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1.
Some experiments on phosphate assimilation by coastal marine plankton   总被引:2,自引:0,他引:2  
A study of phosphate assimilation by coastal marine plankton revealed that both phytoplankton and microheterotrophs incorporated radioactive phosphorus (33P). Size fractionation of the particulate matter (using 1 m pore diameter Nucleopore® membrane filters), antibiotic treatment (using garamycin), and independent estimaties of photoautotrophic (14CO2 uptake) and heterotrophic (3H-glucose uptake) activities were employed to separate phyto- and bacterioplankton phosphate uptake. Results indicated that phytoplankton 33P-uptake was best estimated by the fraction of particulate matter retained on the 1 m membrane filters. Usually, less than 10% of the phytoplankton (based on chlorophyll a measurements) passed the 1 m pore-diameter filters, whereas about 90% of the heterotrophic activity passed. At least 50% of the 33P-uptake was associated with the <1 m fraction. It may be possible to resolve the phytoplankton and bacterial contributions to 33P-uptake by comparing the percent of total 33P-uptake with the percent of total 3H-glucose uptake associated with the >1 m fraction.  相似文献   

2.
The reef coral Pocillopora damicornis (Linnaeus) was grown for 8 wk in four nutrient treatments: control, consisting of ambient, unfiltered Kaneohe Bay seawater [dissolved inorganic nitrogen (DIN, 1.0 M) and dissolved inorganic phosphate (DIP, 0.3 M)]; nitrogen enrichment (15 M DIN as ammonium); phosphorus enrichment (1.2 M DIP as inorganic phosphate); and 15 M DIN+1.2 M DIP. Analyses of zooxanthellae for C, N, P and chlorophyll a after the 8 wk experiment indicated that DIN enrichment increased the cellular chlorophyll a and excess nitrogen fraction of the algae, but did not affect C cell-1. DIP enrichment decreased both C and P cell-1, but the decrease was proportionally less for C cell-1. the response of cellular P to both DIN and DIP enrichment appeared to be in the same direction and could not be explained as a primary effect of external nutrient enrichment. The observed response of cellular P might be a consequence of in situ CO2 limitation. DIN enrichment could increase the CO2 (aq) demand by increasing the net production per unit area. DIP enrichment could slow down calcification, thus decreasing the availability of CO2 (aq) in the coral tissue.Hawaii Institute of Marine Biology Contribution No. 920  相似文献   

3.
The shortterm (10–22 d) effect of Zn, Hg, Cu, Cd, Pb, and Ni on the length growth of Mytilus edulis is studied. Significant reductions of growth rate was found at 0.3 g Hgl-1, 3 g Cul-1, 10 g Znl-1, and 10 g Cdl-1 added to the local sea water, while concentrations of up to 200 gl-1 of Pb and Ni had no effect on the growth. With exposure to Cu and Zn, there was a linear reduction in growth rate with increasing metal concentration up to about 6 g Cul-1 and 100 g Znl-1. Above these levels, growth stopped with Cu, while with Zn it was stabilized at about 20% of control growth. When Hg and Cd were added, a curvilinear relationship between growth and metal concentration is indicated. With Hg, growth rate is nearly zero above 3–4 g Hgl-1, while the growth rate was 50% of control after 10 d of exposure to 100 g Cdl-1. At 2 g Cdl-1 there was a significant stimulation of length increase. Observed EC50-values for growth were 0.3–0.4 g Hgl-1, 3–4 g Cul-1, 60 g Znl-1, and 100 g Cdl-1.  相似文献   

4.
Michaelis-Menten uptake kinetics were observed at all light intensities. With constant illumination, the Vmax and K1 in nitrate uptake over the natural light intensity range of 0 to 2000 E were 0.343 g-at NO3–N(g)-1 at protein-N h-1 and 26 E, respectively. Nitrate uptake was inhibited at higher light intensities. The Ks for nitrate uptake did not vary as a function of light intensity remaining relatively constant at 0.62 g-at NO3–N 1-1. With intermittent illumination, the Vmzx for light intensity in nitrate uptake over a light intensity range of 0 to 5000 E was 0.341 g-at NO3–N(g)-1-at protein-N h-1. No inhibition of nitrate uptake was observed at higher than natural light intensities. Chaetoceros curvisetus will probably never experience light inhibition of nitrate uptake under natural conditions.  相似文献   

