Physiological and Biochemical Effects of Simulated Acid Rain on Phaseolus Vulgaris Var. HUR-15

Pot experiments were performed to determine the effects of simulated acid rain on Phaseolus vulgaris var. HUR-15. Seeds of Phaseolus vulgaris var. HUR-15 were exposed to simulated acid rain at pH values of 5.1, 4.1, 3.1, 2.1 and 1.1 and a control level of pH 6.8. Plants were harvested at three stages, i.e. pre, peak and post-flowering stages. Seedlings succumbed at pH 1.1. Necrosis developed on the leaves of plants treated with water of pH 2.1, resulting in adverse effect on chlorophyll content. They died after peak-flowering. Root and shoot length and dry weight were reduced with the lowering in pH. Carbohydrate, nitrogen and protein contents decreased with the decrease in pH level, adversely affecting their nutrient value.     

Influence of Landfill Gas on the Microdistribution of Grass Establishment Through Natural Colonization

Many revegetated landfills have poor cover including bare areas where plants do not grow. This study, on the Bisasar Road Landfill site in South Africa, assessed grass species preferences to microhabitat conditions in a mosaic of patches of well-established grassed areas and bare, nonvegetated areas. Factors, including soil CO₂, CH₄, O₂, nutrients, and other general soil conditions, were measured in relation to species distribution and grass biomass in the field. Cynodon dactylon was the dominant grass in the established grass areas but was less abundant in the areas bordering the bare areas where Paspalum paspalodes and Sporobolus africanus were common. A number of soil factors measured were significantly correlated with grass biomass and these included Mg, Ca, Zn, Mn, K, temperature, moisture, and CO₂. However, a laboratory bioassay using the growth of C. dactylon with soils removed from the landfill indicated that there were no differences in the soils from the bare areas and those that supported high plant biomass. Thus, no nutrient deficiency or chemical toxicity was inherent in the soil in the laboratory. The results of the field investigation and bioassay indicated that soil CO₂ as a result of landfill gas infiltration into the root zone was probably the main factor causing bare areas on the landfill where no grass species could colonize and grow and that C. dactylon was more sensitive to elevated soil CO₂ than other grass species such as P. paspalodes and S. africanus.     

The microbial status of natural waters in a protected wilderness area

Waters derived from remote 'wilderness' locations have been assumed to be largely free of bacterial contamination and thus such, near-pristine, protected catchments, unused for agriculture, have been first in the multiple line of protection (pristine catchment-long storage-treatment-disinfection) employed by the water industry. This assumption is challenged by a bacterial survey of the waters derived from the New Cairngorm National Park, Scotland. Over 480 spot samples were taken for 59 sites between March 2001 and October 2002 during nine field campaigns each of three to five days duration. Over 75% of samples tested positive for Escherichia coli (E. coli) and 85% for total coliforms. Concentrations displayed both temporal and spatial patterns. Largest values occurred over the summer months and particularly at weekends at sites frequented by visitors, either for 'wild' camping or day visits, or where water was drawn from the river for drinking. Overall the spatial and temporal variations in bacterial concentrations suggest a relationship with visitor numbers and in particular wild camping. The implications of the results for drinking water quality and visitors health are discussed along with possible management options for the area in terms of improving the disposal of human waste.     

The Effects of Dust on the Federally Threatened Valley Elderberry Longhorn Beetle

We combined a natural experiment with field surveys and GIS to investigate the effects of dust from recreational trails and access roads on the federally threatened Valley elderberry longhorn beetle (“VELB,” Desmocerus californicus dimorphus) and its host plant, elderberry (Sambucus mexicana). Dust is listed in the species recovery plan as a threat to the VELB and unpaved surfaces are common throughout the riparian corridors where the VELB lives, yet the effects of dust on the VELB have been untested. We found that dust deposition varied among sites and was highest within 10 m of trails and roads, but was similar adjacent to dirt and paved surfaces within sites. Elderberry density did not differ with distance from dirt surfaces. Despite similar within-site dust levels, elderberry adjacent to paved surfaces were less stressed than those near dirt ones, possibly because increased runoff from paved surfaces benefited elderberry. Dust deposition across sites was weakly correlated with elderberry stress symptoms (e.g., water stress, dead stems, smaller leaves), indicating that ambient dust (or unmeasured correlates) influenced elderberry. Direct studies of the VELB showed that its distribution was not negatively affected by the proximity to dirt surfaces. Dust from low traffic dirt and paved access roads and trails, therefore, affected VELB presence neither directly nor indirectly through changed elderberry condition. These results suggest that the placement of VELB mitigation, restoration, and conservation areas can proceed independently of access roads if dust and traffic levels do not exceed those in our study site. Furthermore, dust control measures are likely to be unnecessary under such conditions. The potential effects of increased traffic and dust levels are addressed through a literature review.     

