Ecophysiological indicators of microbial biomass status in chernozem soils of the Central Caucasus (in the territory of Kabardino-Balkaria with the Terek variant of altitudinal zonation)

A comparative assessment of the biological properties of chernozem soils in agro- and biogeocenoses has been made by determining the rates of basal and substrate-induced soil respiration and using these data to calculate ecophysiological indices characterizing the potential and stability of soil microbial biomass. The results show that the rate of CO₂ emission and the contents of microbial biomass carbon in agriculturally exploited chernozem soils have decreased by factors of 3 and 2.6, respectively. The values of microbial metabolic quotients are indicative of medium-to-strong disturbance to the stability of microbial communities in chernozem soils of agrocenoses.     

Effects of heavy metal pollution on the ecological and biological characteristics of common chernozem

A detailed comprehensive study on the effects of heavy metals on the biological activity and other characteristics of common chernozem of the southern European facies was performed. This involved the analysis of various microbiological and biochemical indices characterizing soil biological activity, their dynamics, a set of several metals and their different chemical forms, and a wide range of metal concentrations in the soil. New important aspects of the effects of heavy metals on biological processes in the soil were revealed. An integral method was proposed for assessing changes in the total biological activity of the soil on the basis of informative indices used in the monitoring, diagnosis, and indication of soils polluted with heavy metals. Geographic trends in changes of soil resistance to heavy metal pollution were analyzed within the common chernozem subtype and by comparing chernozem with other soils.     

Ecological Features of Green Strawberry Cenopopulations in the Steppe Transvolga Region

The results of field studies at the Krasnosamarskii Station of Samara State University (the Samara River valley, the subzone of herb–sheep's fescue–feather grass steppes on ordinary chernozem) have shown that the age structure, ecological density, age index, and regeneration capacity of green strawberry cenopopulations depend on the trophic level and moisture content of soil and on the shading and mulching action of litter.     

Effect of phytoremediation in technogenic landscapes of mining areas on the functioning of actinomycete communities

It has been shown that conditions formed in technozems of tailing dumps covered with a layer of chernozem are optimal for the development of soil microflora, including actinomycetes, as follows from their high abundance. The use of Leymus sabulosus (Bieb.) Tzvel in biological remediation of technozems formed in tailing dumps ensures an increase not only in the abundance of microorganisms in edaphotopes but also in their ecological diversity, compared to technozems planted with Rhus hirta (L.) Sudw, as confirmed by 1.2- and 1.9-fold increase in the Margalef and Berger–Parker diversity indices. Therefore, applying a layer of chernozem on technozems of tailing dumps provides for more efficient remediation of technogenic soils.     

Ecological aspects of humus formation and dynamics in virgin and plowed chernozem soils of the Cisural region

An attempt has been made to make a comprehensive assessment of biological factors in the ecology of humus formation in forest-steppe and steppe chernozem soils of the Cisural region. Certain regional features in the effect of vegetation structure and composition and soil microbial and enzymatic activities on the humus state of zonal chernozem subtypes in virgin and plowed landscapes have been revealed.     

Can the byssus of green-lipped mussel Perna viridis (Linnaeus) from the west coast of Peninsular Malaysia be a biomonitoring organ for Cd,Pb and Zn? Field and laboratory studies

Concentrations of cadmium (Cd), lead (Pb) and zinc (Zn) in total soft tissues (ST) and byssus (BYS) of the green-lipped mussel Perna viridis from 11 different geographical locations off the west coast of Peninsular Malaysia were determined. The metal concentrations distributed between the BYS and ST were compared. The results of this study indicated that higher levels of Cd (1.31 microg/g), Pb (38.49 microg/g) and Zn (206.52 microg/g) were accumulated in the BYS than in the total ST (Cd: 0.29 microg/g; Pb: 8.27 microg/g; Zn: 102.6 microg/g). Semi-static and short period controlled laboratory experiments were also conducted for the accumulation and depuration of Cd, Pb and Zn in the total ST and BYS of P. viridis. The ratios (BYS/ST) for Pb and Cd from the laboratory experiments showed that the total ST accumulated more metals than the BYS. Therefore, these laboratory results disagreed with those found for the field samples. However, the laboratory results for the Zn ratio (BYS/ST) agreed with those of the field samples. It was evident that when compared to the ST, the BYS was a more sensitive biomonitoring organ for Zn while it could be a complementary organ for Cd and Pb in the total ST. Since total ST of P. viridis had been reported to have regulative mechanism for Zn, its BYS can be used as a biomonitoring organ for the identification of coastal areas exposed to Zn pollution.     