5.
Adult Elasmopus rapax, collected from the eastern coast of Venezuela in 1990, were exposed to seawater containing various CdCl2 concentrations ranging from 0.25 to 5.5 mol l-1. The 48-h and 96-h LC50 values obtained were 4.0 and 1.6 mol Cd l-1, respectively. In amphipods exposed to 1 mol Cd l-1 for up to 240 h, the apparent rate of cadmium uptake was higher in dead animals (most of which had molted during the preceding 24 to 48 h) than in those which survived throughout the treatments without molting. Thus, whole-body cadmium content reached 1.74 mol g-1 dry weight (dw) in the former and only 0.85 mol g-1 dw in the latter; the higher body Cd-load may have caused the increased mortality observed in molters. On exposure to cadmium levels above 0.5 mol l-1 the oxygen consumption rate of non-molters decreased from 2.2 to about 1.5 ml O2 g-1 dw h-1 over the first 24 h, remaining unchanged thereafter. The results place E. rapax among the most sensitive marine organisms yet studied concerning cadmium toxicity, and emphasize the usefulness of the Amphipoda as bioindicators and research tools for bioassays.  相似文献   

6.
Concentrations of thallium in phytoplankton (0.02 to 0.8 g g–1), zooplankton (0.03 to 0.5 g g–1) and ichthyoplankton (0.1 g g–1) from the central Pacific were comparable , as were the atomic ratios of thallium to calcium (3x10–6) and to potassium (1x10–6) in those organisms. These relatively constant ratios, plus the biounlimited ocean profile of thallium, indicate that it is rapidly cycled through plankton in the same manner as potassium, its principal biogeochemical analogue. The higher atomic ratios of thallium to potassium in pelagic clays (6x10–6) and ferromanganese nodules (4x10–3) suggest that both biological transport processes and abiotic transport processes influence this trace element's oceanic cycle.  相似文献   

7.
C. E. Pesch 《Marine Biology》1979,52(3):237-245
Adult male Neanthes arenaceodentata were exposed to 0.10±0.015 mg l-1 copper in the seawater of a continuous-flow bioassay system in the presence of a sand, a mud, a mixture of the sand and mud, and no sediment, to assess the influence of sediment type on Cu-induced mortality. The sediment type did influence mortality. The time to 50% mortality was 7.8 days without sediment, 36.5 days with sand, 54.5 days with the mixture, and 50.0 days with mud. There was no threshold Cu body burden that caused death. The mean Cu concentration per gram of N. arenaceodentata (24 h after death) without sediment was 270 g, in sand 994 g, in the mixture 1047 g, and in mud 1464 g. The differences in the toxic responses are discussed.  相似文献   

8.
The average grazing and ingestion rates of all stages of the marine planktonic copepod Calanus helgolandicus (Calanoida) from nauplius stage IV to adults were measured experimentally at 15°C in agitated cultures. The chain-forming diatom Lauderia borealis and the unarmoured dinoflagellate Gymnodinium splendens were offered as food. The food concentrations were close to natural conditions and ranged from 36 to 101 g of organic carbon per liter. The medium body weights expressed in g of organic carbon of almost all larval stages raised at 49 g C/1 were identical with the weight of the same stages caught in the Pacific Ocean off La Jolla, California, USA. In a log-log system, grazing and ingestion rates increased almost linearly with increasing body weight. Grazing rates ranged from 4 to 21 ml/day/nauplius stage IV to 286 ml to 773 ml/day/female. Ingestion rates increased from 0.2 g to 0.8 g C/day/nauplius stage IV to 18 g to 69 g C/day/female. Grazing and ingestion rates per unit body weight decreased gradually with increasing body weight. The daily ingested amount of food decreased from 292 to 481% of the body weight (g C) of nauplius stage V to 28–85% of the body weight of adult females. Grazing and ingestion performances of all stages increased with increasing particle size. Grazing rates decreased and ingestion rates increased with increasing food concentrations. The published data on food intake of the different age groups of C. helgolandicus show that the young stages of herbivorous planktonic copepods can play a major part in the consumption of phytoplankton in the sea due to their high grazing and ingestion rates.  相似文献   