Effects of Excess Copper on Growth of Wild Rice (<Emphasis Type="Italic">Zizania palustris</Emphasis>) Seedlings Tested in Reconstituted and Natural Waters

Dissolved copper was toxic to wild rice (Zizania palustris) seedlings when exposed in water from the seed collection site of Swamp Creek, Crandon, Wisconsin, USA, and in laboratory-prepared artificial or reconstituted water. Seeds for the study were harvested, then held through a portion of dormancy, in Swamp Creek. After 60 days they were shipped to a laboratory, chilled, and tested with copper after germination. The end point of the tests was net gain in wet weight of the seedlings; additionally, a pronounced reduction in root development was observed. Using measured concentrations, the lowest no-observable-effect concentration (NOEC) in our study was 37 g/liter in Swamp Creek water and the lowest-observable-effect concentration (LOEC) was 59 g/liter. However, it appeared that there was a point at which concentrations of copper above 400 g/liter did not result in any measurable effect or exhibit a definitive dose–response. Because the results in Swamp Creek water were more relevant to the possibility of local metals additions and the association of reduced seedling growth by copper was more powerful in this water, we derived an equation to express the relationship between copper concentration and toxicity for Swamp Creek water. As an example, we would expect a 3.0% reduction in seedling growth at 5.0 g/liter copper. Seedling roots were particularly affected and the resultant plants may be less well anchored and more susceptible to dislodging than plants not exposed to copper. Further refinement of the methodology may be used to address effects of other contaminants impacting rice beds in North America. This study was conducted while the first author was employed by the U.S. Geological Survey, Mid-continent Ecological Science Center, Fort Collins, Colorado     

Effect of oxygenated liquid additives on the urea based SNCR process

An experimental investigation was performed to study the effect of oxygenated liquid additives, H₂O₂, C₂H₅OH, C₂H₄(OH)₂ and C₃H₅(OH)₃ on NO_x removal from flue gases by the selective non-catalytic reduction (SNCR) process using urea as a reducing agent. Experiments were performed with a 150 kW pilot scale reactor in which a simulated flue gas was generated by the combustion of methane operating with 6% excess oxygen in flue gases. The desired levels of initial NO_x (500 ppm) were achieved by doping the fuel gas with ammonia. Experiments were performed throughout the temperature range of interest, i.e. from 800 to 1200 °C for the investigation of the effects of the process additives on the performance of aqueous urea DeNO_x. With H₂O₂ addition a downward shift of 150 °C in the peak reduction temperature from 1130 to 980 °C was observed during the experimentation, however, the peak reduction efficiency was reduced from 81 to 63% when no additive was used. The gradual addition of C₂H₅OH up to a molar ratio of 2.0 further impairs the peak NO_x reduction efficiency by reducing it to 50% but this is accompanied by a downward shift of 180 °C in the peak reduction temperature. Further exploration using C₂H₄(OH)₂ suggested that a 50% reduction could be attained for all the temperatures higher than 940 °C. The use of C₃H₅(OH)₃ as a secondary additive has a significant effect on the peak reduction efficiency that decreased to 40% the reductions were achievable at a much lower temperature of 800 °C showing a downward shift of 330 °C.     

Effect of aspect on the structure of some natural stands of Abies pindrow in Himalayan moist temperate forest

The effects of aspect, altitude, and slope have been observed on the moist temperate forests of Abies pindrow Spach in the Garhwal Himalaya, India. Four aspects, namely, north-east, north-west, south-east and south-west, have been studied to understand the growth behaviour of A. pindrow at varying altitudes. The total basal cover was found to be highest (5099.6 cm²/100 m²) on the north-east facing slopes, and lowest (3092.7 cm²/100 m²) on the south-east facing slopes. The A. pindrow was found to be associated with Quercus semecarpifolia as a main companion species on all the faces, whereas, Lyonia ovalifolia, was associated only on the west facing slopes.     