Soil as a factor of vegetation regeneration in natural pastures

Data are presented that show the significance of the humus state of chernozem soils, their amphiphilic (hydrophobic-hydrophilic) components, and related physical properties (density, structural condition, and water permeability) for regeneration of natural vegetation and restoration of its species com-position in pasture ecosystems.     

Effect of forest on the composition and properties of organic matter in steppe chernozem soils of neighboring biogeocenoses

Results are presented that have been obtained in studies on the effect of forests growing in the steppe zone on the conditions of formation and qualitative and quantitative composition of organic matter and on the properties of humus in chernozem soils of neighboring biogeocenoses.     

Effects of readily dispersible colloid on adsorption and transport of Zn, Cu, and Pb in soils

Soil colloids (<0.002 mm) were extracted from three types of soils to make the colloid-bound forms of Zn, Cu, and Pb solution. The clay mineral types and composition of the colloids, the adsorption characteristics of the colloids, and the effect of readily dispersible colloid on the transport of metals and the quality of the soils and groundwater were studied. The results showed that the adsorption capacity of Cu, Zn, and Pb was greater for the Aquic Vertisols (Shajiang Black soil) as compared to the Udic Luvisols (Brown soil) and Usdic Luvisols (Cinnamon soil), due to the difference of clay content and clay mineral composition in the different soils. The adsorption capacity of Pb was much higher than that of Zn and Cu for the same soils, which would contribute to the chemical properties of metals and specific adsorption characters of the colloids. The mobility of Zn in soils was greater than that of Cu and Pb, while similar trend was found in the transportation processes for Zn and Cu. The concentration of Zn and Cu in leachates increased as the leaching solution volume increased, but the migration of Pb was negligible, and the concentration of Pb could not been detected in leachates even after 7.5 pore volume leaching solution. The influence of mobility on Zn and Cu transport was different for different type of colloids. The mobility caused by readily dispersible colloids from Aquic Vertisols was greater as compared to that of Udic Luvisols and Usdic Luvisols. Analysis of soils after column leaching indicated that Zn was distributed much deeper than Cu, but Pb was almost not migrated, and mainly accumulated in the soil surface. Therefore, Zn had greater tendency for the groundwater pollution than Cu and Pb, and Pb tends to contaminate the surface soils.     

Biological properties of south Russian soils: Tolerance to oil pollution

Oil pollution of south Russian soils usually leads to reduction in the abundance of ammonifying bacteria, microscopic fungi, and bacteria of the genus Azotobacter, as well as in enzyme (catalase and invertase) and cellulolytic activities. By the criterion of biological tolerance to oil pollution, these soils can be arranged in the following series: ordinary chernozems >= soddy calcareous soils = southern chernozems >= leached chernozems > brown forest soils >= gray sand soils. A regional scheme of ecological safety standards for oil contents in these soil types is proposed.     

Sensitivity of some groups of ordinary chernozem soil microorganisms to γ irradiation

The effect of γ irradiation at doses of 1, 5, 10, and 20 kGy on the microflora of ordinary chernozem soil has been studied. The time course of the restoration of the microorganism numbers over 180 days after γ irradiation has been studied in model experiments. The microorganism radioresistance decreases in the following order: amylolytic bacteria > Azotobacter > spore-forming bacteria > ammonifiers > micromycetes. A dose of 20 kGy has a 96% suppressing effect on all microorganisms. Model experiments on the restoration of the irradiated soil microflora have revealed different rates of increase in the numbers of microorganism groups depending on the dose and incubation time.     

The relationship of dissolved Pb to some dissolved trace metals (Al, Cr, Mn, and Zn) and to dissolved nitrate and phosphate in a freshwater aquatic system in Mauritius