9.
Nauplii of Calanus pacificus were raised on a mixture of algae. Details of the mouth-parts, such as denticles, labial palps and lobes, setations and structure of the masticatory teeth were examined by scanning electron microscopy (SEM). Under the experimental conditions (15°C and 300 gC l-1), exponential growth coefficients for the period Nauplii II–VI were 0.179 for carbon and 0.228 for nitrogen. C:N ratios dropping from 5.1 to 4.7. Growth was isochronous, each stage lasting 1.5 days. Respiratory losses were 15 to 19.6% of body carbon daily. Nauplii raised on a given alga showed higher rates of ingestion in the presence of this food, compared to nauplii switched to other algae. Minimal threshold concentrations for feeding were found, depending on the size of the food offered and ranging from 5.8 gC I-1 for Lauderia borealis (28.7 m spherical diameter) to 47.1 gC 1-1 for Chlamydomonas sp. (11.0 m). Unlike the Copepodite I stage, Nauplii II–VI larvae were not able to ingest small cells such as Isochrysis galbana (4.3 m), or very large ones such as Ditylum brightwellii (47.5 m) at more than maintenance rations. Below the critical concentration for maximal feeding, ingestion was clearly dependent on size of the cells offered, but the size-dependent relationship was different for diatoms and non-diatoms. Filtering rates increased from a threshold concentration to a maximal rate at about 50 gC 1-1, and decreased at higher concentrations. Critical concentrations ranged from 125 gC 1-1 for L. borealis to 1000 gC 1-1 for Chlamydomonas sp. Maximal daily rations ranged between 100 and 150% of body carbon.  相似文献   

10.
Soil and water samples were collected from the Susaki area of Korinthos and analysed for heavy metals in order to evaluate their environmental impact. The geology of the studied area includes ultrabasic rocks and Neogene and Quaternary deposits whereas magnesite veins are found within the ultrabasic rocks. In the north part of the studied area post volcanic emissions of H2S, CO2 and H2O vapor continue to the present day. All the samples were analysed for heavy metals by the ICP method. The element ranges (in g g–1) for soil samples are: Cu 11–63, Pb 5–256, Zn 21–604, Ni 183–2665, Co 12–124, Mn 456–1434, As 5–104, Sr 44–730, V 21–84, Cr 163–2346, Ba 48–218, Zr 3–41, Y 3–13. The metals Pb, Zn, Ni, Co, Cr, Fe, Cu, Mn, As and Sr are enriched in the Susaki soils. The element ranges for water samples are: Cu 65–103ppb, Pb<10ppb, Zn<5ppb, Ni 21–163ppb, Co 2–12ppb, As<30ppb, Cr<20ppb, Ba 36–785ppb, Sb<10ppb, W<10ppb, Bi<30ppb, Mn 0.0–0.9 g g–1, Fe 0.01–0.22 g g–1, Na 843–3076 g g–1, K 98–278 g g–1, Si 39–65 g g–1, P 0.1–0.2 g g–1. There is a natural pollution of soils with elevated concentrations of Ni, Co, Mn, Fe and Cr due to the presence of ultrabasic rocks. Another natural case of As pollution of soils is due to the volcanic activity and the geothermal field in the area. The geochemical data of ground waters and also the 18, D data showed a mixing in different proportions between sea water and meteoric water.  相似文献   

