A quantitative study of worker reproduction in honey bee colonies

Summary In 11 Apis mellifera colonies with laying queens, about 0.12% of the males produced derived from eggs laid by workers. This result requires explanation both of why workers produce any males, and, since they do, why they produce so few. Workers may maximize their inclusive fitness by forgoing reproduction, or their sterility may be due to to enforcement of the interests of the queen or those of other workers. The presence of laying workers might then result from developmental noise in the workers, from a failure of communication of the queen's presence, or a failure of enforcement mechanisms. Selection for worker reproduction in colonies following queen loss may also play a role in shaping worker reproduction in colonies with a queen. The hypothesis of worker sterility enforced by other workers seems most likely to be correct, but further studies on these hypotheses are needed.     

A quantitative genetic model of altruistic selection

Summary A quantitative genetic model for evolution by altruistic selection based on family selection models used in agricultural genetics is presented. A quantitative genetic version of Hamilton's rule is derived which indicates that evolution will proceed in an altruistic direction whenever the ratic of between-to within-family (or group) heritability exceeds the absolute value of the within-to between-family selection differential ratio. This ratio of heritabilities is equal to a ratio which includes only the phenotypic and genetic intraclass correlation coefficients, thus no heritabilities actually need to be estimated in determining the possibility of altruistic evolution. The phenotypic intraclass correlation can be estimated with standard analysis of variance methods. The genetic intraclass correlation may be estimated by the average within-group coefficient of relationship using genealogical data, by Wright's F-statistics using allele frequency data, or by a theoretical model based on a knowledge of interaction and mating patterns. Selection differentials may be measured by the regression of relative fitness, or one of its major components, on the phenotype of interest. Selection can be considered altruistic only if within-and between-family selection differentials have opposite signs. Consideration of empirical data on genetic and phenotypic intraclass correlations indicates that evolution may proceed in an altruistic direction even if the within-family selection differential exceeds the one between families, especially in situations in which pertinent environmental factors are randomly distributed among families.     

Fish assemblages composition in a natural, then regulated, stream: A quantitative long-term study

This paper tests the hypothesis how effectively fish assemblage composition was shaped by local climate changes and by engineering impact in 1989. This was possible due to the monitoring protocols of the present study, which allowed estimation of the magnitude of anthropogenic changes from changes naturally occurring in nature. Fish were sampled at the end of every growing season (October) for 23 years at five contiguous sites in a stream, before (1979-1988) and after (1989-2001) regulation. In each sample, six successive electrofishing passes were used to calculate the density and mean biomass for assemblage analysis using the Zippin model. During the study, the natural, meandering stream with pools, riffles, and a moderate canopy was modified into a straight stream of uniform width and depth, stripped of all vegetation. The output layer of a self-organizing map (SOM, the artificial neural network algorithm) applied in this study for site similarity analysis was partitioned into six subclusters placed in two main clusters. Subclusters in the upper part of the SOM were occupied chiefly by regulated stream samples and those in the lower part of the SOM by natural stream samples. Subclusters in the middle position, contained both natural (19) and regulated (20) samples in nearly equal proportion. In addition, the SOM contained one subcluster with sites only from the regulated period and another with only natural sites. Differences between subclusters were attributed to differences in climate, with some differences profound. Warming of the local weather, which became most evident in the 1990s, may have resulted in changes in fish assemblages. This is shown in the SOM, in which samples from the 1980s with cold years dominate the bottom of the SOM, whereas those from the 1990s and later are at the top. Subclusters dominated by regulated or natural sites were not always significantly different when the number of species and diversity indices were considered. Clear differences between the regulated and natural samples involved qualitative characteristics and mainly concerned assemblage composition. They were also confirmed by significant IndVal values (indicator species) and neither mixed subcluster contained important species in their assemblages.     