The relationship of some dissolved trace metals (Al, Cr, Mn, Zn, and Pb) with one another and to dissolved phosphate and nitrate in a freshwater aquatic system at Flic en Flac and Grand River North West (GRNW) in Mauritius (1850 km2, 20 degrees S and 57 degrees E, Western Indian ocean) is reported following trace metal determination using inductively coupled plasma mass spectrometry (ICP-MS). Dissolved Al (<200 ng ml(-1)), Cr (<50 ng ml(-1)), Mn (<50 ng ml(-1)), Zn (< 100 ng ml(-1)), and Pb (<50 ng ml(-1)) upstream, downstream GRNW and in the marshes and rivulet at Flic en Flac were found to be below the recommended EEC maximum admissible concentrations and within the ambient drinking water quality standards in Mauritius. Dissolved Pb was significantly positively correlated to both dissolved Cr and Zn suggesting that the cycling for dissolved Pb is linked to the cycling of both dissolved Cr and Zn along GRNW. The common influential cycling of Pb and Zn was further reinforced as both dissolved Pb and Zn were significantly positively correlated to dissolved phosphate, which suggested a biological role in the cycling of Zn and Pb. The role of biological activity or adsorption to biological systems in Pb cycling along GRNW is further suggested as dissolved Pb was significantly correlated to dissolved nitrate. The apparent absence of the dissolved Al, Cr, and Mn with dissolved nitrate and phosphate could be attributed to factors such as the lower sensitivity of the GRNW to metal uptake during biological activity during the time frame considered. The cycling of dissolved Al and Mn was also not linked to the cycling of Cr, Zn, and Pb as no significant correlation was found along GRNW.     

Critical compilation and review of plant/soil concentration ratios for uranium,thorium and lead

The transfer of natural radionuclides of the U decay series through the biosphere is important, especially for assessment of the impacts of mining and milling of U ores. The pathway from soil through plants to humans can contribute significantly to the overall dose received from these radionuclides. Element-specific concentration ratios (CRs) used to model the impact of radionuclides have been reported for U, Th and Pb but have not received the research or critical review accorded other radionuclides associated with the nuclear industry. This paper compiles and analyses many of the available data on CRs for U, Th and Pb and recommends values appropriate for environmental assessments. A brief overview of the statistical properties of CR values and the complex soil/plant processes encompassed by this ratio is also given, as well as an overview of some of the physical, chemical and biological factors likely to influence CR values.Our analysis showed that CR values decreased significantly as the corresponding soil concentrations increased. Although the variability was substantial, with ranges of 1000- to 30 000-fold, CR values did differ significantly among some soil and plant types. The overall geometric means were 0·0045, 0·0036 and 0·052 for U, Th and Pb, respectively.     

Soil-to-plant halogens transfer studies 1. Root uptake of radioiodine by plants

Long-term controlled experiments under natural conditions in the field have been carried out in the Chernobyl Exclusion zone in order to determine the parameters governing radioiodine transfer to plants from four types of soils (podzoluvisol, greyzem and typical and meadow chernozem) homogeneously contaminated in the 20-cm upper layer with an addition of (125)I. An absence of (125)I depletion in arable soil layers due to volatilization was noted up to one year after contamination. During one year, depletion due to the vertical migration of radioiodine from the arable layer of each of the soils did not exceed 4% of the total (125)I content. Radioiodine concentration ratios (CR) were obtained in radish roots, lettuce leaves, bean pods, and wheat grain and straw. The highest CR values were observed in podzoluvisol: 0.01-0.03 for radish roots and lettuce leaves, 0.003-0.004 for bean pods and 0.001 for wheat grains. In the other three soils, these values were one order of magnitude lower. The parameters relating to changes in radioiodine bioavailability were determined, based on the contamination dynamics of plants in field conditions.     

MSWI BA treated with Advanced Dry Recovery: a field scale study on materials’ leaching properties

In many European countries, the environmental properties of waste-derived aggregates are mostly assessed based on laboratory leaching tests such as the standardised percolation tests CEN/TS/14405, ISO/TS 21268-3, or DIN 19528. These tests are conducted under specified conditions, which are similar yet somewhat different from realistic field conditions. Therefore, this study aimed to investigate the leaching properties of ADR (Advanced Dry Recovery) recovered MSWI BA in the field in order to understand more about its environmental impacts in actual civil engineering structures. Two field scale studies (a lysimeter and a larger interim storage field study) were constructed and the leachate quality was investigated. These results were then complemented with the results of previously conducted laboratory leaching tests using a liquid to solid ratio (L kg^?1) comparison. The results demonstrated that the leaching behaviours of many potentially harmful substances, such as chloride, copper and antimony, was similar despite the study scale. In addition, this study illustrated the importance of investigating the leaching properties of waste-derived aggregates on a larger scale, even though the uncertainties in such studies may not be easily controlled.     

Effects of a spill of bunker oil on the marine biological communities in Hong Kong

The effects of a recent bunker oil spill on the marine environment were assessed through investigation of the rocky shore fauna, phytoplankton population and macrobenthic communities over a study period of 150 days. In addition, toxicity experiments were carried out in the laboratory to ascertain the toxic effects of the oil-plus-dispersant on selected test organisms. The impacts of the spill on the marine fauna were minimal with no visible reduction in species and individual numbers. Possible reasons were the small amount of oil spilled, the rapid containment and dispersion in the clean-up operations, and the less toxic effects of the heavy bunker oil. On Hong Kong shores, the limpets can be identified as indicator species to oil pollution. A quick survey of the limpet fauna on the rocky shores immediately after a spill provides an initial assessment of the impacts on the shoreline. However, faunal recovery over a long-term period may be difficult to assess in view of the lack of baseline data on most of the marine biological communities in Hong Kong waters.     