11.
The distribution of cyanobacteria in the surface waters of the North Sea was measured during July 1987. Numbers of cyanobacteria ranged from 2.5x106 to 1.7x108 cells 1-1. In the majority of stations, cyanobacterial numbers were highest in the near-surface water and a subsurface maximum was found at only one station. The distribution of 14C among the end-products of photosynthesis was determined for picoplankton (<1 m) and other phytoplankton >1 m throughout the North Sea. The majority of label was found in the protein fraction of both picoplankton and >1 m phytoplankton; incorporation into lipids and polysaccharides plus nucleic acids was much lower. We interpret the large incorporation into protein to be a consequence of nutrient limitation of these natural assemblages. Photosynthetic parameters of the two size fractions were also determined. Assimilation number (P m B ) and initial slope were greater for the picoplankton fraction than for phytoplankton >1 m but there was no evidence of significant photoinhibition of either fraction at irradiances up to 1 000 E m-2 s-1.  相似文献   

12.
Uptake of cadmium by the eelgrassZostera marina   总被引:1,自引:0,他引:1  
The uptake of cadmium by the shoots ofZostera marina L. (eelgrass) was examined in the laboratory. Experiments were carried out in experimental chambers which allowed the separation of the leaves from the root-rhizome portions of intact shoots. Cadmium uptake by the root-rhizome portions over 24 h was directly related to substrate cadmium concentration (1.0, 5.0 and 10.0 g Cd ml-1) and varied from 6.5 to 30.0 g Cd g-1. Cadmium uptake by the root-rhizomes and the leaves in a substrate concentration of 1 g Cd ml-1 was also related to exposure times (24, 48 and 72 h). Maximum uptake by the root-rhizomes and the leaves was observed after 72 h at a substrate concentration of 1 g Cd ml-1 and was equivalent to 48 and 94 g Cd g-1, respectively. Translocation of cadmium from the leaves to the root-rhizomes was observed after 24 h, and at the end of 72 h was equivalent to 27% of the total leaf uptake. No cadmium movement from the root-rhizomes to the leaves was detected.  相似文献   

13.
The feeding behaviors of Acartia clausi and A. tonsa were measured in samples of water containing low levels of a water-accommodated fraction of No. 2 fuel oil. The copepods fed normally at a hydrocarbon concentration of 70 g l-1, but their feeding behavior was altered both quantitatively and qualitatively at a concentration of 250 g l-1. Three types of response to the higher oil level were found. The first was total suppression of feeding. Both other types involved suppression of feeding on particles between 7 and 15 m diameter, but one showed no change in the ingestion of larger particles, whereas the other displayed increased feeding on particles larger than 15 m diameter. These results suggest that the species of Acartia studied use three different modes of feeding, each on a different size range of particulate material. Low-level hydrocarbon pollution affects each feeding mode differently.Contribution No. 973, Center for Environmental and Estuarine Studies of the University of Maryland  相似文献   

14.
The distribution of phytoplankton primary production into four size fractions (>10 m, 10-3 m, 3-0.2 m and <0.2 m), the utilization of algal exudates by bacteria and the bacterial production were studied in a eutrophication gradient in the northern Baltic proper. The polluted area exhibits substantially increased nutrient, especially nitrogen, levels while only minor differences occur in salinity and temperature regimes. Total primary production was 160 g C · m-2 · yr-1 at the control station and about 275 g C · m-2 · yr-1 at the eutrophicated stations. The estimated total exudate release was 16% of the totally fixed 14CO2 in the control area and 12% in the eutrophicated area (including the estimated bacterial uptake of exudates). The difference in14CO2 uptake rates between incubation of previously filtered water (<3, <2, <1 m) and unfiltered water was used to estimate bacterial uptake of phytoplankton exudates which were found to contribute about half of the estimated bacterial carbon requirement in both areas. Bacterial production was estimated by the frequency of dividing cells (FDC) method as being 38 g C · m-2 · yr-1 at the control station and 50 g C · m-2 · yr-1 at the eutrophicated stations. To estimate the mean in situ bacterial cell volume a correlation between FDC and cell volume was used. The increased annual primary production in the eutrophicated area was due mainly to higher production during spring and autumn, largely by phytoplankton cells (mainly diatoms) retained by a 10 m filter. Primary production duringsummer was similarin the two areas, as was the distribution on different size fractions. This could possibly explain the similar bacterial production in the trophic layers at all stations since the bulk of bacterial production occurs during summer. It was demonstrated that selective filtration does not quantitatively separate photoautotrophs and bacteria. A substantial fraction of the primary production occurs in the size fraction <3 m. The primary production encountered in the 3-0.2 m fraction was due to abundant picoplankton (0.5 to 8 · 107 ind · l-1), easily passing a 3 m filter. The picoplankton was estimated to constitute up to 25% of the total phytoplankton biomass in the control area and up to 10% in the eutrophicated area.  相似文献   

