle Royale wolves areprobably derived from a single female founder     

Grassland Bird Responses to Land Management in the Largest Remaining Tallgrass Prairie

Abstract: Extensive habitat loss and changing agricultural practices have caused widespread declines in grassland birds throughout North America. The Flint Hills of Kansas and Oklahoma—the largest remaining tallgrass prairie—is important for grassland bird conservation despite supporting a major cattle industry. In 2004 and 2005, we assessed the community, population, and demographic responses of grassland birds to the predominant management practices (grazing, burning, and haying) of the region, including grasslands restored under the Conservation Reserve Program (CRP). We targeted 3 species at the core of this avian community: the Dickcissel (Spiza americana), Grasshopper Sparrow (Ammodramus savannarum), and Eastern Meadowlark (Sturnella magna). Bird diversity was higher in native prairie hayfields and grazed pastures than CRP fields, which were dominated by Dickcissels. Although Dickcissel density was highest in CRP, their nest success was highest and nest parasitism by Brown‐headed Cowbirds (Moluthrus ater) lowest in unburned hayfields (in 2004). Conversely, Grasshopper Sparrow density was highest in grazed pastures, but their nest success was lowest in these pastures and highest in burned hayfields, where cowbird parasitism was also lowest (in 2004). Management did not influence density and nest survival of Eastern Meadowlarks, which were uniformly low across the region. Nest success was extremely low (5–12%) for all 3 species in 2005, perhaps because of a record spring drought. Although the CRP has benefited grassland birds in agricultural landscapes, these areas may have lower habitat value in the context of native prairie. Hayfields may provide beneficial habitat for some grassland birds in the Flint Hills because they are mowed later in the breeding season than elsewhere in the Midwest. Widespread grazing and annual burning have homogenized habitat—and thus grassland‐bird responses—across the Flint Hills. Diversification of management practices could increase habitat heterogeneity and enhance the conservation potential of the Flint Hills for grassland birds.     

Spatiotemporal Dynamics of Endangered Species Hotspots in the United States

Abstract: Given limited resources, many researchers advocate focusing conservation efforts on hotspots, geographical areas with high numbers of species (i.e., richness), endemic species, rare or threatened species, and/or high levels of threat to species survival. The hotspot approach is an efficient and simple way to conserve species diversity, assuming that hotspots do not change over space or time. We tested whether hotspots change across space and time using a database of endangered and threatened species listed by the U.S. government from 1967 to 1999. We determined hotspots based on the cumulative set of species listed for three overlapping and successively longer time periods: 1967–1979, 1967–1989, and 1967–1999. We used minimum area complimentarity analysis, which selected the smallest set of areas (in our study, U.S. counties) needed to represent a chosen set of species. Over time, the number of endangered and threatened species in the United States increased from 76 in 1967 to 1123 in 1999. As the number of species increased over time, hotspots changed in two ways: the number of hotspots increased and the rank of hotspots shifted. Hotspots increased from 84 in 1979, to 166 in 1989, to 217 in 1999. Only 63 of these counties were designated as hotspots in all three periods. The remaining changes resulted from addition and deletion of counties as hotspots over time. Some counties were removed from the list or changed in relative rank from one time period to the next regardless of their rank. Counties added as hotspots could rank anywhere on the list, and they were not merely low-ranking counties added to represent one or a few species. Therefore, hotspots serve as a useful tool for guiding conservation efforts but, given their spatiotemporal variability, do not represent a final solution.     

Genetic Structure of Fragmented Populations of the Endangered Daisy Rutidosis leptorrhynchoides

Abstract: The endangered grassland daisy Rutidosis leptorrhynchoides has been subject to severe habitat destruction and fragmentation over the past century. Using allozyme markers, we examined the genetic diversity and structure of 16 fragmented populations. The species had high genetic variation compared to other plant species, and both polymorphism and allelic richness showed strong positive relationships with log reproductive population size, reflecting a loss of rare alleles (frequency of q < 0.1) in smaller populations. Fixation coefficients were positively related to size, due either to a lack of rare homozygotes in small populations or to Wahlund effects (owing to spatial genetic structure) in large ones. Neither gene diversity nor heterozygosity was related to population size, and other population parameters such as density, spatial contagion, and isolation had no apparent effect on genetic variation. Genetic divergence among populations was low , despite a large north-to-south break in the species' current distribution. To preserve maximum genetic variation, conservation strategies should aim to maintain the five populations larger than 5000 reproductive plants, all of which occur in the north of the range, as well as the largest southern population of 626 plants at Truganina. Only one of these is currently under formal protection. High heterozygosity in smaller populations suggests that they are unlikely to be suffering from inbreeding depression and so are also valuable for conservation. Erosion of allelic richness at self-incompatibility loci, however, may limit the reproductive capacity of populations numbering less than 20 flowering plants.     

What Exactly Is an Endangered Species? An Analysis of the U.S. Endangered Species List: 1985–1991

Critics of the Endangered Species Act have asserted that is protects an inordinate number of subspecies and populations, in addition to full species, and that the scientific rationale for listing decisions is absent or weak. We reviewed all U.S. plants and animals proposed for listing or added to the endangered species list from 1985 through 1991 to determine the relative proportion of species, subspecies, and populations, and their rarity at time of listing. Approximately 80% of the taxa added to the list were full species, 18% were subspecies, and 2% were distinct populations segments of more widespread vertebrate species. The proportion of subspecies and populations was considerably higher among birds and mammals than among other groups. The median populations size at time of listing for vertebrate animals was 1075 individuals; for invertebrate animals it was 999. The median population size of a plant at time of listing was less than 120 individuals. Earlier listing of declining species could significantly improve the likelihood of successful recovery, and it would provide land managers and private citizens with more options for protecting vanishing plants and animals at less social or economic cost.     

Limiting Depredation by African Carnivores: the Role of Livestock Husbandry

Abstract:   Most large carnivore species are in global decline. Conflict with local people, particularly over depredation on livestock, is a major cause of this decline, affecting both nominally protected populations and those outside protected areas. For this reason, techniques that can resolve conflicts between large carnivores and livestock farmers may make important contributions to conservation. We monitored rates of livestock depredation by lions (  Panthera leo ), leopards ( Panthera pardus ), cheetahs ( Acinonyx jubatus ), and spotted hyenas ( Crocuta crocuta ), and retributive killing of these species by farmers in livestock-producing areas of Laikipia District, Kenya. Farmers killed more lions, leopards, and spotted hyenas where these predators killed more livestock. Livestock husbandry had a clear effect on rates of depredation and hence on the numbers of predators killed. Cattle, sheep, and goats experienced the lowest predation rates when attentively herded by day and enclosed in traditional corrals (bomas) by night. Construction of the boma, the presence of watchdogs, and high levels of human activity around the boma were all associated with lower losses to predators. Although most of this work was carried out on commercial ranches, local Maasai and Samburu pastoralists have practiced nearly identical forms of husbandry for generations. Our study shows that traditional, low-tech husbandry approaches can make an important contribution to the conservation of large carnivores.