A quantitative global review of species population monitoring

Species monitoring, defined here as the repeated, systematic collection of data to detect long-term changes in the populations of wild species, is a vital component of conservation practice and policy. We created a database of nearly 1200 schemes, ranging in start date from 1800 to 2018, to review spatial, temporal, taxonomic, and methodological patterns in global species monitoring. We identified monitoring schemes through standardized web searches, an online survey of stakeholders, in-depth national searches in a sample of countries, and a review of global biodiversity databases. We estimated the total global number of monitoring schemes operating at 3300–15,000. Since 2000, there has been a sharp increase in the number of new schemes being initiated in lower- and middle-income countries and in megadiverse countries, but a decrease in high-income countries. The total number of monitoring schemes in a country and its per capita gross domestic product were strongly, positively correlated. Schemes that were active in 2018 had been running for an average of 21 years in high-income countries, compared with 13 years in middle-income countries and 10 years in low-income countries. In high-income countries, over one-half of monitoring schemes received government funding, but this was less than one-quarter in low-income countries. Data collection was undertaken partly or wholly by volunteers in 37% of schemes, and such schemes covered significantly more sites and species than those undertaken by professionals alone. Birds were by far the most widely monitored taxonomic group, accounting for around half of all schemes, but this bias declined over time. Monitoring in most taxonomic groups remains sparse and uncoordinated, and most of the data generated are elusive and unlikely to feed into wider biodiversity conservation processes. These shortcomings could be addressed by, for example, creating an open global meta-database of biodiversity monitoring schemes and enhancing capacity for species monitoring in countries with high biodiversity. Article impact statement: Species population monitoring for conservation purposes remains strongly biased toward a few vertebrate taxa in wealthier countries.     

邻近油砂活动的鱼类性能指标：对邻近油砂开采活动的阿尔伯塔省Steepbank河中粘杜父鱼性能指标的响应

自2009年以来,加拿大和阿尔伯塔省政府一直在制定Athabasca河下游及其支流（2010-2013年）地表水质量和水量的监测计划。这项对鱼类监测计划的贡献的目标是1）评估该河支流中鱼类的当前状态,2）识别上游参考物和油砂沉积物暴露位点之间存在的差异,和3）识别鱼类性能指标相对于历史研究的趋势和变化。本研究考察了阿尔伯塔省Steepbank河中粘杜父鱼（Cottus cognatus）在生长、性腺大小、生长状况和肝脏7-乙氧基甲氧基甲酰胺基脱氢酶（EROD）活性方面的鱼类性能指标,以此作为暴露于油砂相关化合物的指标。采样程序遵循历史采样方法（1999-2000年）,随着开发的进展,随着时间的推移提供可比数据,同时增加一个上游参考位点（n=2）。2010年至2013年,从油砂矿床（n=2）内的Steepbank河下游收集的杜父鱼中记录到了一致的变化。杜父鱼的数据显示,肝脏增大的同时相应的EROD活性诱导与历史数据一致,与生殖发育和性腺类固醇生产能力相关的能量投资减少。没有一致的证据表明,随着露天采矿开发的增加使得鱼类性能指标发生了变化,特别是在Steepbank河中段附近。尽管在过去15年里,Steepbank河流域的物理开发有所增加,但这些结果与历史数据一致,表明开发区附近的水环境的响应程度没有改变。     

A comparison of SEM-EDS with ICP-AES for the quantitative elemental determination of estuarine particles

Suspended particulate matter (SPM) is a key component regulating the biogeochemistry of natural and contaminant moieties in estuaries. Individual particle analyses can complement conventional bulk analyses of SPM, but are rarely undertaken. This study used scanning electron microscopy-energy dispersive X-ray spectrometry (SEM-EDS) of particles to quantify a range of elements in the reference estuarine sediment PACS-2. This approach was compared with a bulk SPM analysis based on inductively coupled plasma-atomic emission spectrometry (ICP-AES). The median concentrations of Al, Fe, Mg, and Ca for the two approaches were similar, and accuracy for both methods was good. SEM-EDS analysis was also satisfactory for K. Agreement was poorer for Mn and Ti, which were present at trace concentrations. Increasing the number of particles examined by SEM-EDS should improve the analysis. SEM-EDS analysis of SPM from the Tamar Estuary, UK, revealed marked geochemical differences between particle sub-populations.Selected article from 6th European Meeting on Environmental Chemistry, Belgrade, 2005, organised by Prof. Dr. Branimir Jovancicevic and the European Association of Chemistry and the Environment (ACE, www.research.plymouth.ac.uk/ace)     