15.
Despite some progress in reducing the average lead level in the USA, the streets of Cincinnati, Ohio, are still contaminated by heavy metals. High levels of heavy metals will have significant unequivocal ecological impacts and pose a potential health hazard. This study evaluates the level of heavy- metal contamination in household dust and examines its relationships with the external environment. Samples of outdoor and indoor dust were collected from middle-income residential homes in the Greater Cincinnati Metropolitan District and the metal content was analysed by inductively coupled plasma atomic emission spectrophotometry. Results showed that the mean levels of lead in outdoor and indoor dusts are 650 g g-1 and 377 g g-1 and the copper levels are 253 g g-1 and 510 g g-1, respectively. The median levels are 156 g g-1 and 139 g g-1 for outdoor and indoor lead dusts and 35 g g-1 and 124 g g-1 for outdoor and indoor copper dusts. The degree of contamination may be ascribable to the age of the dwelling unit and the neighbourhood, the time when the unit was last painted, the presence of pets and the type of space heating. In addition, car exhausts seems to be a possible source of contamination.  相似文献   

16.
I. Laing 《Marine Biology》1985,85(1):37-41
Batch cultures of the marine unicellular centric diatom Chaetoceros calcitrans (Paulsen) Takano were maintained by serial subculturing every 4 d into nutrient-enriched natural sea-water medium supplemented with 350, 950 and 1 400 g-at Si l-1. The diatom cultures removed initial silica concentrations of 350 and 950 g-at l-1 from the medium within 2 and 3 d, respectively. About 30 g-at l-1 of the highest initial concentration remained in the medium after 4 d. The mean final cell density with an enrichment of 350 g-at Si l-1 was 3.43±0.26×104 cells l-1 (median cell volume = 77.5±5.0 m3); with 950 g-at Si l-1, 8.55±0.55×104 cells l-1 (50.0±4.5 m3); and with 1 400 g-at Si l-1, 9.72±0.48×104 cells l-1 (37.3±5.0 m3). There was no significant difference in the final total organic weight of cells produced, which was in the range of 170 to 190 mg per 250 ml culture. This consisted of proportionately more lipid and carbohydrate and less protein from the treatment with 350 g-at Si l-1 than from the 1 400 g-at Si l-1 treatment.  相似文献   

17.
A unique data set from lead risk assessments performed on 67 public housing developments from across the United States was made available for analyzes. The data set includes results of lead analysis from 5906 dust wipes and from 1222 soil samples. A total of 487 dwelling units in these developments, as well as associated common areas, were sampled, all by the same team of inspectors. The number of dwelling units within a development that were sampled reflected the guidelines then in force, the 1990 Interim HUD Guidelines, rather than those specified in the 1995 Guidelines. Median dust lead loadings for floors, 151gm–2 (14gft–2), and window sills, 936gm–2 (87gft–2), were much less than former HUD limits of 1076gm–2 (100gft–2) and 5380gm–2 (500gft–2), respectively and are only about one-third of the recently established limits of 431gm–2 (40gft–2) and 2690gm–2 (250gft–2). In contrast, the median lead loading for window troughs, 8560gm–2 (795gft–2), was almost identical to the HUD clearance limit of 8610gm–2 (800gft–2). There was a strong positive correlation between floor and window trough lead loading values for samples from the same dwelling units and those from common areas of the housing developments. Door threshold samples, which may reflect conditions exterior to the dwelling unit, were collected from 53 dwelling units. Median lead loading levels of these samples were more than ten times higher than those in floor samples from the same dwelling units, were about the same as window sill samples and about one-half of levels in window trough samples. Composite sample results, simulated by averaging results from four samples within a dwelling unit, revealed that in order to have the same rate of excedence of standards, the composite standards would have to be reduced, for example, from the single sample value of 1076gm–2 (100gft–2) to 527gm–2 (49gft–2) for floor samples and from the single sample value of 8610gm–2 (800gft–2) to 5160gm–2 (479gft–2) for window troughs. For this public housing data set, the portion of the units in developments containing more than 225 units which exceeded the established limit for window samples was the same when using either the full data set or a random one-half of the data set. This suggests that, for this data set, the number of dwelling units sampled was excessive . Thus, the required increase in the number of dwelling units to be sampled specified in the 1995 Guidelines for developments with more than 225 dwelling units, may not have been necessary if this data set is representative of public housing developments in the United States.  相似文献   