Effect of selected and non-selected urban waste compost on the initial growth of corn

Brazil produces approximately 242,000 t of waste per day, 76% of it being dumped outdoors and only 0.9% recycled, including composting, which is an alternative still little known in Brazil. In search of a better destination for residues produced by domestic activities, composting stands as a feasible alternative. Organic compost from waste may be used for various purposes, among which are soil recovery, commercial production, pastures, lawns and reforestry and agriculture. However, the quality of the compost determines the growth and the development of plants. The effect of compost made from urban waste on corn plant (Zea mays L.) growth was investigated. Two types of compost were used: the selected compost (SC), produced from organic waste selectively collected; and the non-selected compost (NSC), taken from a 15-year-old cell from the Canabrava land-fill, located in Salvador, Bahia, Brazil (altitude 51 m, 12°22′–13°08′S, 38°08′–38°47′W). Corn was seeded in polyethylene pots, with soil-compost mixing substrate in the proportion of 0, 15, 30, 45 and 60 t ha⁻¹ equivalent doses. Chemical analyses of the compost and growth properties of the plant like chlorophyll content; height and stem diameter; aerial and radicular dry biomasses, were used to evaluate compost quality. Plants cultivated with SC presented a superior gain, being of 52.5% in stem diameter, 71.1 and 81.2% in root and stem biomasses, respectively. Chlorophyl content alterations were observed in plants from treatments using 30 t compost ha⁻¹ dose onwards. Conventional and multivariate statistical methods were used to evaluate these results. The beneficial action of organic compost in plant growth was confirmed with this research.     

The direct influence of ship traffic on atmospheric PM2.5, PM10 and PAH in Venice

The direct influence of ship traffic on atmospheric levels of coarse and fine particulate matter (PM_2.5, PM₁₀) and fifteen polycyclic aromatic hydrocarbons (PAHs) has been estimated in the urban area of Venice. Data analysis has been performed on results collected at three sites over the summer, when ship traffic is at a maximum. Results indicate that monitoring of the PM daily concentrations is not sufficiently detailed for the evaluation of this contribution, even though it could be useful for specific markers such as PAHs. Therefore a new methodology, based on high temporal resolution measurements coupled with wind direction information and the database of ship passages of the Harbour Authority of Venice has been developed. The sampling sites were monitored with optical detectors (DustTrack^® and Mie pDR-1200) operating at a high temporal resolution (20s and 1s respectively) for PM_2.5 and PM₁₀. PAH in the particulate and gas phases were recovered from quartz fibre filters and polyurethane foam plugs using pressurised solvent extraction, the extracts were then analysed by gas chromatography- high-resolution mass spectrometry. Our results shows that the direct contribution of ships traffic to PAHs in the gas phase is 10% while the contribution to PM_2.5 and to PM₁₀ is from 1% up to 8%.     

The Effects of Site Conditions and Mitigation Practices on Success of Establishing the Valley Elderberry Longhorn Beetle and Its Host Plant,Blue Elderberry

This study performed the first systematic evaluation of the success of habitat mitigation at establishing the threatened Valley elderberry longhorn beetle (Desmocerus californicus dimorphus) and its host plant, blue elderberry (Sambucus mexicana). Habitat mitigation performed through enforcement of the U.S. Endangered Species Act represents a tightly controlled form of habitat restoration, facilitating the evaluation of restoration practice. Restoration plantings of blue elderberry have been substantial in our study area, the Central Valley of California. Surveys of 30 mitigation sites and 16 nearby natural sites showed that mitigation sites were a fraction of the size of natural habitat areas (mean = 24%) and contained smaller shrubs. The beetle colonized 53% of mitigation sites and its populations were denser in sites with moderate levels of dead stems on elderberry shrubs, and moderate damage to elderberry stems and bark. This likely indicates that the beetle responds to stressed shrubs, which are likely to contain elevated levels of nitrogen. Beetle density also increased with the size and age of mitigation sites. This indicates a need to make restoration sites as large as possible and to monitor these sites for longer than current guidelines suggest, thereby allowing more time for convergence of natural and mitigation sites. Few factors examined here directly influenced the growth of elderberry shrubs, but elderberry grew more rapidly in sites closer to riparian areas, indicating that such sites should be favored for mitigation sites.     