A quantitative comparison of the commonly used methods for extracting carotenoids from avian plasma

Interest in animal carotenoids, especially in birds, has exploded in recent years, and so too have the methods employed to investigate the nature and function of these pigments. Perhaps the most easily and commonly performed procedure in this work has been the determination of carotenoid concentration from avian plasma. Over the past 20 years of research on avian carotenoids, numerous methods have been used to extract carotenoids from bird plasma, all of which have differed in several important parameters (e.g., number and types of solvents used, degree of mixing/centrifugation). However, to date, no study has systematically compared these methods to determine if any of them are more effective than others for recovering any or all types of carotenoids present. We undertook such an investigation on plasma samples from two bird species (house finch, Carpodacus mexicanus, and mallard, Anas platyrhynchos) using five of the most commonly employed methods for extracting carotenoids from avian plasma: (1) acetone-only, (2) methanol-only, (3) ethanol-only, (4) ethanol + hexane, and (5) ethanol + tert butyl methyl ether. We also manipulated the amount of time that extracts were centrifuged, which has varied tremendously in previous studies, to evaluate its importance on carotenoid recovery. We found that all methods equally recovered the polar xanthophylls (lutein and zeaxanthin), but that the methanol-only procedure poorly recovered non-polar carotenoids (less β-carotene in both species and less β-cryptoxanthin in house finches) compared to the other methods. These results suggest that the data accumulated to date on xanthophyll plasma carotenoids in birds should be comparable across studies and species despite the different chemical extraction methods used. However, care should be taken to use relatively strong organic solvents for fully recovering non-polar carotenoids. We also found no effect of centrifugation duration (1 vs. 10 min at 10,000 rpm) on carotenoid recoveries, demonstrating that researchers can save considerable time by centrifuging for a much shorter time period than is typically used.     

A new quantitative analysis of ovarian development in echinoderms: the maturity stage index

This study developed an objective quantitative method for detecting small-scale temporal or spatial differences in gametogenesis in echinoderms. The method was applied to conventional monthly samples of the planktotrophic brittle star, Ophiopholis aculeata, collected at a single site in Newfoundland (eastern Canada) at 10–15 m depth. The samples were analysed to determine gonad index, oocyte size and gonadal stage using histology. The maturity stage index (MSI) was developed to integrate a measure of brittle star size (disc diameter), oocyte size and oocyte density. The MSIs ranged from 0 to 800 and had significantly different means among the four gametogenic stages (early growth, growth, mature and spent). The MSI was more sensitive in revealing significant differences between consecutive stages than any of its individual constituents. The MSI was also applied to gametogenic data from the lecithotrophic holothuroid, Mesothuria lactea, again revealing significant differences between successive oogenic stages. This method is expected to be useful in field and experimental studies of gametogenesis in echinoderms (and possibly other taxa), where it is important to detect not just the timing of annual peaks in reproduction but small differences in reproductive status among individuals or populations (e.g. from different habitats or feeding regimes).     

A general random walk model for the leptokurtic distribution of organism movement: Theory and application

The movement of organisms is usually leptokurtic in which some individuals move long distances while the majority remains at or near the area they are released. There has been extensive research into the origin of such leptokurtic movement, but one important aspect that has been overlooked is that the foraging behaviour of most organisms is not Brownian as assumed in most existing models. In this paper we show that such non-Brownian foraging indeed gives rise to leptokurtic distribution. We first present a general random walk model to describe the organism movement by breaking the foraging of each individual into events of active movement and inactive stationary period; its foraging behaviour is therefore fully characterized by a joint probability of how far the individual can move in each active movement and the duration it remains stationary between two consecutive movements. The spatio-temporal distribution of the organism can be described by a generalized partial differential equation, and the leptokurtic distribution is a special case when the stationary period is not exponentially distributed. Empirical observations of some organisms living in different habitats indicated that their rest time shows a power-law distribution, and we speculate that this is general for other organisms. This leads to a fractional diffusion equation with three parameters to characterize the distributions of stationary period and movement distance. A method to estimate the parameters from empirical data is given, and we apply the model to simulate the movement of two organisms living in different habitats: a stream fish (Cyprinidae: Nocomis leptocephalus) in water, and a root-feeding weevil, Sitona lepidus in the soil. Comparison of the simulations with the measured data shows close agreement. This has an important implication in ecology that the leptokurtic distribution observed at population level does not necessarily mean population heterogeneity as most existing models suggested, in which the population consists of different phenotypes; instead, a homogeneous population moving in homogeneous habitat can also lead to leptokurtic distribution.     