Conversion factors for external gamma dose derived from natural radionuclides in soils

Field in situ gamma radiation exposure rates and laboratory measured radioactivity contents of 1500 Spanish soils were compared. The main objective was to determine if published theoretically derived conversion factors would yield accurate quantitative activity concentration (Bq kg(-1)) for the data carried out in different surveys developed by our laboratory during the last ten years. The in situ external gamma dose rate results were compared to laboratory gamma analysis of soils samples gathered from each site, considering the concentrations of seven radionuclides: 40K, 214Pb, 214Bi, 212Bi, 212Pb, 208Tl and 228Ac. The coefficient of correlation found between these variables indicate a good relationship. A discussion of the factors contributing to the uncertainties as well as measurement procedure are also given in this paper.     

Comprehensive Analysis of Radioactive and Chemical Contamination of Water Bodies in the Area of Radium-Industry Waste Storage

Genotoxicity and cytotoxicity of water samples from the creeks flowing through the area of radium mining waste storage into the Ukhta River and from this river were assessed using biological tests with Allium schoenoprasum L. Chemical analysis of the samples indicated that concentrations of parent heavy natural radionuclides, ²¹⁰Pb, and ²¹⁰Po do not exceed the allowable level. Of ten heavy metals, only Zn and Mn concentrations exceeded MACs. The results of biological tests provided evidence for the significantly increased genotoxicity of water samples. The mitotic index increased with an increase in the concentration of Zn ions and manifested an inverse dependence on the activity of ²³⁸U. The genotoxic effect linearly depended on Zn concentration. Possible mechanisms responsible for the induction of these biological effects are discussed.     

Soil-to-plant halogens transfer studies 2. Root uptake of radiochlorine by plants

Long-term field experiments have been carried out in the Chernobyl exclusion zone in order to determine the parameters governing radiochlorine (36Cl) transfer to plants from four types of soil, namely, podzoluvisol, greyzem, and typical and meadow chernozem. Radiochlorine concentration ratios (CR) in radish roots (15+/-10), lettuce leaves (30+/-15), bean pods (15+/-11) and wheat seed (23+/-11) and straw (210+/-110) for fresh weight of plants were obtained. These values correlate well with stable chlorine values for the same plants. One year after injection, 36Cl reached a quasi-equilibrium with stable chlorine in the agricultural soils and its behavior in the soil-plant system mimicked the behavior of stable chlorine (this behavior was determined by soil moisture transport in the investigated soils). In the absence of intensive vertical migration, more than half of 36Cl activity in arable layer of soil passes into the radish, lettuce and the aboveground parts of wheat during a single vegetation period.     

Cobalt and secondary poisoning in the terrestrial food chain: data review and research gaps to support risk assessment

Cobalt is a naturally occurring element found in rocks, soil, water, plants, and animals and has diverse industrial importance. It is cycled in surface environments through many natural processes (e.g. volcanic eruptions, weathering) and can be introduced through numerous anthropogenic activities (e.g. burning of coal or oil, or the production of cobalt alloys). The environmental behaviour of cobalt in terrestrial environment is relatively poorly studied and in particular where Co is used in industrial processes, the baseline information to support wider and long-term environmental impacts is widely dispersed. To support the adoption of new EU regulations on the risk assessment of chemicals, we review here the various aspects of the environmental chemistry, fate and transport of Co across environmental interfaces and discuss the toxicology and potential for bio magnification and food chain accumulation. The soil-to-plant transfer of Co appears to be viable route to expose lower trophic levels to biologically significant concentrations and Co is potentially accumulated in biomass and top soil. Evidence for further accumulation through soil-invertebrate transfer and to higher trophic levels is suggested by some studies but this is obscured by the relatively high variability of published transfer data. This variation is not due to one particular aspect of the transfer of Co in terrestrial environments. Influences are from the variability of geological sources within soil systems; the sensitivity of Co mobility to environmental factors (e.g. pH) and the variety of life strategies for metal elimination/use within biological species. Toxic effects of Co have been suggested for some soil-plant animal studies however, uncertainty in the extrapolation from laboratory to field is a major limitation.