Optimizing vermicomposting of animal wastes: effects of rate of manure application on carbon loss and microbial stabilization

Vermicomposting is the process whereby organic residues are broken down by earthworms and microorganisms. Addition of manure has been shown to be of critical importance and determines most of the changes that take place during vermicomposting. Here, we study how the rate of manure applied affects microbial biomass and activity and carbon losses. For this, we designed continuous feeding reactors in which new layers of manure were added sequentially to form an age gradient inside the reactors. We compared two application rates of pig slurry (1.5 and 3kg) and set up six reactors for each one; half of the 12 reactors initially contained a population of 500 earthworms (Eisenia fetida). We found that earthworms increased microbial biomass and were more active in reactors fed with 3kg of slurry. However, the differential rates of respiration were not reflected in C losses. The results thus showed that loss of C was not affected by the rate of pig slurry applied. We conclude that despite the strong effect that the rate of manure has on microbe-earthworm relationships, it did not affect carbon losses. We therefore recommend the use of low application rates of manure when the objective is the microbial stabilization of the residue.     

Effect of Cattle Grazing and Seedling Size on the Establishment ofAraucaria cunninghamiiin a Silvo-pastoral System in Northeast Australia

A study was undertaken to assess the effect of tree age at which grazing commenced and seedling size on the establishment ofAraucaria cunninghamii(hoop pine) trees in a silvo-pastoral system on the Atherton Tablelands of northeast Australia. Grazing with dairy cattle commenced at three ages after tree planting: (1) 2 weeks; (2) 6 months and (3) 18 months. Seedlings of two size classes, small (15 cm tall and 18 months old) and large (45 cm tall and 27 months old), were used. Trees were measured for survival and height, and assessed for physical damage and development of multi-stems at ages 6, 12 and 24 months. Results showed that tree survival and height did not differ significantly with age at which grazing commenced and that grazing did not cause unacceptable mortality, damage or reduction in height growth ofA. cunninghamii. The lack of cattle impact was mainly becauseA. cunninghamiihas a special morphological feature, prickly needles, which discourages cattle from physical contact. Although tree height ofA. cunninghamiivaried significantly between the two seedling sizes, the difference became smaller as the trees grew. It is suggested that for the establishment ofA. cunninghamiiin silvo-pastoral systems, grazing can be allowed almost immediately after tree planting, providing an appropriate stocking rate is used. There are no apparent advantages in using larger, compared with smaller, seedlings.     

Effect of bioremediation on the growth of Okro (<Emphasis Type="Italic">Abelmoshus esculetus</Emphasis>) in the Niger Delta soils

This paper communicates the effect of bioremediation on the performance of Okro plant (Abelmoshus esculentus) in a typical Niger Delta soil that has received 5% crude oil pollution level. Biodegrading bacteria such as Pseudomonas fluorescen, Acinetobacteria iwofii, Bacillus subtilus, Arthrobacter globiformis that was isolated from previously polluted soils was introduced into the samples. The treatment combinations are as follows (A) = control without crude oil; B = soil + crude oil, (C) = soil + crude oil + microbes, (D) = soil + crude oil + microbes, (E) = soil + crude oil + microbes + fertilizer (F) soil + microbes and (G) = soil + fertilizer. The treatment (E) gave the highest number of leaves, % crop emergence, plant biomass, microbial population and degradation of petroleum hydrocarbon compared to any of the treatments that had received crude oil. This suggested that fertilizer application does not only stimulate microbial growth but it provides the plant with more available nutrients required for plant growth.     