A little movement orientated to the geomagnetic field makes a big difference in strong flows

Whilst a range of animals have been shown to respond behaviourally to components of the Earth’s magnetic field, evidence of the value of this sensory perception for small animals advected by strong flows (wind/ocean currents) is equivocal. We added geomagnetic directional swimming behaviour for North Atlantic loggerhead turtle hatchlings (Caretta caretta) into a high-resolution (1/4°) global general circulation ocean model to simulate 2,925-year-long hatchling trajectories comprising 355,875 locations. A little directional swimming (1–3 h per day) had a major impact on trajectories; simulated hatchlings travelled further south into warmer water. As a result, thermal elevation of hatchling metabolic rates was estimated to be between 63.3 and 114.5% after 220 days. We show that even small animals in strong flows can benefit from geomagnetic orientation and thus the potential implications of directional swimming for other taxa may be broad.     

Polimetrics: the quantitative study of urban systems (and its applications to atmospheric and hydro environments)

The rapid urbanization of the Earth has led to highly populated cities that act as concentrated centers of anthropogenic stressors on the environment. Much of these stressors originate from nonlinear coupling between man-made urban elements (i.e., networks of engineering and socio-economic infrastructures) and the natural environment. An urban area can be treated as a system of urban elements (or elemental systems), and simple interactions between the latter may lead to complex outcomes, known as emergent properties, in the former. Emergent properties of urban systems play an important role in determining their resilience and sustainability, studies on which require an in-depth knowledge of dynamics, interdependencies and feedbacks of urban elements as well as development of quantitative holistic models to integrate such knowledge to predict the overall system response. To facilitate this demanding effort, it is proposed to initiate a new sub-discipline within the discipline of Earth System Engineering, with the portmanteau of Polimetrics, to deal with quantitative scientific aspects of urban systems. This paper discusses two examples of polimetrics related to urban fluid flows. The first deals with the urban heat island and the other with storm surges in coastal cities.     

A simple density separation technique for quantitative isolation of meiobenthos using the colloidal silica Ludox-TM

A simple, quantitative density separation method is described. The method is based on differences in specific weight between meiobenthos and sediment. Nematodes and copepods could be separated from sediment and detritus when samples were suspended in Ludox-TM, a colloidal silica. Organisms float at the surface, while sediment particles sink. Results obtained with this new method were compared with the well-known decantation method. For a quantitative isolation of nematodes from sediments, rich in coarse detritus, a maximum volume of 7 cm³ sample could be used. For copepods this maximum was 13 cm³. For such sediments the density method is more reliable than the decantation method. The time needed for sorting the meiobenthic organisms is reduced to about 30% compared with the former method. The new method can be used for preserved as well as for fresh sediment samples and can also be applied for the disolation of small polychaetes, small oligochaetes, larvae of some macrofaunal groups and net-zooplankton.Publication No. 10 of the project Biological Research in the Ems-Dollard Estuary.     

Feeding and movement patterns of the sea cucumber Holothuria sanctori

Sea cucumbers are conspicuous organisms inhabiting almost all marine habitats across the globe. Despite their importance as effective consumers of detritus, little is known about their behavior. We studied organic matter (OM) consumption and nocturnal movement patterns of Holothuria sanctori through indoors assays and in situ experiments at shallow bottoms off Gran Canaria Island (Canary Islands, Spain). H. sanctori has a selective feeding toward high OM concentration sediments. Consumption of OM increased with OM availability, particularly during formation of the gonads. OM consumption did not differ in situ between two adjacent habitats arranged in mosaics: macroalgal beds and urchin-grazed “barrens.” Larger distances and fastest displacements were covered by H. sanctori during the end than the middle and start of the nighttime. Overall, we did not detect a clear “homing” behavior by H. sanctori, yet some individuals showed a fidelity for refuges when shelter availability was lacking.     