18.
A method is described for the incubation of undisturbed sediment cores under in situ conditions with the addition of low concentrations of 14C-glucose. Data are presented for respiration, gross uptake and actual uptake rate of glucose by bacteria in sandy, wave-washed beaches of the Baltic Sea. On the average, the bacteria respired 8% of the total glucose taken up. The gross uptake measured was between 2.3×10-3 and 6.8×10-3 g 14C-glucose g sediment-1 (dry weight) h-1 (average 4.7×10-3 g g-1 h-1). Minima in the gross uptake rate corresponded with maxima in the concentration of natural free dissolved glucose. For the actual uptake rate, however, very similar uptake rates were calculated for the sediments examined (between 1.4×10-1 and 1.9×10-1 g glucose g-1 h-1, average 1.7×10-1 g g-1 h-1).Publication No. 183 of the Joint Research Program at Kiel University (Sonderforschungsbereich 95 der Deutschen Forschungsgemeinschaft).  相似文献   

19.
Growth and photosynthetic properties of the marine dinoflagellate Amphidinium carterae Hulbert were examined under continuous illumination in batch cultures at four different irradiances between 2 and 150 E m-2 s-1. The slope of both cell- and Chl a-based photosynthesis versus the irradiance curves was greatest for cells grown at 15 E m-2 s-1. The relative Chl a values cell-1 were 1, 1.5 and 2 for cultures grown at 150, 80 and 15 E m-2 s-1, respectively. A low-temperature (-196°C) fluorescence technique was used to examine cells for photoinhibiton. Photoinhibition was greatest for cells grown at 150 E m-2 s-1. However, significant photoinhibition of this species was noted even at 80 E m-2 s-1. No significant difference in the fluorescence pattern was found between cells grown at 2 and 15 E m-2 s-1. Time course studies indicate that photoinhibition may occur within 2 h following exposure to 350 E m-2 s-1 in cells grown at 15 E m-2 s-1 and is reversible when light levels are lowered within 4 h. The ecological significance of phytoplankton unable to cope with excess photosynthetic excitation energy is discussed.  相似文献   

20.
Specimens of the oceanic decapod Systellaspis debilis were collected from six sites in the East Atlantic Ocean between 1970 and 1984, and were analysed for Mn, Fe, Cu, Zn and Cd. The data confirm that there are small but significant differences in mean metal concentrations from some sites which showed no obvious pattern in relation to geographic location of the samples. As a result, ranges of site means are quoted as baseline levels for each metal (g g-1 dry wt): 2.3 to 2.9 g Mn g-1, 31.2 to 77.8 g Fe g-1, 25.9 to 83.4 g Cu g-1, 41.9 to 92.9 gZn g-1, 11.1 to 31.8 g Cd g-1. The concentration of cadmium in S. debilis from all sites was raised relative to cadmium concentrations reported for coastal decapods, perhaps as a result of dietary enrichment. Metal accumulation may provide useful information for understanding the complex feeding behaviour of many oceanic animals.  相似文献   

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