Effect of spent engine oil on the growth parameters and chlorophyll content of <Emphasis Type="Italic">Corchorus olitorius</Emphasis> Linn

The effect of spent engine oil on the height, leaf number, leaf area, stem girth, chlorophyll, and moisture contents of Corchorus olitorius grown on 0, 0.2%, 0.4%, 0.6%, 0.8%, 1.0%, 2.0%, and 3.0% (v/w oil/soil) oil-contaminated soil was investigated. The engine oil at all concentrations delayed the germination of C. olitorius by 2 days (compared to control) and there was a general significant reduction in all the growth parameters in plants grown on contaminated soil compared to control plants. The highest leaf area of 26.8 cm² was found in the control plant and least was found in the 0.6% soil (0.11 cm²) after 3 weeks while no values were recorded on the 0.8–3.0% engine-oil-contaminated soil after 5 weeks of experiment. The highest chlorophyll content was also found in the control plant (11.5 mg/l). This showed that spent engine oil has an adverse effect on the growth of C. olitorius plant.     

Evaluation of fire as a management tool for controllingSchinus terebinthifolius as secondary successional growth on abandoned agricultural land

Schinus terebinthifolius, native to South America, has become an aggressive woody weed in southern Florida, displacing native vegetation as well as rapidly invading disturbed sites. Studies to evaluate the effectiveness of fire as a management option for controllingSchinus on abandoned farmland in Everglades National Park, known as the “Hole-in-the-Donut,” began in 1979. Study plots were established to monitor any change(s) in herbaceous cover and in numbers and size ofSchinus stems. Except in the control plot (which was not burned), each site was burned as often as fuel conditions permitted (usually once every one or two years), through 1985. Results indicated that both the number and density ofSchinus stems increased over the course of the study. While plots that burned showed a reduction in the rate ofSchinus invasion, invasion still progressed rapidly with or without the occurrence of fire. The increase inSchinus stem density from 1980 to 1985 was highly significant in all transects except one. Herbaceous cover showed no clear trends relative to burning.     

Effect of ceramic industrial particulate emission control on key components of ambient PM10

The relationship between specific particulate emission control and ambient levels of some PM₁₀ components (Zn, As, Pb, Cs, Tl) was evaluated. To this end, the industrial area of Castellón (Eastern Spain) was selected, where around 40% of the EU glazed ceramic tiles and a high proportion of EU ceramic frits are produced. The PM₁₀ emissions from the ceramic processes were calculated over the period 2000–2006, taking into account the degree of implementation of corrective measures throughout the study period. Abatement systems were implemented in the majority of the fusion kilns for frit manufacture in the area as a result of the application of the Directive 1996/61/EC, leading to a marked decrease in PM₁₀ emissions. By contrast, emissions from tile manufacture remained relatively constant because of the few changes in the implementation of corrective measures. On the other hand, ambient PM₁₀ levels and composition measurements were carried out from 2002 to 2006. A high correlation between PM₁₀ emissions from frit manufacture and ambient levels of Zn, As, Pb and Cs (R² from 0.61 to 0.98) was observed. On the basis of these results, the potential impact of the implementation of corrective measures to reduce emissions from tile manufacture was quantified, resulting in a possible decrease of 3–5 μg/m³ and 2 μg/m³ in ambient mineral PM₁₀ (on an annual basis) in urban and suburban areas, respectively. This relatively simple methodology allows us to estimate the direct effect of a reduction in primary particulate emissions on ambient levels of key particulate components, and to make a preliminary quantification of the possibilities of air quality improvement by means of further emission reduction. Therefore, it is a useful tool for developing future air quality plans in the study area and in other industrialised areas.     

Effect of stocking density and food quality on the growth and fecundity of an epigeic earthworm (<Emphasis Type="Italic">Eisenia fetida</Emphasis>) during vermicomposting

Physico-chemical characteristics of the feed and optimum worm density are important parameters for the efficient working of a vermicomposting system. Overcrowding of worms can affect the efficiency of a vermicomposting system even if all other parameters have been optimized. This article reports the effect of stocking density and feed quality on the growth and fecundity of Eisenia fetida under laboratory conditions. The feed mixtures contained cow dung and textile mill wastewater sludge in different ratios. Three feed mixtures and five stocking rates (1, 2, 4, 8, and 12) were tested for 12 weeks. The results showed that E. fetida growth rate was faster at higher stocking densities; however, biomass gain per worm was faster at lower stocking densities. Sexual maturity was attained earlier at higher stocking densities. Growth rate was highest in 100% cow dung at all the stocking densities when compared to textile mill wastewater sludge containing feed mixtures. A worm population of 27–53 worms per kg of feed was found to be the most favorable stocking density.