Experimental examination of movement of the giant scallop,Placopecten magellanicus

We quantified movements of tagged giant scallops, Placopecten magellanicus (Gmelin, 1791), (40 to 115 mm in shell height) released in 1991 and 1992 at nine stations in Port Daniel Bay, Baie des Chaleurs, Gulf of St. Lawrence, Canada, for periods of 10 to 52 d. Both the mean distances moved per day and dispersion distances from release points were usually greater at the two stations with sand substratum, low scallop densities and high rock crab, Cancer irroratus, densities. Movement rate and dispersion of scallops from the release points at the other seven stations were similar, even though there were marked differences in substratum type (gravel, cobble or bedrock), predator density, and scallop density. Most mean dispersal directions were random, and scallops did not appear to migrate from unsuitable to suitable habitats. Although movement did reduce predation rate, scallop movement was weakly correlated with the abundance of only one predator, C. irroratus.     

A fast and reliable method for quantitative determination of total mercury in vegetables

A cloud-point extraction (CPE) process using the nonionic surfactant, polyethylene glycol tert octylphenyl ether (Triton X-114) was employed for determination of Hg(II) ions in aqueous solutions. The method is based on the ion-pairing reaction of Hg(II) with Pyronin B (PyrB⁺) in the presence of excess iodide at pH 6.0 and extraction of the complex formed. The chemical variables affecting CPE efficiency were studied, and the analytical characteristics of the method were obtained. The calibration curves were linear in the range of 1–40 μg L^?1 with the detection limits of 0.35 and 0.30 μg L^?1 at 556 and 521 nm. Selectivity was also tested. The coefficients of variation of the method are 2.4% and 5.2% for five replicate measurements of mercury at levels of 10 and 25 μg L^?1, respectively. The results obtained for two certified reference samples were in a good agreement with the certified values. The method was applied to the determination of total mercury in vegetable samples.     

Laboratory and field evidence of sex-biased movement in the invasive round goby

Activity levels are modulated by trade-offs between reducing predation risk and the need to move in order to find food or mates. Because these trade-offs affect males and females differently, many species show sex-specific movement, dispersal patterns, and spatial navigation capacities, with the sex that gains the most from territory ownership often dispersing less. Unlike mammals and birds, sex differences in movement among fishes remain poorly studied, and the connections between tests of movement propensity in the laboratory and in the field are rarely made. Here, we examine the differences in movement between male and female round goby (Neogobius melanostomus) in both laboratory and field settings. This fish species is invasive in North America and currently undergoing further range expansions. In the laboratory, round goby males were more active and explored a novel environment more readily than did females. A large-scale mark–recapture study in Lake Ontario over two years revealed that males moved more than females between years, but there were no within-year sex differences. Thus, round goby display male-biased movement patterns, providing a comparison point to dispersal patterns in other taxa. Understanding sex-specific movement of round goby in the field will also help predict dispersal and population dynamics, both in areas where round goby have already become established and where they are continuing to invade.     

Sensitivity analysis of the asymptotic behavior of a model for the environmental movement of radionuclides

The asymptotic behavior of a linear compartment model for the environmental movement of radionuclides is investigated. Here, the expression asymptotic behavior is used to designate the behavior of q(t) as t → ∞, where q is the solution of a vector differential equation of the form dq/dt = h + Kq. The asymptotic behavior of such equations is described. For the model and conditions under consideration, each element of q converges monotonically to a steady-state value. A hydrologic system is defined and used to illustrate this behavior. An approach to sensitivity analysis employing Latin hypercube sampling, rank transformations and stepwise regression is presented and then applied to this system. A total of 20 independent variables is introduced and the following dependent variables are investigated for the various components of the system: amount of radionuclide present at steady state. concentration of radionuclide at steady state, and time required to reach 90% of steady state. Finally, an application of asymptotic behavior in the analysis of a hypothetical site for the geologic isolation of high-level radioactive waste is described and a brief discussion of differential sensitivity analysis is given.